Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.188 extracted from

  • Kabututu, Z.; Manin, M.; Pointud, J.C.; Maruyama, T.; Nagata, N.; Lambert, S.; Lefrancois-Martinez, A.M.; Martinez, A.; Urade, Y.
    Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7 (2009), J. Biochem., 145, 161-168.
    View publication on PubMed
Search for more literature for 1.1.1.188

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-28a and expressed in Escherichia coli BL21 (DE3) Mus musculus
into vector pET-28a and expressed in Escherichia coli BL21 (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information heat treatment of AKR1B1 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activity, which is strictly dependent on the presence of NADPH Homo sapiens
additional information heat treatment of AKR1B3 and AKR1B7 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activities, which is strictly dependent on the presence of NADPH Mus musculus
sorbinil
-
 Homo sapiens
sorbinil significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor Mus musculus
Tolrestat
-
 Homo sapiens
Tolrestat significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 prostaglandin H2
-
 Homo sapiens
0.0038
-
 prostaglandin H2 AKR1B7 Mus musculus
0.0057
-
 NADPH
-
 Homo sapiens
0.0074
-
 NADPH AKR1B3 Mus musculus
0.0092
-
 NADPH AKR1B7 Mus musculus
0.0093
-
 prostaglandin H2 AKR1B3 Mus musculus
0.018
-
 prostaglandin H2
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Mus musculus
35000
-
 x * 35000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P15121
-
-
Homo sapiens P42330
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity, on Ni-NTA resin and by gel filtration Mus musculus
to apparent homogeneity, on Ni-NTA resin and by gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney AKR1B3 Mus musculus
-
lung
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prostaglandin H2 + NADPH + H+ aldo-keto reductase has prostaglandin F2alpha synthase activity. AKR1B1 is a more efficient prostaglandin F2alpha synthase than AKR1C3 Homo sapiens (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
prostaglandin H2 + NADPH + H+ aldo-keto reductases AKR1B3 and AKR1B7 have prostaglandin F2alpha synthase activity Mus musculus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Mus musculus
? x * 35000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
AKR1B1
-
 Homo sapiens
AKR1B3
-
 Mus musculus
AKR1B7
-
 Mus musculus
AKR1C3
-
 Homo sapiens
aldo-keto reductase
-
 Mus musculus
aldo-keto reductase
-
 Homo sapiens
PGFS
-
 Mus musculus
PGFS
-
 Homo sapiens
prostaglandin F2alpha synthase
-
 Mus musculus
prostaglandin F2alpha synthase
-
 Homo sapiens
vas deferens protein
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mus musculus
NADPH
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00026
-
 Tolrestat AKR1B3 Mus musculus
0.00089
-
 sorbinil AKR1B3 Mus musculus
0.0036
-
 Tolrestat
-
 Homo sapiens
0.0217
-
 sorbinil
-
 Homo sapiens