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show all sequences of 1.1.1.185

Kinetics of alpha-dicarbonyls reduction by L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes

Carballo, J.; Bernardo, A.; Prieto, M.J.G.; Sarmiento, R.M.; Ital. J. Biochem. 42, 79-89 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetone
uncompetitive for NADH
Enterobacter aerogenes
L-acetoin
probably all L(+)-alpha-hydroxycarbonyls products inhibit uncompetitively at non-saturated concentrations
Enterobacter aerogenes
NAD+
competitive inhibition for NADH
Enterobacter aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADH
with ethyl pyruvate
Enterobacter aerogenes
0.0062
-
NADH
with 2,3-pentadione
Enterobacter aerogenes
0.0088
-
NADH
with diacetyl
Enterobacter aerogenes
0.0097
-
NADH
with methylglyoxal
Enterobacter aerogenes
1.5
-
diacetyl
-
Enterobacter aerogenes
10
-
2,3-Pentanedione
-
Enterobacter aerogenes
22
-
ethyl pyruvate
-
Enterobacter aerogenes
90
-
methylglyoxal
-
Enterobacter aerogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterobacter aerogenes
-
-
-
Reaction
Reaction
Commentary
Organism
an L-glycol + NAD(P)+ = a 2-hydroxycarbonyl compound + NAD(P)H + H+
ordered bi-bi-mechanism, coenzyme binds as first substrate to the enzyme
Enterobacter aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NADH
-
286137
Enterobacter aerogenes
L-acetylethylcarbinol + NAD+
-
286137
Enterobacter aerogenes
ir
diacetyl + NADH
-
286137
Enterobacter aerogenes
L-acetoin + NAD+
-
286137
Enterobacter aerogenes
ir
ethyl pyruvate + NAD(P)H
-
286137
Enterobacter aerogenes
ethyl lactate
-
286137
Enterobacter aerogenes
?
methylglyoxal + NADH
-
286137
Enterobacter aerogenes
L-lactaldehyde
-
286137
Enterobacter aerogenes
ir
R1-CO-CHOH-R2 + NAD(P)H
2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol
286137
Enterobacter aerogenes
R1-CHOH-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
r
R1-CO-CHOH-R2 + NAD(P)H
much more efficient in dehydrogenase direction
286137
Enterobacter aerogenes
R1-CHOH-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
r
R1-CO-CO-R2 + NAD(P)H
methylglyoxal, diacetyl, 2,3-pentanedione
286137
Enterobacter aerogenes
R1-CO-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
ir
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Enterobacter aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Enterobacter aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
NADH
-
Enterobacter aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Enterobacter aerogenes
NADH
-
Enterobacter aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetone
uncompetitive for NADH
Enterobacter aerogenes
L-acetoin
probably all L(+)-alpha-hydroxycarbonyls products inhibit uncompetitively at non-saturated concentrations
Enterobacter aerogenes
NAD+
competitive inhibition for NADH
Enterobacter aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADH
with ethyl pyruvate
Enterobacter aerogenes
0.0062
-
NADH
with 2,3-pentadione
Enterobacter aerogenes
0.0088
-
NADH
with diacetyl
Enterobacter aerogenes
0.0097
-
NADH
with methylglyoxal
Enterobacter aerogenes
1.5
-
diacetyl
-
Enterobacter aerogenes
10
-
2,3-Pentanedione
-
Enterobacter aerogenes
22
-
ethyl pyruvate
-
Enterobacter aerogenes
90
-
methylglyoxal
-
Enterobacter aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NADH
-
286137
Enterobacter aerogenes
L-acetylethylcarbinol + NAD+
-
286137
Enterobacter aerogenes
ir
diacetyl + NADH
-
286137
Enterobacter aerogenes
L-acetoin + NAD+
-
286137
Enterobacter aerogenes
ir
ethyl pyruvate + NAD(P)H
-
286137
Enterobacter aerogenes
ethyl lactate
-
286137
Enterobacter aerogenes
?
methylglyoxal + NADH
-
286137
Enterobacter aerogenes
L-lactaldehyde
-
286137
Enterobacter aerogenes
ir
R1-CO-CHOH-R2 + NAD(P)H
2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol
286137
Enterobacter aerogenes
R1-CHOH-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
r
R1-CO-CHOH-R2 + NAD(P)H
much more efficient in dehydrogenase direction
286137
Enterobacter aerogenes
R1-CHOH-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
r
R1-CO-CO-R2 + NAD(P)H
methylglyoxal, diacetyl, 2,3-pentanedione
286137
Enterobacter aerogenes
R1-CO-CHOH-R2 + NAD(P)+
-
286137
Enterobacter aerogenes
ir
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Enterobacter aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Enterobacter aerogenes
Other publictions for EC 1.1.1.185
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286137
Carballo
Kinetics of alpha-dicarbonyls ...
Enterobacter aerogenes
Ital. J. Biochem.
42
79-89
1993
-
-
-
-
-
-
3
8
-
-
-
-
-
2
-
-
-
1
-
-
-
-
7
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286136
Prieto
Kinetics of alpha-dicarbonyls ...
Gallus gallus
Arch. Biochem. Biophys.
224
372-377
1983
-
-
-
-
-
-
1
8
-
-
-
-
-
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
8
-
-
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286135
Burgos
L-Glycol dehydrogenase from he ...
Gallus gallus
Methods Enzymol.
89
523-526
1982
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
1
-
1
1
1
1
-
1
-
-
-
1
-
1
3
-
-
-
-
-
-
3
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
286134
Bernardo
Purification and some properti ...
Gallus gallus
Biochim. Biophys. Acta
659
189-198
1981
-
-
-
-
-
2
-
17
-
-
1
-
-
1
-
-
1
-
-
1
1
1
10
-
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
2
-
-
-
17
-
-
1
-
-
-
-
1
-
1
1
1
10
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-