BRENDA - Enzyme Database show
show all sequences of 1.1.1.19

An overview of gamma-hydroxybutyrate catabolism: the role of the cytosolic NADP+-dependent oxidoreductase EC 1.1.1.19 and of a mitochondrial hydroxyacid-oxoacid transhydrogenase in the initial, rate-limiting step in this pathway

Kaufman, E.E.; Nelson, T.; Neurochem. Res. 16, 965-974 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Mesocricetus auratus
2-mercaptoethanol
preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect
Rattus norvegicus
acetoacetate
-
Mesocricetus auratus
acetoacetate
-
Rattus norvegicus
alpha-ketoglutarate
-
Mesocricetus auratus
alpha-ketoglutarate
-
Rattus norvegicus
branched alpha-ketoacids
-
Mesocricetus auratus
branched alpha-ketoacids
-
Rattus norvegicus
dithiothreitol
-
Mesocricetus auratus
dithiothreitol
preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect
Rattus norvegicus
DL-beta-hydroxybutyrate
-
Mesocricetus auratus
DL-beta-hydroxybutyrate
-
Rattus norvegicus
phenylacetate
-
Mesocricetus auratus
phenylacetate
-
Rattus norvegicus
Salicylates
-
Mesocricetus auratus
Salicylates
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00045
-
gamma-hydroxybutyrate
coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction, brain and kidney
Rattus norvegicus
0.0022
-
gamma-hydroxybutyrate
kidney
Rattus norvegicus
0.0025
-
gamma-hydroxybutyrate
brain
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucuronate + NADPH
Homo sapiens
-
L-gulonate + NADP+
-
-
r
D-glucuronate + NADPH
Rattus norvegicus
-
L-gulonate + NADP+
-
-
r
gamma-hydroxybutyrate + NADP+
Mesocricetus auratus
preverably in a coupled reaction in vivo with reduction of D-glucuronate
succinic semialdehyde + NADPH
-
-
?
L-gulonate + NADP+
Homo sapiens
-
D-glucuronate + NADPH
-
-
r
L-gulonate + NADP+
Rattus norvegicus
-
D-glucuronate + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Mesocricetus auratus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mesocricetus auratus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
kidney
-
Homo sapiens
-
liver
-
Mesocricetus auratus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucuronate + NADPH
-
286147
Homo sapiens
L-gulonate + NADP+
-
-
-
r
D-glucuronate + NADPH
-
286147
Rattus norvegicus
L-gulonate + NADP+
-
-
-
r
gamma-hydroxybutyrate + NADP+
-
286147
Mesocricetus auratus
succinic semialdehyde + NADPH
-
-
-
?
gamma-hydroxybutyrate + NADP+
preverably in a coupled reaction in vivo with reduction of D-glucuronate
286147
Mesocricetus auratus
succinic semialdehyde + NADPH
-
-
-
?
L-gulonate + NADP+
-
286147
Homo sapiens
D-glucuronate + NADPH
-
-
-
r
L-gulonate + NADP+
-
286147
Rattus norvegicus
D-glucuronate + NADPH
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
shifted pH-optimum for the coupled reaction of oxidation of gamma-hydroxybutyrate and reduction of D-glucuronate in vitro
Mesocricetus auratus
7.5
-
in vivo for coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction
Mesocricetus auratus
7.5
-
-
Rattus norvegicus
9
-
substrate gamma-hydroxybutyrate, at pH 7.0 activity is reduced by 50%
Mesocricetus auratus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Homo sapiens
7
10
-
Mesocricetus auratus
7
10
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Mesocricetus auratus
NADP+
-
Rattus norvegicus
NADP+
-
Homo sapiens
NADPH
-
Mesocricetus auratus
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Mesocricetus auratus
NADP+
-
Rattus norvegicus
NADP+
-
Homo sapiens
NADPH
-
Mesocricetus auratus
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Mesocricetus auratus
2-mercaptoethanol
preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect
Rattus norvegicus
acetoacetate
-
Mesocricetus auratus
acetoacetate
-
Rattus norvegicus
alpha-ketoglutarate
-
Mesocricetus auratus
alpha-ketoglutarate
-
Rattus norvegicus
branched alpha-ketoacids
-
Mesocricetus auratus
branched alpha-ketoacids
-
Rattus norvegicus
dithiothreitol
-
Mesocricetus auratus
dithiothreitol
preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect
Rattus norvegicus
DL-beta-hydroxybutyrate
-
Mesocricetus auratus
DL-beta-hydroxybutyrate
-
Rattus norvegicus
phenylacetate
-
Mesocricetus auratus
phenylacetate
-
Rattus norvegicus
Salicylates
-
Mesocricetus auratus
Salicylates
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00045
-
gamma-hydroxybutyrate
coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction, brain and kidney
Rattus norvegicus
0.0022
-
gamma-hydroxybutyrate
kidney
Rattus norvegicus
0.0025
-
gamma-hydroxybutyrate
brain
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glucuronate + NADPH
Homo sapiens
-
L-gulonate + NADP+
-
-
r
D-glucuronate + NADPH
Rattus norvegicus
-
L-gulonate + NADP+
-
-
r
gamma-hydroxybutyrate + NADP+
Mesocricetus auratus
preverably in a coupled reaction in vivo with reduction of D-glucuronate
succinic semialdehyde + NADPH
-
-
?
