BRENDA - Enzyme Database show
show all sequences of 1.1.1.198

Biosynthesis of monoterpenes. Partial purification and characterization of a bicyclic monoterpenol dehydrogenase from sage (Salvia officinalis)

Croteau, R.; Hooper, C.L.; Felton, M.; Arch. Biochem. Biophys. 188, 182-193 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(+)-Camphor
27% inhibition at 0.5 mM
Salvia officinalis
(-)-Thujol
competitive inhibition of (+)-borneol oxidation
Salvia officinalis
8-hydroxyquinoline
57% inhibition at 1 mM
Salvia officinalis
Borate
inhibition of borneol oxidation
Salvia officinalis
Cu2+
85% inhibition at 2 mM
Salvia officinalis
ethylenediaminetetraacetate
slight inhibition at 1 mM
Salvia officinalis
Hg2+
nearly complete inhibition at 2 mM
Salvia officinalis
iodoacetamide
41% inhibition at 1 mM
Salvia officinalis
N-ethylmaleimide
53% inhibition at 1 mM
Salvia officinalis
NaCN
slight inhibition at 1 mM
Salvia officinalis
NADH
46% inhibition at 1 mM
Salvia officinalis
NaN3
slight inhibition at 1 mM
Salvia officinalis
o-phenanthroline
slight inhibition at 1 mM
Salvia officinalis
p-hydroxymercuribenzoate
50% inhibition at 0.05 mM
Salvia officinalis
Pb2+
26% inhibition at 2 mM
Salvia officinalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
(+)-borneol
-
Salvia officinalis
0.041
-
(-)-Thujol
-
Salvia officinalis
0.07
-
NAD+
-
Salvia officinalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
gel filtration
Salvia officinalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-borneol + NAD+
Salvia officinalis
B-site stereospecific, partially reversible
(+)-camphor + NADH
-
Salvia officinalis
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salvia officinalis
-
sage
-
Purification (Commentary)
Commentary
Organism
near homogeneity, chromatography techniques, affinity chromatography
Salvia officinalis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Salvia officinalis
-
Storage Stability
Storage Stability
Organism
0°C, 0.1 M sodium phosphate, pH 8.0, 15% glycerol, 20 mM 2-mercaptoethanol, 3 days, 25% loss of activity
Salvia officinalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-borneol + NAD+
B-site stereospecific, partially reversible
286163
Salvia officinalis
(+)-camphor + NADH
-
286163
Salvia officinalis
r
(+)-dihydrocarveol + NAD+
poor substrate
286163
Salvia officinalis
(-)-dihydrocarvone + NADH
-
286163
Salvia officinalis
?
(+)-endo-fenchol + NAD+
26% of the activity compared to (+)-borneol
286163
Salvia officinalis
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
286163
Salvia officinalis
?
(+)-isoborneol + NAD+
nearly the same activity compared to (+)-borneol
286163
Salvia officinalis
(+)-isocamphor + NADH
-
286163
Salvia officinalis
?
(-)-borneol + NAD+
36% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-camphor + NADH
-
286163
Salvia officinalis
?
(-)-endo-fenchol + NAD+
poor substrate
286163
Salvia officinalis
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
286163
Salvia officinalis
?
(-)-isoborneol + NAD+
30% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-isocamphor + NADH
-
286163
Salvia officinalis
?
(-)-neothujol + NAD+
65% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-neothujone + NADH
-
286163
Salvia officinalis
?
(-)-thujol + NAD+
2fold higher activity than for (+)-borneol
286163
Salvia officinalis
(-)-thujone + NADH
-
286163
Salvia officinalis
?
(1R)-menthol + NAD+
poor substrate
286163
Salvia officinalis
(1R)-trans-p-menthan-3-one + NADH
-
286163
Salvia officinalis
?
geraniol + NAD+
poor substrate
286163
Salvia officinalis
geranial + NADH
-
286163
Salvia officinalis
?
