BRENDA - Enzyme Database show
show all sequences of 1.1.1.193

Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis

Richter, G.; Fischer, M.; Krieger, C.; Eberhardt, S.; Luttgen, H.; Gerstenschläger, I.; Bacher, A.; J. Bacteriol. 179, 2022-2028 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ribD, i.e. ribG, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Escherichia coli
gene ribG, i.e. ribD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus subtilis
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Bacillus subtilis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Escherichia coli
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P17618
gene ribG or ribD
-
Escherichia coli
P25539
gene ribD or ribG
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli to homogeneity
Bacillus subtilis
recombinant enzyme from Escherichia coli to homogeneity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
246735
Bacillus subtilis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
246735
Escherichia coli
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
domain structure, overview
Bacillus subtilis
More
domain structure, overview
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
37
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Bacillus subtilis
NADH
-
Escherichia coli
NADPH
-
Bacillus subtilis
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ribD, i.e. ribG, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Escherichia coli
gene ribG, i.e. ribD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Bacillus subtilis
NADH
-
Escherichia coli
NADPH
-
Bacillus subtilis
NADPH
-
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus subtilis
Mg2+
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Bacillus subtilis
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
Escherichia coli
-
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli to homogeneity
Bacillus subtilis
recombinant enzyme from Escherichia coli to homogeneity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
246735
Bacillus subtilis
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
246735
Escherichia coli
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
domain structure, overview
Bacillus subtilis
More
domain structure, overview
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
37
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
8
-
assay at
Escherichia coli
Other publictions for EC 1.1.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723878
Dawson
Structure of diaminohydroxypho ...
Acinetobacter baumannii
Acta Crystallogr. Sect. F
69
611-617
2013
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684754
Grill
Identification and characteriz ...
Streptomyces davawensis, Streptomyces davawensis 768
Arch. Microbiol.
188
377-387
2007
-
-
1
-
1
-
-
-
-
-
1
4
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246735
Richter
Biosynthesis of riboflavin: ch ...
Bacillus subtilis, Escherichia coli
J. Bacteriol.
179
2022-2028
1997
-
-
2
-
-
-
-
-
-
2
-
2
-
2
-
-
2
-
-
-
-
-
2
2
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
2
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
246736
Burrows
Presence in Escherichia coli o ...
Escherichia coli
J. Bacteriol.
136
657-667
1978
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-