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show all sequences of 1.1.1.185

Purification and some properties of L-glycol dehydrogenase from hens muscle

Bernardo, A.; Burgos, J.; Martin, R.; Biochim. Biophys. Acta 659, 189-198 (1981)

Data extracted from this reference:

General Stability
General Stability
Organism
easily inactivated in high molarity buffers
Gallus gallus
very stable in both water and low molarity buffers of pH 7 at 0-4C
Gallus gallus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Gallus gallus
0.026
-
glyceraldehyde
pI 7.2 form
Gallus gallus
0.053
-
Glyoxal
pI 6.2 form
Gallus gallus
0.06
-
2,3-Pentanedione
pI 7.2 form
Gallus gallus
0.097
-
2,3-Pentanedione
pI 6.2 form
Gallus gallus
0.098
-
methylglyoxal
pI 6.2 form
Gallus gallus
0.102
-
methylglyoxal
pI 7.2 form
Gallus gallus
0.109
-
diacetyl
pI 7.2 form
Gallus gallus
0.29
-
glyceraldehyde
pI 6.2 form
Gallus gallus
0.38
-
diacetyl
pI 6.2 form
Gallus gallus
0.411
-
Glyoxal
pI 4.8 form
Gallus gallus
0.522
-
Glyoxal
pI 7.2 form
Gallus gallus
0.566
-
glyceraldehyde
pI 4.8 form
Gallus gallus
0.675
-
2,3-Pentanedione
pI 4.8 form
Gallus gallus
2.013
-
acetoin
pI 6.2 form
Gallus gallus
2.164
-
acetoin
pI 4.8 form
Gallus gallus
2.59
-
acetoin
pI 7.2 form
Gallus gallus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel filtration
Gallus gallus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gallus gallus
-
hen
-
Purification (Commentary)
Commentary
Organism
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8
Gallus gallus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
muscle
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Gallus gallus
Storage Stability
Storage Stability
Organism
-18C, enzyme at any stage of purification
Gallus gallus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NADH
-
286134
Gallus gallus
L-acetylethylcarbinol + NAD+
-
-
-
-
diacetyl + NADH
-
286134
Gallus gallus
L-acetoin + NAD+
-
-
-
-
ethyl pyruvate + NAD(P)H
-
286134
Gallus gallus
ethyl lactate
-
-
-
?
methyl pyruvate + NAD(P)H
-
286134
Gallus gallus
?
-
-
-
?
methylglyoxal + NADH
-
286134
Gallus gallus
L-lactaldehyde
-
-
-
-
additional information
not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids
286134
Gallus gallus
?
-
-
-
-
R1-CO-CHOH-R2 + NAD(P)H
2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol
286134
Gallus gallus
R1-CHOH-CHOH-R2 + NAD(P)+
-
286134
Gallus gallus
r
R1-CO-CHOH-R2 + NAD(P)H
much more efficient in dehydrogenase direction
286134
Gallus gallus
R1-CHOH-CHOH-R2 + NAD(P)+
-
286134
Gallus gallus
r
R1-CO-CO-R2 + NAD(P)H
methylglyoxal, diacetyl, 2,3-pentanedione
286134
Gallus gallus
R1-CO-CHOH-R2 + NAD(P)+
-
-
-
?
R1-CO-CO-R2 + NAD(P)H
vicinal dicarbonyl: e.g. glyoxal
286134
Gallus gallus
R1-CO-CHOH-R2 + NAD(P)+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Gallus gallus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6.6
-
Gallus gallus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
very stable in both water and low molarity buffers of pH 7 at 0-4C
Gallus gallus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with alpha-NADH
Gallus gallus
NAD+
-
Gallus gallus
NADH
-
Gallus gallus
NADPH
-
Gallus gallus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with alpha-NADH
Gallus gallus
NAD+
-
Gallus gallus
NADH
-
Gallus gallus
NADPH
-
Gallus gallus
General Stability (protein specific)
General Stability
Organism
easily inactivated in high molarity buffers
Gallus gallus
very stable in both water and low molarity buffers of pH 7 at 0-4C
Gallus gallus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Gallus gallus
0.026
-
glyceraldehyde
pI 7.2 form
