Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 105% of initial activity | Geobacillus thermodenitrificans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity | Geobacillus thermodenitrificans | |
Ca2+ | 1 mM, no residual activity | Geobacillus thermodenitrificans | |
Co2+ | 1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity | Geobacillus thermodenitrificans | |
Cu2+ | 1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity | Geobacillus thermodenitrificans | |
K+ | 1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity | Geobacillus thermodenitrificans | |
Mg2+ | 1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity | Geobacillus thermodenitrificans | |
Mn2+ | 1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity | Geobacillus thermodenitrificans | |
Ni2+ | 1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity | Geobacillus thermodenitrificans | |
sodium dodecylsulfate | 1 mM, 3% residual activity; 1 mM, no residual activity | Geobacillus thermodenitrificans | |
Zn2+ | 1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
NADPH | cosubstrate octanal, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
1.15 | - |
octanal | cosubstrate NADPH, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
1.44 | - |
NAD+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
2.4 | - |
NADP+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
3.88 | - |
1-Octanol | cosubstrate NADP+, pH 8.0, 60°C | Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM, 136% of initial activity | Geobacillus thermodenitrificans | |
Fe2+ | 1 mM, 168% of initial activity | Geobacillus thermodenitrificans | |
additional information | enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases | Geobacillus thermodenitrificans | |
Na+ | 1 mM, 110% of initial activity | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41664 | - |
8 * 45000, SDS-PAGE, 8 * 41664, calculated | Geobacillus thermodenitrificans |
45000 | - |
8 * 45000, SDS-PAGE, 8 * 41664, calculated | Geobacillus thermodenitrificans |
90000 | - |
PAGE | Geobacillus thermodenitrificans |
380000 | - |
PAGE | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4IP64 | - |
- |
Geobacillus thermodenitrificans | A4ISB9 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A4IP64 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A4ISB9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-propanediol + 2 NAD+ + H2O | about 45% of the activity with ethanol | Geobacillus thermodenitrificans | ? + 2 NADH + 2 H+ | - |
r | |
1,3-propanediol + 2 NAD+ + H2O | about 5% of the activity with 1-octanol | Geobacillus thermodenitrificans | ? + 2 NADH + 2 H+ | - |
r | |
1,3-propanediol + 2 NAD+ + H2O | about 45% of the activity with ethanol | Geobacillus thermodenitrificans NG80-2 | ? + 2 NADH + 2 H+ | - |
r | |
1,3-propanediol + 2 NAD+ + H2O | about 5% of the activity with 1-octanol | Geobacillus thermodenitrificans NG80-2 | ? + 2 NADH + 2 H+ | - |
r | |
1-decanol + 2 NAD+ + H2O | about 20% of the activity with ethanol | Geobacillus thermodenitrificans | decanoic acid + 2 NADH + 2 H+ | - |
r | |
1-decanol + 2 NAD+ + H2O | about 50% of the activity with 1-octanol | Geobacillus thermodenitrificans | decanoic acid + 2 NADH + 2 H+ | - |
r | |
1-decanol + 2 NAD+ + H2O | about 20% of the activity with ethanol | Geobacillus thermodenitrificans NG80-2 | decanoic acid + 2 NADH + 2 H+ | - |
r | |
1-decanol + 2 NAD+ + H2O | about 50% of the activity with 1-octanol | Geobacillus thermodenitrificans NG80-2 | decanoic acid + 2 NADH + 2 H+ | - |
r | |
1-dodecanol + 2 NAD+ + H2O | about 17% of the activity with ethanol | Geobacillus thermodenitrificans | dodecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-dodecanol + 2 NAD+ + H2O | about 30% of the activity with 1-octanol | Geobacillus thermodenitrificans | dodecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-dodecanol + 2 NAD+ + H2O | about 17% of the activity with ethanol | Geobacillus thermodenitrificans NG80-2 | dodecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-dodecanol + 2 NAD+ + H2O | about 30% of the activity with 1-octanol | Geobacillus thermodenitrificans NG80-2 | dodecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-eicosanol + 2 NAD+ + H2O | about 25% of the activity with 1-octanol | Geobacillus thermodenitrificans | eicosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-eicosanol + 2 NAD+ + H2O | about 3% of the activity with ethanol | Geobacillus thermodenitrificans | eicosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-eicosanol + 2 NAD+ + H2O | about 3% of the activity with ethanol | Geobacillus thermodenitrificans NG80-2 | eicosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-eicosanol + 2 NAD+ + H2O | about 25% of the activity with 1-octanol | Geobacillus thermodenitrificans NG80-2 | eicosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-hexadecanol + 2 NAD+ + H2O | about 10% of the activity with ethanol | Geobacillus thermodenitrificans | hexadecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-hexadecanol + 2 NAD+ + H2O | about 25% of the activity with 1-octanol | Geobacillus thermodenitrificans | hexadecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octacosanol + 2 NAD+ + H2O | about 40% of the activity with ethanol | Geobacillus thermodenitrificans | octacosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octacosanol + 2 NAD+ + H2O | about 5% of the activity with 1-octanol | Geobacillus thermodenitrificans | octacosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octadecanol + 2 NAD+ + H2O | about 15% of the activity with 1-octanol | Geobacillus thermodenitrificans | octadecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octadecanol + 2 NAD+ + H2O | about 5% of the activity with ethanol | Geobacillus thermodenitrificans | octadecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octanol + 2 NAD+ + H2O | - |
Geobacillus thermodenitrificans | octanoic acid + 2 NADH + 2 H+ | - |
r | |
1-octanol + 2 NADP+ + H2O | - |
Geobacillus thermodenitrificans | octanoic acid + 2 NADPH + 2 H+ | - |
r | |
1-tetracosanol + 2 NAD+ + H2O | about 10% of the activity with 1-octanol | Geobacillus thermodenitrificans | tetracosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-tetracosanol + 2 NAD+ + H2O | about 30% of the activity with ethanol | Geobacillus thermodenitrificans | tetracosanoic acid + 2 NADH + 2 H+ | - |
r | |
1-tetradecanol + 2 NAD+ + H2O | about 15% of the activity with ethanol | Geobacillus thermodenitrificans | tetradecanoic acid + 2 NADH + 2 H+ | - |
r | |
1-tetradecanol + 2 NAD+ + H2O | about 30% of the activity with 1-octanol | Geobacillus thermodenitrificans | tetradecanoic acid + 2 NADH + 2 H+ | - |
r | |
decanal + NAD+ + H+ | about 10% of the activity with 1-octanol | Geobacillus thermodenitrificans | decanoic acid + NADH | - |
r | |
dodecanal + NAD+ + H+ | about 10% of the activity with 1-octanol | Geobacillus thermodenitrificans | dodecanoic acid + NADH | - |
r | |
glycerol + 2 NAD+ + H2O | about 45% of the activity with ethanol | Geobacillus thermodenitrificans | ? + 2 NADH + 2 H+ | - |
r | |
isopropanol + 2 NAD+ + H2O | about 15% of the activity with 1-octanol | Geobacillus thermodenitrificans | ? + 2 NADH + 2 H+ | - |
r | |
additional information | substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
octanal + NAD+ + H2O | - |
Geobacillus thermodenitrificans | octanoic acid + NADH + H+ | - |
r | |
octanal + NAD+ + H2O | - |
Geobacillus thermodenitrificans NG80-2 | octanoic acid + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 45000, SDS-PAGE, 8 * 41664, calculated | Geobacillus thermodenitrificans |
Synonyms | Comment | Organism |
---|---|---|
ADH1 | - |
Geobacillus thermodenitrificans |
ADH2 | - |
Geobacillus thermodenitrificans |
long-chain alkyl alcohol dehydrogenase | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
14 h, more than 50% of initial activity | Geobacillus thermodenitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.5 | - |
NAD+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
283.1 | - |
NADP+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
4808 | - |
NADPH | cosubstrate octanal, pH 8.0, 60°C | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ is prefered over NADP+ | Geobacillus thermodenitrificans | |
NAD+ | NADP+ is prefered over NAD+ | Geobacillus thermodenitrificans | |
NADH | - |
Geobacillus thermodenitrificans | |
NADP+ | NAD+ is prefered over NADP+ | Geobacillus thermodenitrificans | |
NADP+ | NADP+ is prefered over NAD+ | Geobacillus thermodenitrificans | |
NADPH | - |
Geobacillus thermodenitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.7 | - |
NAD+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
67.2 | - |
1-Octanol | cosubstrate NADP+, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
117.9 | - |
NADP+ | cosubstrate 1-octanol, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
450.8 | - |
octanal | cosubstrate NADPH, pH 8.0, 60°C | Geobacillus thermodenitrificans | |
5724 | - |
NADPH | cosubstrate octanal, pH 8.0, 60°C | Geobacillus thermodenitrificans |