BRENDA - Enzyme Database show
show all sequences of 1.1.1.187

Guanosine diphosphate-4-keto-D-rhamnose reductase. A non-stereoselective enzyme

Winkler, N.W.; Markovitz, A.; J. Biol. Chem. 246, 5868-5876 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
GDP-alpha-D-mannose
competitive inhibitor
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
GDP-4-dehydro-6-deoxy-D-mannose
with NADPH as reductant
Pseudomonas sp.
0.027
-
NADH
-
Pseudomonas sp.
0.028
-
NADPH
-
Pseudomonas sp.
0.05
-
GDP-4-dehydro-6-deoxy-D-mannose
with NADH as reductant
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
70000
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
Pseudomonas sp.
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
GDP-6-deoxy-D-mannose + NAD(P)+
-
Pseudomonas sp.
?
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
Pseudomonas sp.
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
GDP-D-talometylose + NAD(P)+
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
soil bacterium GS, ATCC 19241
-
Oxidation Stability
Oxidation Stability
Organism
DTT reduces instability at room temperature from 51% to 35%
Pseudomonas sp.
Purification (Commentary)
Commentary
Organism
to homogeneity, fractionation-, chromatography-techniques
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.98
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-20°C, half-life: 5 months
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
389366
Pseudomonas sp.
GDP-6-deoxy-D-mannose + NAD(P)+
-
389366
Pseudomonas sp.
?
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
389366
Pseudomonas sp.
GDP-D-talometylose + NAD(P)+
-
389366
Pseudomonas sp.
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
56
-
loss of activity in 5 min
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
with NADPH as reductant
Pseudomonas sp.
8
-
with NADH as reductant
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
works equally well compared to NADPH
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.72
-
GDP-mannose
competitive inhibition
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
works equally well compared to NADPH
Pseudomonas sp.
NADPH
-
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
GDP-alpha-D-mannose
competitive inhibitor
Pseudomonas sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.72
-
GDP-mannose
competitive inhibition
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
GDP-4-dehydro-6-deoxy-D-mannose
with NADPH as reductant
Pseudomonas sp.
0.027
-
NADH
-
Pseudomonas sp.
0.028
-
NADPH
-
Pseudomonas sp.
0.05
-
GDP-4-dehydro-6-deoxy-D-mannose
with NADH as reductant
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
70000
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
Pseudomonas sp.
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
GDP-6-deoxy-D-mannose + NAD(P)+
-
Pseudomonas sp.
?
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
Pseudomonas sp.
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
GDP-D-talometylose + NAD(P)+
-
Pseudomonas sp.
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
DTT reduces instability at room temperature from 51% to 35%
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, fractionation-, chromatography-techniques
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.98
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, half-life: 5 months
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
389366
Pseudomonas sp.
GDP-6-deoxy-D-mannose + NAD(P)+
-
389366
Pseudomonas sp.
?
GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function
389366
Pseudomonas sp.
GDP-D-talometylose + NAD(P)+
-
389366
Pseudomonas sp.
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
56
-
loss of activity in 5 min
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
with NADPH as reductant
Pseudomonas sp.
8
-
with NADH as reductant
Pseudomonas sp.
Other publictions for EC 1.1.1.187
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
651142
Suzuki
Guanosine diphosphate-4-keto-6 ...
Aggregatibacter actinomycetemcomitans, Aggregatibacter actinomycetemcomitans SUNYaB 75
Eur. J. Biochem.
269
5963-5971
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389366
Winkler
Guanosine diphosphate-4-keto-D ...
Pseudomonas sp.
J. Biol. Chem.
246
5868-5876
1971
-
-
-
-
-
-
1
4
-
-
1
2
-
1
1
-
1
-
-
1
1
1
2
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
2
-
-
-
-
1
1
4
-
-
1
2
-
1
-
1
-
1
1
1
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
389367
Barber
The synthesis of guanosine 5-d ...
Leucaena leucocephala
Biochim. Biophys. Acta
165
68-75
1968
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-