Gallus gallus
0.053
-
Glyoxal
pI 6.2 form
Gallus gallus
0.06
-
2,3-Pentanedione
pI 7.2 form
Gallus gallus
0.097
-
2,3-Pentanedione
pI 6.2 form
Gallus gallus
0.098
-
methylglyoxal
pI 6.2 form
Gallus gallus
0.102
-
methylglyoxal
pI 7.2 form
Gallus gallus
0.109
-
diacetyl
pI 7.2 form
Gallus gallus
0.29
-
glyceraldehyde
pI 6.2 form
Gallus gallus
0.38
-
diacetyl
pI 6.2 form
Gallus gallus
0.411
-
Glyoxal
pI 4.8 form
Gallus gallus
0.522
-
Glyoxal
pI 7.2 form
Gallus gallus
0.566
-
glyceraldehyde
pI 4.8 form
Gallus gallus
0.675
-
2,3-Pentanedione
pI 4.8 form
Gallus gallus
2.013
-
acetoin
pI 6.2 form
Gallus gallus
2.164
-
acetoin
pI 4.8 form
Gallus gallus
2.59
-
acetoin
pI 7.2 form
Gallus gallus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
gel filtration
Gallus gallus
Purification (Commentary) (protein specific)
Commentary
Organism
3 enzyme forms: pI 7.2, pI 6.2 and pI 4.8
Gallus gallus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
muscle
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Gallus gallus
Storage Stability (protein specific)
Storage Stability
Organism
-18C, enzyme at any stage of purification
Gallus gallus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NADH
-
286134
Gallus gallus
L-acetylethylcarbinol + NAD+
-
-
-
-
diacetyl + NADH
-
286134
Gallus gallus
L-acetoin + NAD+
-
-
-
-
ethyl pyruvate + NAD(P)H
-
286134
Gallus gallus
ethyl lactate
-
-
-
?
methyl pyruvate + NAD(P)H
-
286134
Gallus gallus
?
-
-
-
?
methylglyoxal + NADH
-
286134
Gallus gallus
L-lactaldehyde
-
-
-
-
additional information
not: acetaldehyde, monoketones, non-vicinal diketones, free ketoacids
286134
Gallus gallus
?
-
-
-
-
R1-CO-CHOH-R2 + NAD(P)H
2-hydroxycarbonyl compound e.g. glycolaldehyde, glyceraldehyde, acetoin, acetylethylcarbinol, diacetylmethylcarbinol
286134
Gallus gallus
R1-CHOH-CHOH-R2 + NAD(P)+
-
286134
Gallus gallus
r
R1-CO-CHOH-R2 + NAD(P)H
much more efficient in dehydrogenase direction
286134
Gallus gallus
R1-CHOH-CHOH-R2 + NAD(P)+
-
286134
Gallus gallus
r
R1-CO-CO-R2 + NAD(P)H
methylglyoxal, diacetyl, 2,3-pentanedione
286134
Gallus gallus
R1-CO-CHOH-R2 + NAD(P)+
-
-
-
?
R1-CO-CO-R2 + NAD(P)H
vicinal dicarbonyl: e.g. glyoxal
286134
Gallus gallus
R1-CO-CHOH-R2 + NAD(P)+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Gallus gallus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6.6
-
Gallus gallus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
very stable in both water and low molarity buffers of pH 7 at 0-4C
Gallus gallus
Other publictions for EC 1.1.1.185
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286137
Carballo
Kinetics of alpha-dicarbonyls ...
Enterobacter aerogenes
Ital. J. Biochem.
42
79-89
1993
-
-
-
-
-
-
3
8
-
-
-
-
-
2
-
-
-
1
-
-
-
-
7
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
8
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286136
Prieto
Kinetics of alpha-dicarbonyls ...
Gallus gallus
Arch. Biochem. Biophys.
224
372-377
1983
-
-
-
-
-
-
1
8
-
-
-
-
-
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
8
-
-
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286135
Burgos
L-Glycol dehydrogenase from he ...
Gallus gallus
Methods Enzymol.
89
523-526
1982
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
1
-
1
1
1
1
-
1
-
-
-
1
-
1
3
-
-
-
-
-
-
3
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
286134
Bernardo
Purification and some properti ...
Gallus gallus
Biochim. Biophys. Acta
659
189-198
1981
-
-
-
-
-
2
-
17
-
-
1
-
-
1
-
-
1
-
-
1
1
1
10
-
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
2
-
-
-
17
-
-
1
-
-
-
-
1
-
1
1
1
10
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-