BRENDA - Enzyme Database show
show all sequences of 1.1.1.192

Long-chain n-alkanol dehydrogenase from Pseudomonas putida

Nagashima, H.; Inoue, J.; Sasaki, E.; Yamamoto, S.; Sasaki, Y.; Yamauchi-Inomata, Y.; Harayama, S.; J. Ferment. Bioeng. 82, 328-333 (1996)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
n-butanol
-
Pseudomonas putida
0.053
-
n-Propanol
-
Pseudomonas putida
0.095
-
n-decanal
-
Pseudomonas putida
0.14
-
n-Hexanol
-
Pseudomonas putida
0.17
-
NADH
-
Pseudomonas putida
0.18
-
n-Pentanol
-
Pseudomonas putida
0.23
-
n-decanol
-
Pseudomonas putida
0.26
-
n-Heptanol
-
Pseudomonas putida
0.31
-
NAD+
-
Pseudomonas putida
0.34
-
n-Octanol
-
Pseudomonas putida
0.36
-
n-nonanol
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zinc
1.1 mol per mol of subunit
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Pseudomonas putida
163000
-
gel filtration
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
long-chain alcohol + NAD+ + H2O
long chain n-alcanols up to C12
347799
Pseudomonas putida
long-chain carboxylate + NADH
-
-
-
r
n-butanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-butanal + NADH
-
-
-
?
n-decanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-decanal + NADH
-
-
-
r
n-heptanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-heptanal + NADH
-
-
-
?
n-hexanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-hexanal + NADH
-
-
-
?
n-nonanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-nonanal + NADH
-
-
-
?
n-octanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-octanal + NADH
-
-
-
?
n-pentanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-pentanal + NADH
-
-
-
?
n-propanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-propanal + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
N-terminal sequence
Pseudomonas putida
tetramer
4 * 40000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas putida
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to
Pseudomonas putida
70
-
10 min, all activity lost
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
n-decanol, reduction of
Pseudomonas putida
9.5
-
n-decanol, oxidation of
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
absolute requirement
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
absolute requirement
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
n-butanol
-
Pseudomonas putida
0.053
-
n-Propanol
-
Pseudomonas putida
0.095
-
n-decanal
-
Pseudomonas putida
0.14
-
n-Hexanol
-
Pseudomonas putida
0.17
-
NADH
-
Pseudomonas putida
0.18
-
n-Pentanol
-
Pseudomonas putida
0.23
-
n-decanol
-
Pseudomonas putida
0.26
-
n-Heptanol
-
Pseudomonas putida
0.31
-
NAD+
-
Pseudomonas putida
0.34
-
n-Octanol
-
Pseudomonas putida
0.36
-
n-nonanol
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zinc
1.1 mol per mol of subunit
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Pseudomonas putida
163000
-
gel filtration
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
long-chain alcohol + NAD+ + H2O
long chain n-alcanols up to C12
347799
Pseudomonas putida
long-chain carboxylate + NADH
-
-
-
r
n-butanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-butanal + NADH
-
-
-
?
n-decanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-decanal + NADH
-
-
-
r
n-heptanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-heptanal + NADH
-
-
-
?
n-hexanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-hexanal + NADH
-
-
-
?
n-nonanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-nonanal + NADH
-
-
-
?
n-octanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-octanal + NADH
-
-
-
?
n-pentanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-pentanal + NADH
-
-
-
?
n-propanol + NAD+ + H2O
-
347799
Pseudomonas putida
n-propanal + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
N-terminal sequence
Pseudomonas putida
tetramer
4 * 40000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Pseudomonas putida
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
stable up to
Pseudomonas putida
70
-
10 min, all activity lost
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
n-decanol, reduction of
Pseudomonas putida
9.5
-
n-decanol, oxidation of
Pseudomonas putida
Other publictions for EC 1.1.1.192
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712962
Liu
Two novel metal-independent lo ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
155
2078-2085
2009
1
-
1
-
-
-
10
5
-
3
4
-
-
4
-
-
-
-
-
-
-
-
26
1
1
-
1
3
1
1
-
4
-
-
-
1
-
2
6
-
-
-
-
19
-
5
-
5
4
-
-
-
-
-
-
-
-
-
26
1
2
-
2
3
2
2
-
-
-
-
-
-
5
5
656603
Luesch
Biosynthesis of 4-methylprolin ...
Nostoc sp.
J. Org. Chem.
68
83-91
2003
-
-
1
-
-
-
-
2
-
1
1
1
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347799
Nagashima
-
Long-chain n-alkanol dehydroge ...
Pseudomonas putida
J. Ferment. Bioeng.
82
328-333
1996
-
-
-
-
-
-
-
11
-
1
2
-
-
1
-
-
1
-
-
-
-
-
9
2
1
-
2
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
1
2
-
-
-
-
1
-
-
-
-
9
2
1
-
2
-
2
-
-
-
-
-
-
-
-
-
288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
-
-
-
-
-
-
5
7
-
1
2
1
-
7
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
7
-
1
2
1
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347798
Ribas de Pouplana
-
Structural properties of long- ...
Equus caballus
Biochem. J.
276
433-438
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347795
Ueda
Long-chain alcohol dehydrogena ...
Candida tropicalis, Yarrowia lipolytica
Methods Enzymol.
188
171-175
1990
-
-
-
-
-
6
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347796
Yamada
Subcellular localization of lo ...
Candida tropicalis
Arch. Microbiol.
128
145-151
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347794
Lee
Characterization of fatty alco ...
Rattus norvegicus
J. Biol. Chem.
254
2892-2896
1979
1
-
-
-
-
-
3
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347800
Eklund
Three-dimensional structure of ...
Equus caballus
J. Mol. Biol.
102
27-59
1976
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-