BRENDA - Enzyme Database show
show all sequences of 1.1.1.193

Identification and characterization of two Streptomyces davawensis riboflavin biosynthesis gene clusters

Grill, S.; Yamaguchi, H.; Wagner, H.; Zwahlen, L.; Kusch, U.; Mack, M.; Arch. Microbiol. 188, 377-387 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
Streptomyces davawensis
Engineering
Amino acid exchange
Commentary
Organism
additional information
a ribG mutant can be rescued by expression of ribB, overview
Streptomyces davawensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davawensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davawensis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davawensis 768
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
additional information
Streptomyces davawensis
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
-
additional information
Streptomyces davawensis 768
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces davawensis
Q2VCF5
bifunctional enzyme; strain 768, gene ribG
-
Streptomyces davawensis 768
Q2VCF5
bifunctional enzyme; strain 768, gene ribG
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davawensis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davawensis 768
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davawensis
?
-
-
-
-
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davawensis 768
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davawensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
Streptomyces davawensis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a ribG mutant can be rescued by expression of ribB, overview
Streptomyces davawensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davawensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davawensis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Streptomyces davawensis 768
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
additional information
Streptomyces davawensis
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
-
additional information
Streptomyces davawensis 768
the enzyme is involved in riboflavin biosynthesis, overview
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davawensis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
684754
Streptomyces davawensis 768
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davawensis
?
-
-
-
-
additional information
the enzyme is involved in riboflavin biosynthesis, overview
684754
Streptomyces davawensis 768
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 22000, recombinant enzyme, SDS-PAGE
Streptomyces davawensis
Other publictions for EC 1.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723878
Dawson
Structure of diaminohydroxypho ...
Acinetobacter baumannii
Acta Crystallogr. Sect. F
69
611-617
2013
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1
1
-
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-
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3
-
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1
-
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-
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-
1
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
-
-
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3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684754
Grill
Identification and characteriz ...
Streptomyces davawensis, Streptomyces davawensis 768
Arch. Microbiol.
188
377-387
2007
-
-
1
-
1
-
-
-
-
-
1
4
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246735
Richter
Biosynthesis of riboflavin: ch ...
Bacillus subtilis, Escherichia coli
J. Bacteriol.
179
2022-2028
1997
-
-
2
-
-
-
-
-
-
2
-
2
-
2
-
-
2
-
-
-
-
-
2
2
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
2
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
246736
Burrows
Presence in Escherichia coli o ...
Escherichia coli
J. Bacteriol.
136
657-667
1978
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-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-