BRENDA - Enzyme Database show
show all sequences of 1.1.1.193

Presence in Escherichia coli of a deaminase and a reductase involved in biosynthesis of riboflavin

Burrows, R.B.; Brown, G.M.; J. Bacteriol. 136, 657-667 (1978)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
Escherichia coli
intermediate in the biosynthesis of riboflavin
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
strain B
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Storage Stability
Storage Stability
Organism
-6C, 50 mM Tris-HCl, pH 8.0, 1 month
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
intermediate in the biosynthesis of riboflavin
246736
Escherichia coli
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
-
246736
Escherichia coli
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
30% as effective as NADPH
Escherichia coli
NADPH
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
30% as effective as NADPH
Escherichia coli
NADPH
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
Escherichia coli
intermediate in the biosynthesis of riboflavin
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Escherichia coli
-
Storage Stability (protein specific)
Storage Stability
Organism
-6C, 50 mM Tris-HCl, pH 8.0, 1 month
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-2,6-dioxy-4-(5'-phosphoribosylamino)pyrimidine + NADPH
intermediate in the biosynthesis of riboflavin
246736
Escherichia coli
5-amino-2,6-dioxy-4-(5'-phosphoribitylamino)pyrimidine + NADP+
-
246736
Escherichia coli
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
Other publictions for EC 1.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723878
Dawson
Structure of diaminohydroxypho ...
Acinetobacter baumannii
Acta Crystallogr. Sect. F
69
611-617
2013
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684754
Grill
Identification and characteriz ...
Streptomyces davawensis, Streptomyces davawensis 768
Arch. Microbiol.
188
377-387
2007
-
-
1
-
1
-
-
-
-
-
1
4
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246735
Richter
Biosynthesis of riboflavin: ch ...
Bacillus subtilis, Escherichia coli
J. Bacteriol.
179
2022-2028
1997
-
-
2
-
-
-
-
-
-
2
-
2
-
2
-
-
2
-
-
-
-
-
2
2
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
2
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
246736
Burrows
Presence in Escherichia coli o ...
Escherichia coli
J. Bacteriol.
136
657-667
1978
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-