Cloned (Comment) | Organism |
---|---|
overexpression in strain BL9 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml of both protein and reservoir solution, the latter containing 1. 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, or 2.30% w/v PEG 4000, 0.1 M Tris-HCl, pH 8.5, room temperature, about 1 week, trigonal or monoclinic crystals, X-ray diffraction structure determination and analysis at 3.1 A resolution | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 2 zinc atoms per subunit | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
ScAdh6p | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain BL9 by DEAE ion exchange, Red-Sepharose, and gel filtration chromatography | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cinnamyl alcohol + NADP+ = cinnamaldehyde + NADPH + H+ | active site structure | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
More | analysis of the apo and holo enzyme structures | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
CAD | - |
Saccharomyces cerevisiae |
cinnamyl alcohol dehydrogenase | - |
Saccharomyces cerevisiae |
ScAdh6p | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent for, binding and specificity mechanism, interaction through Ser210, Arg211, and Lys215 and the terminal phosphate group | Saccharomyces cerevisiae |