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Literature summary for 1.1.1.195 extracted from
- Functional analysis of a cinnamyl alcohol dehydrogenase involved in lignin biosynthesis in wheat (2010), J. Exp. Bot., 61, 2735-2744.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Triticum aestivum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0219 | - |
coniferyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum |  |
| 0.0244 | - |
5-hydroxyconiferyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.0272 | - |
p-coumaryl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.0406 | - |
sinapyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.0891 | - |
caffeoyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38624 | - |
x * 38624, calculated. x * 42500, SDS-PAGE of His-tagged protein | Triticum aestivum |
| 42500 | - |
x * 38624, calculated. x * 42500, SDS-PAGE of His-tagged protein | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | D7PGW0 | isoform TaCAD1 belonging to the bona fide CAD group involved in lignin synthesis | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ | random mechanism | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | low expression | Triticum aestivum | - |
| additional information | no expression in root detectable | Triticum aestivum | - |
| stem | high expression | Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyconiferyl aldehyde + NADPH + H+ | - |
Triticum aestivum | 5-hydroxyconiferyl alcohol + NADP+ | - |
r | |
| caffeoyl aldehyde + NADPH + H+ | - |
Triticum aestivum | caffeoyl alcohol + NADP+ | - |
r | |
| coniferyl aldehyde + NADPH + H+ | best substrate | Triticum aestivum | coniferyl alcohol + NADP+ | - |
r | |
| p-coumaryl aldehyde + NADPH + H+ | - |
Triticum aestivum | p-coumaryl alcohol + NADP+ | - |
r | |
| sinapyl aldehyde + NADPH + H+ | - |
Triticum aestivum | sinapyl alcohol + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38624, calculated. x * 42500, SDS-PAGE of His-tagged protein | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CAD1 | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Triticum aestivum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
about 85% loss of initial activity | Triticum aestivum |
| 60 | - |
complete loss of activity | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
complete loss of activity | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
more than 70% of the maximum activity | Triticum aestivum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
almost complete loss of activity | Triticum aestivum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Triticum aestivum | calculated | - |
5.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Triticum aestivum | mRNA is highly expressed in stems at the elongation and heading stages. At the milky stage of wheat, CAD1 mRNA abundance, protein level and enzyme activity in stem tissues are higher in lodging-resistant cultivar H4546 than in lodging-sensitive cultivar C6001 | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CAD1 is the predominant CAD in wheat stem for lignin biosynthesis and is critical for lodging resistance | Triticum aestivum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.082 | - |
caffeoyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.169 | - |
5-hydroxyconiferyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.25 | - |
p-coumaryl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.286 | - |
sinapyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
| 0.896 | - |
coniferyl aldehyde | pH not specified in the publication, temperature not specified in the publication | Triticum aestivum | |
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Release 2023.1 (January 2023)
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