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Literature summary for 1.1.1.188 extracted from

  • Suzuki-Yamamoto, T.; Fujii, Y.; Miyano, M.; Chen, L.Y.; Takahashi, T.; Yokoi, H.; Tsuruo, Y.; Ishimura, K.; Watanabe, K.
    cDNA cloning and mutagenesis study of liver-type prostaglandin F synthase, and identification of the prostaglandin F producing cells in the liver (2002), Adv. Exp. Med. Biol., 507, 263-268.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
liver-type isozyme, full length cDNA from cDNA library, DNA and amino acid sequence determination and analysis, transient expression of wild-type and mutant enzymes in COS-7 cells Bos taurus

Protein Variants

Protein Variants Comment Organism
H170C site-directed mutagenesis, increased Km for prostaglandin PGD2 and 8fold decreased Km for prostaglandin PGH2 compared to the wild-type enzyme Bos taurus
K225S site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme Bos taurus
N306Y site-directed mutagenesis, increased Km for prostaglandin PGD2 and 5fold increased activity with prostaglandin PGH2 compared to the wild-type enzyme Bos taurus
R223L site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme Bos taurus
R27E site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme Bos taurus
R91Q site-directed mutagenesis, increased Km for prostaglandin PGD2 compared to the wild-type enzyme Bos taurus
S301R site-directed mutagenesis, slightly increased Km for prostaglandin PGD2 and PGH2 compared to the wild-type enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
9,10-phenanthrenequinone recombinant wild-type liver-type isozyme, 37°C Bos taurus
0.003
-
prostaglandin PGH2 recombinant liver-type isozyme, mutant H170C, 37°C Bos taurus
0.01
-
prostaglandin PGH2 recombinant liver-type isozyme, mutants R91Q, R223L and N306Y, 37°C Bos taurus
0.014
-
prostaglandin PGH2 recombinant liver-type isozyme, mutant R27E, 37°C Bos taurus
0.015
-
prostaglandin PGD2 recombinant wild-type liver-type isozyme, 37°C Bos taurus
0.025
-
prostaglandin PGH2 recombinant wild-type liver-type isozyme, 37°C Bos taurus
0.028
-
prostaglandin PGH2 recombinant liver-type isozyme, mutant K225S, 37°C Bos taurus
0.03
-
prostaglandin PGH2 recombinant liver-type isozyme, mutant S301R, 37°C Bos taurus
0.04
-
prostaglandin PGD2 recombinant liver-type isozyme mutant S301R, 37°C Bos taurus
0.05
-
prostaglandin PGD2 recombinant liver-type isozyme mutant K225S, 37°C Bos taurus
0.075
-
prostaglandin PGD2 recombinant liver-type isozyme mutant H170C, 37°C Bos taurus
0.1
-
prostaglandin PGD2 recombinant liver-type isozyme mutant N306Y, 37°C Bos taurus
0.11
-
prostaglandin PGD2 recombinant liver-type isozyme mutant R27E, 37°C Bos taurus
0.12
-
prostaglandin PGD2 recombinant wild-type lung-type isozyme Bos taurus
0.145
-
prostaglandin PGD2 recombinant liver-type isozyme mutant R91Q, 37°C Bos taurus
0.18
-
prostaglandin PGD2 recombinant liver-type isozyme mutant R223L, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm liver-type isozyme Bos taurus 5737
-
additional information no liver-type isozyme in mitochondrion, nucleus, or Golgi apparatus Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36666
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation Bos taurus
36670
-
lung-type isozyme, sequence calculation Bos taurus
36740
-
liver-type isozyme, sequence calculation Bos taurus
36742
-
1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
liver- and lung-type isozymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type liver-type isoyzme and mutant enzymes from COS-7 cells, to homogeneity Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
endothelium liver-type isozyme, sinusoidal endothelial cells in the liver Bos taurus
-
liver liver-type isozyme, sinusoidal endothelial cells Bos taurus
-
lung lung-type isozyme Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9,10-phenanthrenequinone + NADPH
-
Bos taurus ?
-
?
prostaglandin D2 + NADPH + H+ amino acid residues Arg27, Arg91, His170, Arg223, and Asn306 are involved in substrate binding Bos taurus prostaglandin 9alpha,11beta-F2 + NADP+
-
?
prostaglandin H2 + NADPH + H+ His170 is involved in binding, and Asn306 is critical for catalytic activity Bos taurus prostaglandin F2alpha + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36666, lung-type isozyme, sequence calculation, 1 * 36742, liver-type isozyme, sequence calculation Bos taurus

Synonyms

Synonyms Comment Organism
PGD2 11-ketoreductase
-
Bos taurus
PGFS
-
Bos taurus
PGH2 9,11-endoperoxidase
-
Bos taurus
prostaglandin F synthase
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Bos taurus