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show all sequences of 1.1.1.194

Molecular cloning and expression of the Streptomyces coniferyl alcohol dehydrogenase gene in Escherichia coli

Nishimura, M.; Protein Expr. Purif. 89, 109-115 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on
Streptomyces sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Streptomyces sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
M5AJW4
-
-
Streptomyces sp. NL15-2K
M5AJW4
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography
Streptomyces sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
cell-free extract, at pH 8.5 and 40C
Streptomyces sp.
10.7
-
after 40fold purification, at pH 8.5 and 40C
Streptomyces sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferyl alcohol + NADP+
-
739587
Streptomyces sp.
coniferyl aldehyde + NADPH + H+
-
-
-
?
coniferyl alcohol + NADP+
-
739587
Streptomyces sp. NL15-2K
coniferyl aldehyde + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 37845, calculated from amino acid sequence; 4 * 39000, SDS-PAGE
Streptomyces sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Streptomyces sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25.8
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptomyces sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptomyces sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptomyces sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on
Streptomyces sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Streptomyces sp.
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography
Streptomyces sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.27
-
cell-free extract, at pH 8.5 and 40C
Streptomyces sp.
10.7
-
after 40fold purification, at pH 8.5 and 40C
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferyl alcohol + NADP+
-
739587
Streptomyces sp.
coniferyl aldehyde + NADPH + H+
-
-
-
?
coniferyl alcohol + NADP+
-
739587
Streptomyces sp. NL15-2K
coniferyl aldehyde + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 37845, calculated from amino acid sequence; 4 * 39000, SDS-PAGE
Streptomyces sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Streptomyces sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25.8
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptomyces sp.
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
93.3
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
93.3
-
coniferyl alcohol
recombinant enzyme, at pH 8.5 and 40C
Streptomyces sp.
Other publictions for EC 1.1.1.194
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739587
Nishimura
Molecular cloning and expressi ...
Streptomyces sp., Streptomyces sp. NL15-2K
Protein Expr. Purif.
89
109-115
2013
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-
1
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1
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1
1
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6
-
-
1
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2
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2
1
1
-
-
1
1
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1
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-
1
1
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-
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-
-
-
1
-
1
1
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1
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2
-
2
1
1
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-
1
1
-
-
-
-
-
-
-
1
1
667289
Plaggenborg
Potential of Rhodococcus strai ...
Rhodococcus sp., Rhodococcus sp. HR199
Appl. Microbiol. Biotechnol.
72
745-755
2006
-
3
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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3
1
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-
-
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-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
389484
Sauter
-
Levels of beta-glucan and lign ...
Oryza sativa
Plant Cell Physiol.
33
1089-1097
1992
-
-
-
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-
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1
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1
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1
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1
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1
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1
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2
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2
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1
-
1
-
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1
-
1
-
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-
1
-
-
-
-
-
-
-
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-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
-
-
-
-
-
-
-
-
-
-
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1
-
4
-
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-
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1
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1
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1
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1
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1
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389603
Mansell
-
Multiple forms and specificity ...
Acer rubrum, Medicago sativa, Metasequoia glyptostroboides, Pinus palustris, Prunus padus, Salix purpurea, Schinus terebinthifolia, Zea mays
Phytochemistry
15
1849-1853
1976
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-
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8
-
8
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-
-
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8
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8
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8
-
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8
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8
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-
347801
Wyrambik
Purification and properties of ...
Glycine max
Eur. J. Biochem.
59
9-15
1975
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
-
1
1
1
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
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3
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
1
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389481
Ranjeva
Phenolic metabolism in petunia ...
Petunia x hybrida
Biochim. Biophys. Acta
399
23-30
1975
-
-
-
-
-
-
-
-
-
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1
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2
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1
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1
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1
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1
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1
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1
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1
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389482
Gross
-
Three novel enzymes involved i ...
Salix alba
FEBS Lett.
31
283-286
1973
-
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-
-
-
-
-
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-
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1
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1
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1
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2
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2
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1
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1
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