nerol + NAD+
poor substrate
286163
Salvia officinalis
neryl aldehyde + NADH
-
286163
Salvia officinalis
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Salvia officinalis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
most stable around
Salvia officinalis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Salvia officinalis
NADP+
25% as efficient as NAD+
Salvia officinalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Salvia officinalis
NADP+
25% as efficient as NAD+
Salvia officinalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(+)-Camphor
27% inhibition at 0.5 mM
Salvia officinalis
(-)-Thujol
competitive inhibition of (+)-borneol oxidation
Salvia officinalis
8-hydroxyquinoline
57% inhibition at 1 mM
Salvia officinalis
Borate
inhibition of borneol oxidation
Salvia officinalis
Cu2+
85% inhibition at 2 mM
Salvia officinalis
ethylenediaminetetraacetate
slight inhibition at 1 mM
Salvia officinalis
Hg2+
nearly complete inhibition at 2 mM
Salvia officinalis
iodoacetamide
41% inhibition at 1 mM
Salvia officinalis
N-ethylmaleimide
53% inhibition at 1 mM
Salvia officinalis
NaCN
slight inhibition at 1 mM
Salvia officinalis
NADH
46% inhibition at 1 mM
Salvia officinalis
NaN3
slight inhibition at 1 mM
Salvia officinalis
o-phenanthroline
slight inhibition at 1 mM
Salvia officinalis
p-hydroxymercuribenzoate
50% inhibition at 0.05 mM
Salvia officinalis
Pb2+
26% inhibition at 2 mM
Salvia officinalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
(+)-borneol
-
Salvia officinalis
0.041
-
(-)-Thujol
-
Salvia officinalis
0.07
-
NAD+
-
Salvia officinalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
gel filtration
Salvia officinalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-borneol + NAD+
Salvia officinalis
B-site stereospecific, partially reversible
(+)-camphor + NADH
-
Salvia officinalis
r
Purification (Commentary) (protein specific)
Commentary
Organism
near homogeneity, chromatography techniques, affinity chromatography
Salvia officinalis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Salvia officinalis
-
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 0.1 M sodium phosphate, pH 8.0, 15% glycerol, 20 mM 2-mercaptoethanol, 3 days, 25% loss of activity
Salvia officinalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-borneol + NAD+
B-site stereospecific, partially reversible
286163
Salvia officinalis
(+)-camphor + NADH
-
286163
Salvia officinalis
r
(+)-dihydrocarveol + NAD+
poor substrate
286163
Salvia officinalis
(-)-dihydrocarvone + NADH
-
286163
Salvia officinalis
?
(+)-endo-fenchol + NAD+
26% of the activity compared to (+)-borneol
286163
Salvia officinalis
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
286163
Salvia officinalis
?
(+)-isoborneol + NAD+
nearly the same activity compared to (+)-borneol
286163
Salvia officinalis
(+)-isocamphor + NADH
-
286163
Salvia officinalis
?
(-)-borneol + NAD+
36% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-camphor + NADH
-
286163
Salvia officinalis
?
(-)-endo-fenchol + NAD+
poor substrate
286163
Salvia officinalis
1,3,3-trimethyl-bicyclo[2.2.1]heptan-2-one + NADH
-
286163
Salvia officinalis
?
(-)-isoborneol + NAD+
30% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-isocamphor + NADH
-
286163
Salvia officinalis
?
(-)-neothujol + NAD+
65% of the activity compared to (+)-borneol
286163
Salvia officinalis
(-)-neothujone + NADH
-
286163
Salvia officinalis
?
(-)-thujol + NAD+
2fold higher activity than for (+)-borneol
286163
Salvia officinalis
(-)-thujone + NADH
-
286163
Salvia officinalis
?
(1R)-menthol + NAD+
poor substrate
286163
Salvia officinalis
(1R)-trans-p-menthan-3-one + NADH
-
286163
Salvia officinalis
?
geraniol + NAD+
poor substrate
286163
Salvia officinalis
geranial + NADH
-
286163
Salvia officinalis
?
nerol + NAD+
poor substrate
286163
Salvia officinalis
neryl aldehyde + NADH
-
286163
Salvia officinalis
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Salvia officinalis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
most stable around
Salvia officinalis
Other publictions for EC 1.1.1.198
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724125
Sarker
Molecular cloning and function ...
Lavandula x intermedia
Arch. Biochem. Biophys.
528
163-170
2012
-
-
1
-
-
-
-
1
-
-
1
-
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1
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2
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2
1
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-
1
-
-
-
2
-
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-
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-
1
2
-
-
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-
-
-
1
-
-
1
-
-
-
-
-
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2
-
-
2
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
726116
Polichuk
A glandular trichome-specific ...
Artemisia annua
Phytochemistry
71
1264-1269
2010
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
6
-
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
6
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286162
Dehal
Metabolism of monoterpenes: Sp ...
Salvia officinalis
Arch. Biochem. Biophys.
258
287-291
1987
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286163
Croteau
Biosynthesis of monoterpenes. ...
Salvia officinalis
Arch. Biochem. Biophys.
188
182-193
1978
-
-
-
-
-
-
15
3
-
-
1
1
-
1
-
-
1
-
-
1
-
1
12
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
15
-
3
-
-
1
1
-
-
-
1
-
1
-
1
12
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-