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Literature summary for 1.1.1.195 extracted from

  • Choi, B.; Chung, J.; Bae, H.; Bae, I.; Park, S.; Bae, H.
    Functional characterization of cinnamyl alcohol dehydrogenase during developmental stages and under various stress conditions in kenaf (Hibiscus cannabinus L.) (2016), BioResources, 11, 105-125.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene cad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree Hibiscus cannabinus
gene cad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Hibiscus cannabinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01425
-
cinnamaldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
0.0282
-
coniferyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
0.0471
-
sinapyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the enzyme contains no N-terminal targeting sequence Hibiscus cannabinus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Hibiscus cannabinus
Zn2+ required, Zn1 binding sequence is GHE(X)2G(X)5G(X)2V located at amino acid residues 68-82, and Zn2 binding sequence GD(X)9,10C(X)2C(X)2(X)7C located at amino acid residues 88-114, structural zinc coordinated residue are Cys100, Cys103, Cys106, and Cys114 Hibiscus cannabinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38660
-
-
Hibiscus cannabinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cinnamaldehyde + NADPH + H+ Hibiscus cannabinus
-
cinnamyl alcohol + NADP+
-
?
coniferyl aldehyde + NADPH + H+ Hibiscus cannabinus
-
coniferyl alcohol + NADP+
-
r
sinapyl aldehyde + NADPH + H+ Hibiscus cannabinus
-
sinapyl alcohol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Hibiscus cannabinus A0A0U1ZF25 CAD2
-
Hibiscus cannabinus D9ZKR7 CAD1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Hibiscus cannabinus

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Hibiscus cannabinus
-
flower highest CAD1 expression level in mature flowers Hibiscus cannabinus
-
leaf
-
Hibiscus cannabinus
-
additional information CAD expression pattern during different developmental stages, overview. HcCAD2 expression level is always higher than the expression level of HcCAD1. High expression levels in early stages of stem development and in mature stage of flowers development Hibiscus cannabinus
-
petiole
-
Hibiscus cannabinus
-
root
-
Hibiscus cannabinus
-
seedling
-
Hibiscus cannabinus
-
stem
-
Hibiscus cannabinus
-
stem highest CAD2 expression level in stems Hibiscus cannabinus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cinnamaldehyde + NADPH + H+
-
Hibiscus cannabinus cinnamyl alcohol + NADP+
-
?
coniferyl aldehyde + NADPH + H+
-
Hibiscus cannabinus coniferyl alcohol + NADP+
-
r
additional information coniferylaldehyde and sinapylaldehyde are the preferred substrates Hibiscus cannabinus ?
-
?
sinapyl aldehyde + NADPH + H+
-
Hibiscus cannabinus sinapyl alcohol + NADP+
-
r

Subunits

Subunits Comment Organism
? x * 38660, sequence calculation Hibiscus cannabinus
More enzyme structure analysis, overview Hibiscus cannabinus

Synonyms

Synonyms Comment Organism
cinnamyl alcohol dehydrogenase
-
Hibiscus cannabinus
HcCAD1
-
Hibiscus cannabinus
HcCAD2
-
Hibiscus cannabinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Hibiscus cannabinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 60 activity range, profile overview Hibiscus cannabinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1437
-
cinnamaldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
7564
-
coniferyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
9368
-
sinapyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
recombinant enzyme, aldehyde reduction Hibiscus cannabinus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 activity range, the enzyme is completely inactive at pH 5.0 and pH 9.0, profile overview Hibiscus cannabinus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Hibiscus cannabinus
NADPH
-
Hibiscus cannabinus
NADPH NADPH binding sequence GXG(X)2G Hibiscus cannabinus

pI Value

Organism Comment pI Value Maximum pI Value
Hibiscus cannabinus sequence calculation
-
5.34

Expression

Organism Comment Expression
Hibiscus cannabinus methyljasmonate, and drought stress suppress CAD1 expression down
Hibiscus cannabinus wounding, methyljasmonate, and cold stress suppress CAD1 expression down
Hibiscus cannabinus H2O2 and drought stress, and slightly also abscisic acid induce CAD1 expression up
Hibiscus cannabinus wounding, cold, H2O2, salt, and drought stresses, salicylic acid, and abscisic acid induce CAD1 expression up

General Information

General Information Comment Organism
metabolism enzyme CAD is involved in the lignin biosynthesis. CAD converts cinnamylaldehyde to cinnamyl alcohol in the final step of the monolignol biosynthesis pathway and is a key enzyme in the pathway. The monolignol biosynthesis is the first major step in lignin biosynthesis, and crosslinkage of monolignols by perocidases and laccases is the second major step Hibiscus cannabinus
additional information lignin and sugar content and composition during stem development, overview Hibiscus cannabinus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100800
-
cinnamaldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
198900
-
sinapyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus
268200
-
coniferyl aldehyde pH 6.0, 30°C, recombinant enzyme Hibiscus cannabinus