BRENDA - Enzyme Database show
show all sequences of 1.1.1.192

NAD-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from Amycolatopsis methanolica

Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.; Eur. J. Biochem. 206, 511-518 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Amycolatopsis methanolica
CuCl2
-
Amycolatopsis methanolica
HgCl2
-
Amycolatopsis methanolica
KCN
-
Amycolatopsis methanolica
additional information
inhibition differs for ethanol and formaldehyde as substrates
Amycolatopsis methanolica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
1-Octanol
-
Amycolatopsis methanolica
1.7
-
12-Hydroxydodecanoic acid
-
Amycolatopsis methanolica
3.4
-
1-Hexanol
-
Amycolatopsis methanolica
26.5
-
1-Pentanol
-
Amycolatopsis methanolica
84
-
1-butanol
-
Amycolatopsis methanolica
324
-
1-propanol
-
Amycolatopsis methanolica
343
-
ethanol
-
Amycolatopsis methanolica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zinc
6.1 atoms/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
117000
120000
PAGE, gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
long-chain alcohol + NAD+ + H2O
Amycolatopsis methanolica
enzyme also uses formaldehyde and short chain aliphatic alcohols
long-chain carboxylate + NADH
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Amycolatopsis methanolica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12-hydroxydodecanoic acid + NAD+ + H2O
-
288324
Amycolatopsis methanolica
? + NADH
-
-
-
?
ethanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
ethanal + NADH
-
-
-
?
long-chain alcohol + NAD+ + H2O
enzyme also uses formaldehyde and short chain aliphatic alcohols
288324
Amycolatopsis methanolica
long-chain carboxylate + NADH
-
-
-
-
n-butanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-butanal + NADH
-
-
-
-
n-hexanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-hexanal + NADH
-
-
-
-
n-pentanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-pentanal + NADH
-
-
-
-
n-propanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-propanal + NADH
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
N-terminal sequence
Amycolatopsis methanolica
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Amycolatopsis methanolica
CuCl2
-
Amycolatopsis methanolica
HgCl2
-
Amycolatopsis methanolica
KCN
-
Amycolatopsis methanolica
additional information
inhibition differs for ethanol and formaldehyde as substrates
Amycolatopsis methanolica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
1-Octanol
-
Amycolatopsis methanolica
1.7
-
12-Hydroxydodecanoic acid
-
Amycolatopsis methanolica
3.4
-
1-Hexanol
-
Amycolatopsis methanolica
26.5
-
1-Pentanol
-
Amycolatopsis methanolica
84
-
1-butanol
-
Amycolatopsis methanolica
324
-
1-propanol
-
Amycolatopsis methanolica
343
-
ethanol
-
Amycolatopsis methanolica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zinc
6.1 atoms/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
117000
120000
PAGE, gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
long-chain alcohol + NAD+ + H2O
Amycolatopsis methanolica
enzyme also uses formaldehyde and short chain aliphatic alcohols
long-chain carboxylate + NADH
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
12-hydroxydodecanoic acid + NAD+ + H2O
-
288324
Amycolatopsis methanolica
? + NADH
-
-
-
?
ethanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
ethanal + NADH
-
-
-
?
long-chain alcohol + NAD+ + H2O
enzyme also uses formaldehyde and short chain aliphatic alcohols
288324
Amycolatopsis methanolica
long-chain carboxylate + NADH
-
-
-
-
n-butanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-butanal + NADH
-
-
-
-
n-hexanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-hexanal + NADH
-
-
-
-
n-pentanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-pentanal + NADH
-
-
-
-
n-propanol + NAD+ + H2O
-
288324
Amycolatopsis methanolica
n-propanal + NADH
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
N-terminal sequence
Amycolatopsis methanolica
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
Other publictions for EC 1.1.1.192
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712962
Liu
Two novel metal-independent lo ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
155
2078-2085
2009
1
-
1
-
-
-
10
5
-
3
4
-
-
4
-
-
-
-
-
-
-
-
26
1
1
-
1
3
1
1
-
4
-
-
-
1
-
2
6
-
-
-
-
19
-
5
-
5
4
-
-
-
-
-
-
-
-
-
26
1
2
-
2
3
2
2
-
-
-
-
-
-
5
5
656603
Luesch
Biosynthesis of 4-methylprolin ...
Nostoc sp.
J. Org. Chem.
68
83-91
2003
-
-
1
-
-
-
-
2
-
1
1
1
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
347799
Nagashima
-
Long-chain n-alkanol dehydroge ...
Pseudomonas putida
J. Ferment. Bioeng.
82
328-333
1996
-
-
-
-
-
-
-
11
-
1
2
-
-
1
-
-
1
-
-
-
-
-
9
2
1
-
2
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
1
2
-
-
-
-
1
-
-
-
-
9
2
1
-
2
-
2
-
-
-
-
-
-
-
-
-
288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
-
-
-
-
-
-
5
7
-
1
2
1
-
7
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
7
-
1
2
1
-
-
-
-
-
-
-
-
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347798
Ribas de Pouplana
-
Structural properties of long- ...
Equus caballus
Biochem. J.
276
433-438
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347795
Ueda
Long-chain alcohol dehydrogena ...
Candida tropicalis, Yarrowia lipolytica
Methods Enzymol.
188
171-175
1990
-
-
-
-
-
6
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
9
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347796
Yamada
Subcellular localization of lo ...
Candida tropicalis
Arch. Microbiol.
128
145-151
1980
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347794
Lee
Characterization of fatty alco ...
Rattus norvegicus
J. Biol. Chem.
254
2892-2896
1979
1
-
-
-
-
-
3
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
1
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347800
Eklund
Three-dimensional structure of ...
Equus caballus
J. Mol. Biol.
102
27-59
1976
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-