Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Mesocricetus auratus | |
2-mercaptoethanol | preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect | Rattus norvegicus | |
acetoacetate | - |
Mesocricetus auratus | |
acetoacetate | - |
Rattus norvegicus | |
alpha-ketoglutarate | - |
Mesocricetus auratus | |
alpha-ketoglutarate | - |
Rattus norvegicus | |
branched alpha-ketoacids | - |
Mesocricetus auratus | |
branched alpha-ketoacids | - |
Rattus norvegicus | |
dithiothreitol | - |
Mesocricetus auratus | |
dithiothreitol | preincubation of gamma-hydroxybutyrate reductase revealed no inhibitory effect | Rattus norvegicus | |
DL-beta-hydroxybutyrate | - |
Mesocricetus auratus | |
DL-beta-hydroxybutyrate | - |
Rattus norvegicus | |
phenylacetate | - |
Mesocricetus auratus | |
phenylacetate | - |
Rattus norvegicus | |
Salicylates | - |
Mesocricetus auratus | |
Salicylates | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
gamma-hydroxybutyrate | coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction, brain and kidney | Rattus norvegicus | |
0.00045 | - |
gamma-hydroxybutyrate | enzyme from brain, D-glucuronate reduction-coupled assay | Rattus norvegicus | |
0.0022 | - |
gamma-hydroxybutyrate | kidney | Rattus norvegicus | |
0.0022 | - |
gamma-hydroxybutyrate | enzyme from kidney, uncoupled assay | Rattus norvegicus | |
0.0025 | - |
gamma-hydroxybutyrate | brain | Rattus norvegicus | |
0.0025 | - |
gamma-hydroxybutyrate | enzyme from brain, uncoupled assay | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucuronate + NADPH | Homo sapiens | - |
L-gulonate + NADP+ | - |
r | |
D-glucuronate + NADPH | Rattus norvegicus | - |
L-gulonate + NADP+ | - |
r | |
gamma-hydroxybutyrate + NADP+ | Mesocricetus auratus | preverably in a coupled reaction in vivo with reduction of D-glucuronate | succinic semialdehyde + NADPH | - |
? | |
L-gulonate + NADP+ | Homo sapiens | - |
D-glucuronate + NADPH | - |
r | |
L-gulonate + NADP+ | Rattus norvegicus | - |
D-glucuronate + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mesocricetus auratus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Mesocricetus auratus | - |
muscle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucuronate + NADPH | - |
Homo sapiens | L-gulonate + NADP+ | - |
r | |
D-glucuronate + NADPH | - |
Rattus norvegicus | L-gulonate + NADP+ | - |
r | |
gamma-hydroxybutyrate + NADP+ | - |
Mesocricetus auratus | succinic semialdehyde + NADPH | - |
? | |
gamma-hydroxybutyrate + NADP+ | preverably in a coupled reaction in vivo with reduction of D-glucuronate | Mesocricetus auratus | succinic semialdehyde + NADPH | - |
? | |
gamma-hydroxybutyrate + NADP+ | - |
Rattus norvegicus | succinic semialdehyde + NADPH + H+ | - |
r | |
L-gulonate + NADP+ | - |
Homo sapiens | D-glucuronate + NADPH | - |
r | |
L-gulonate + NADP+ | - |
Rattus norvegicus | D-glucuronate + NADPH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
shifted pH-optimum for the coupled reaction of oxidation of gamma-hydroxybutyrate and reduction of D-glucuronate in vitro | Mesocricetus auratus |
7.5 | - |
- |
Rattus norvegicus |
7.5 | - |
in vivo for coupled reaction of gamma-hydroxybutyrate oxidation and D-glucuronate reduction | Mesocricetus auratus |
9 | - |
substrate gamma-hydroxybutyrate, at pH 7.0 activity is reduced by 50% | Mesocricetus auratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Homo sapiens |
7 | 10 | - |
Rattus norvegicus |
7 | 10 | - |
Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Mesocricetus auratus | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Mesocricetus auratus |