BRENDA - Enzyme Database show
show all sequences of 1.1.1.188

Crystallization and preliminary X-ray crystallographic studies of Trypanosoma brucei prostaglandin F2 alpha synthase

Okano, Y.; Inoue, T.; Kubata, B.K.; Kabututu, Z.; Urade, Y.; Matsumura, H.; Kai, Y.; J. Biochem. 132, 859-861 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3) as GST-fusion protein
Trypanosoma brucei
Crystallization (Commentary)
Crystallization
Organism
10 mg/ml purified recombinant enzyme from 0.1 M HEPES/NaOH, pH 7.5, 2.0 M ammonium sulfate, 5% w/v PEG 400, and 0.5 M NADPH by hanging drop vapour diffusion method, 20°C, 3 weeks, X-ray diffraction structure determination at room temperature and analysis at 2.6 A resolution
Trypanosoma brucei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Trypanosoma brucei
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin H2 + NADPH
Trypanosoma brucei
-
prostaglandin F2alpha + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography
Trypanosoma brucei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9,10-phenanthrenequinone + NADPH
-
655915
Trypanosoma brucei
?
-
-
-
?
additional information
enzyme does not reduce prostaglandins D2 and E2
655915
Trypanosoma brucei
?
-
-
-
-
prostaglandin H2 + NADPH
-
655915
Trypanosoma brucei
prostaglandin F2alpha + NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Trypanosoma brucei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Trypanosoma brucei
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Trypanosoma brucei
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3) as GST-fusion protein
Trypanosoma brucei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Trypanosoma brucei
Crystallization (Commentary) (protein specific)
Crystallization
Organism
10 mg/ml purified recombinant enzyme from 0.1 M HEPES/NaOH, pH 7.5, 2.0 M ammonium sulfate, 5% w/v PEG 400, and 0.5 M NADPH by hanging drop vapour diffusion method, 20°C, 3 weeks, X-ray diffraction structure determination at room temperature and analysis at 2.6 A resolution
Trypanosoma brucei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Trypanosoma brucei
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prostaglandin H2 + NADPH
Trypanosoma brucei
-
prostaglandin F2alpha + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
9,10-phenanthrenequinone + NADPH
-
655915
Trypanosoma brucei
?
-
-
-
?
additional information
enzyme does not reduce prostaglandins D2 and E2
655915
Trypanosoma brucei
?
-
-
-
-
prostaglandin H2 + NADPH
-
655915
Trypanosoma brucei
prostaglandin F2alpha + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Trypanosoma brucei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Trypanosoma brucei
Other publictions for EC 1.1.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724504
Gram
Biosynthesis and degradation o ...
Canis lupus familiaris
Biol. Reprod.
89
002
2013
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725674
Liedtke
Development of potent and sele ...
Homo sapiens
J. Med. Chem.
56
2429-2446
2013
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1
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726469
Siemieniuch
Prostaglandin endoperoxide syn ...
Felis catus
Reprod. Domest. Anim.
48
72-78
2013
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1
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1
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725083
Bresson
The prostaglandin F synthase a ...
Homo sapiens
Front. Pharmacol.
3
98
2012
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1
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1
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707934
Yoshikawa
Preferential localization of p ...
Mus musculus
Brain Res.
1367
22-32
2011
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713047
Phillips
Genes for prostaglandin synthe ...
Homo sapiens
Mol. Hum. Reprod.
17
1-13
2011
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724961
Nagata
Catalytic mechanism of the pri ...
Homo sapiens, Mus musculus
FEBS J.
278
1288-1298
2011
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7
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725608
Bresson
The human aldose reductase AKR ...
Homo sapiens
J. Clin. Endocrinol. Metab.
96
210-219
2011
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712494
Fujimori
Suppression of adipocyte diffe ...
Mus musculus
J. Biol. Chem.
285
8880-8886
2010
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1
1
1
1
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697271
Byrns
Type 5 17beta-hydroxysteroid d ...
Homo sapiens
Chem. Biol. Interact.
178
221-227
2009
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1
1
1
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19
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34
36
17
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704349
Kabututu
Prostaglandin F2alpha synthase ...
Homo sapiens, Mus musculus
J. Biochem.
145
161-168
2009
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2
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6
7
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2
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9
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2
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2
4
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3
3
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8
4
7
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9
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3
3
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705002
Suzuki-Yamamoto
Colocalization of prostaglandi ...
Rattus norvegicus
J. Lipid Res.
50
1996-2003
2009
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706695
Wasielak
Effect of the conceptus on ute ...
Sus scrofa
Reprod. Fertil. Dev.
21
709-717
2009
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684436
Kowalewski
Canine prostaglandin F2alpha r ...
Canis lupus familiaris
Anim. Reprod. Sci.
107
161-175
2008
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685035
Byrns
An indomethacin analogue, N-(4 ...
Homo sapiens
Biochem. Pharmacol.
75
484-493
2008
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1
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10
2
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10
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687797
Moriuchi
Molecular characterization of ...
Mus musculus, Sus scrofa
J. Biol. Chem.
283
792-801
2008
1
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24
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24
2
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1
689092
Wasielak
The influence of embryo presen ...
Sus scrofa
Mol. Reprod. Dev.
75
1208-1216
2008
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690009
Dozier
Two pathways for prostaglandin ...
Homo sapiens, Homo sapiens AKR1C3, Macaca fascicularis, Macaca fascicularis AKR1C3
Reproduction
136
53-63
2008
1
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699917
Inagaki
Human liver dihydrodiol dehydr ...
Homo sapiens
J. Sep. Sci.
31
735-740
2008
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687013
Suzuki-Yamamoto
Co-localization of prostagland ...
Mus musculus
Histochem. Cell Biol.
128
317-322
2007
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688008
Waclawik
Differential expression of pro ...
Sus scrofa
J. Endocrinol.
194
499-510
2007
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661605
Waclawik
Molecular cloning and spatiote ...
Sus scrofa
Endocrinology
147
210-221
2006
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12
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1
1
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Crystal structure of human pro ...
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Coupling between cyclooxygenas ...
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657211
Watanabe
Prostaglandin F synthase ...
Bos taurus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Rattus norvegicus, Trypanosoma brucei
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Endo
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Detection and regulation of th ...
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cDNA cloning, expression, and ...
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Down-regulation of oxytocin-in ...
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Biol. Reprod.
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A lung type prostaglandin F sy ...
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Purification and characterizat ...
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Watanabe
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Watanabe
Structural similarity of bovin ...
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Content and formation of prost ...
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Enzymatic formation of prostag ...
Bos taurus
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7035-7041
1985
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389461
Wong
Purification of PGD2 11-ketore ...
Oryctolagus cuniculus
Methods Enzymol.
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117-125
1982
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389462
Wong
Purification and partial chara ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
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169-178
1981
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389463
Reingold
A novel prostaglandin 11-keto ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
659
179-188
1981
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389464
Watanabe
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Enzymic conversion of prostagl ...
Rattus norvegicus
Biochem. Int.
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603-610
1981
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