L-gulonate + NADP+
Homo sapiens
-
D-glucuronate + NADPH
-
-
r
L-gulonate + NADP+
Rattus norvegicus
-
D-glucuronate + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mesocricetus auratus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
kidney
-
Homo sapiens
-
liver
-
Mesocricetus auratus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucuronate + NADPH
-
286147
Homo sapiens
L-gulonate + NADP+
-
-
-
r
D-glucuronate + NADPH
-
286147
Rattus norvegicus
L-gulonate + NADP+
-
-
-
r
gamma-hydroxybutyrate + NADP+
-
286147
Mesocricetus auratus
succinic semialdehyde + NADPH
-
-
-
?
gamma-hydroxybutyrate + NADP+
preverably in a coupled reaction in vivo with reduction of D-glucuronate
286147
Mesocricetus auratus
succinic semialdehyde + NADPH
-
-
-
?
L-gulonate + NADP+
-
286147
Homo sapiens
D-glucuronate + NADPH
-
-
-
r
L-gulonate + NADP+
-
286147
Rattus norvegicus
D-glucuronate + NADPH
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
shifted pH-optimum for the coupled reaction of oxidation of gamma-hydroxybutyrate and reduction of D-glucuronate in vitro
Mesocricetus auratus
7.5
-
in vivo for coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction
Mesocricetus auratus
7.5
-
-
Rattus norvegicus
9
-
substrate gamma-hydroxybutyrate, at pH 7.0 activity is reduced by 50%
Mesocricetus auratus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Homo sapiens
7
10
-
Mesocricetus auratus
7
10
-
Rattus norvegicus
Other publictions for EC 1.1.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711916
Sahoo
Synthesis and biological evalu ...
Rattus norvegicus
Eur. J. Med. Chem.
45
909-914
2010
-
-
-
-
-
-
1
-
-
-
-
1
-
1
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1
-
1
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1
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1
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1
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1
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1
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-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686647
Linster
Vitamin C. Biosynthesis, recyc ...
Homo sapiens, Rattus norvegicus
FEBS J.
274
1-22
2007
-
-
-
-
-
-
2
2
2
-
-
4
-
2
-
-
-
-
-
2
-
-
5
2
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
2
-
2
2
-
-
4
-
-
-
-
-
2
-
-
5
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667168
Barski
Developmental expression and f ...
Homo sapiens, Mus musculus
Am. J. Physiol. Renal Physiol.
289
F200-F207
2005
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286150
Shinoda
Inhibition of human aldehyde r ...
Homo sapiens
Biol. Pharm. Bull.
22
741-744
1999
-
-
-
-
-
-
5
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286146
Goode
Accumulation of xylitol in the ...
Bos taurus, Homo sapiens, Rattus norvegicus
FEBS Lett.
395
174-178
1996
-
-
-
-
-
-
6
2
-
-
4
6
-
6
-
-
2
-
-
3
2
-
6
2
2
-
-
-
2
-
2
6
-
-
-
-
-
-
6
-
-
-
-
6
-
2
-
-
4
6
-
-
-
2
-
3
2
-
6
2
2
-
-
-
2
-
2
-
-
-
-
-
-
-
286148
Sato
Human kidney aldose and aldehy ...
Canis lupus familiaris, Homo sapiens, Rattus norvegicus
J. Diabetes Complicat.
7
179-187
1993
-
-
-
-
-
-
9
6
-
-
2
4
-
3
-
-
1
-
-
3
1
-
6
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
9
-
6
-
-
2
4
-
-
-
1
-
3
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286147
Kaufman
An overview of gamma-hydroxybu ...
Homo sapiens, Mesocricetus auratus, Rattus norvegicus
Neurochem. Res.
16
965-974
1991
-
-
-
-
-
-
16
3
-
-
-
5
-
3
-
-
1
-
-
7
-
-
6
-
-
-
-
-
4
3
-
6
-
-
-
-
-
-
6
-
-
-
-
16
-
3
-
-
-
5
-
-
-
1
-
7
-
-
6
-
-
-
-
-
4
3
-
-
-
-
-
-
-
-
286149
Bhatnagar
Human liver aldehyde reductase ...
Homo sapiens
Arch. Biochem. Biophys.
287
329-336
1991
-
-
-
-
-
-
3
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
1
1
1
1
2
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
286145
Poulsom
Inhibition of hexonate dehydro ...
Bos taurus
Biochem. Pharmacol.
35
2955-2959
1986
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286139
Flynn
Aldehyde reductase (L-hexonate ...
Sus scrofa
Methods Enzymol.
89
501-506
1982
-
-
-
-
-
-
9
7
-
-
2
-
-
1
-
-
1
-
-
1
1
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
9
-
7
-
-
2
-
-
-
-
1
-
1
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286142
Kaufman
Kinetics of coupled gamma-hydr ...
Mesocricetus auratus
J. Biol. Chem.
256
6890-6894
1981
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286143
Tulsiani
Resolution and partial charact ...
Mus musculus
J. Biol. Chem.
252
2545-2550
1977
-
-
-
-
-
-
6
13
-
-
2
1
-
2
-
-
1
-
-
1
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
13
-
-
2
1
-
-
-
1
-
1
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286140
Bublitz
-
Ascorbic acid synthesis in ani ...
Sus scrofa
Methods Enzymol.
6
334-337
1963
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286141
York
TPN-L-gulonate dehydrogenase ...
Sus scrofa
Biochim. Biophys. Acta
47
298-306
1961
-
-
-
-
-
2
1
2
-
-
-
-
2
1
-
-
1
-
-
1
-
1
3
-
-
-
2
-
1
-
1
2
-
-
-
-
-
-
2
-
-
2
-
1
-
2
-
-
-
-
2
-
-
1
-
1
-
1
3
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
286144
Sivak
Enzymes of meso-inositol catab ...
Schwanniomyces occidentalis
Biochim. Biophys. Acta
53
426-428
1961
-
-
-
-
-
-
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