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Literature summary for 1.1.1.184 extracted from

  • Bleicher, K.; Winter, J.
    Purification and properties of F420- and NADP(+)-dependent alcohol dehydrogenases of Methanogenium liminatans and Methanobacterium palustre, specific for secondary alcohols (1991), Eur. J. Biochem., 200, 43-51.
    View publication on PubMed
Search for more literature for 1.1.1.184

General Stability

General Stability Organism
an ammonium sulfate precipitate (3 M, pH 7) of the enzyme loses its activity completely within a few days Methanobacterium palustre
minor loss of activity in 50 mM phosphate buffer is observed during purification and storage at 4°C without additional precautions Methanobacterium palustre
no conditions to stabilize the enzyme by freezing at -20°C are found. Addition of 1-10% bovine serum albumin, 20-50% glycerol, 20% 1,2-ethanediol or 0.1% Triton X-100 to the enzyme solution in 40 mM phosphate or 40 mM Tris-HC1 pH 7 before freezing for 1 day or longer has no stabilizing effect Methanobacterium palustre

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 5 mM, 17% inhibition Methanobacterium palustre
4-hydroxymercuribenzoate 0.025 mM, complete inhibition Methanobacterium palustre
4-Methylpyrazole 2 mM, 17% inhibition Methanobacterium palustre
iodoacetate 2 mM, complete inhibition Methanobacterium palustre
additional information no inhibition by 5 mM 2,2'-dipyridyl, EDTA does not inhibit up to 5 mM Methanobacterium palustre

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADP+ pH 7.5, 37°C Methanobacterium palustre
0.025
-
 NADPH pH 7.5, 37°C Methanobacterium palustre
0.4
-
 propanone pH 7.5, 37°C Methanobacterium palustre
2.2
-
 (R)-2-butanol pH 7.5, 37°C Methanobacterium palustre
3
-
 2-propanol pH 7.5, 37°C Methanobacterium palustre
3
-
 diacetyl pH 7.5, 37°C Methanobacterium palustre
3.5
-
 2-butanol pH 7.5, 37°C Methanobacterium palustre
4.2
-
 Cyclopentanone pH 7.5, 37°C Methanobacterium palustre
4.5
-
 (S)-2-butanol pH 7.5, 37°C Methanobacterium palustre
4.5
-
 Cyclopentanol pH 7.5, 37°C Methanobacterium palustre
6.5
-
 cyclohexanone pH 7.5, 37°C Methanobacterium palustre
9.5
-
 2-butanone pH 7.5, 37°C Methanobacterium palustre
14
-
 2-Pentanone pH 7.5, 37°C Methanobacterium palustre
16
-
 acetoin pH 7.5, 37°C Methanobacterium palustre
18
-
 2,3-Butanediol pH 7.5, 37°C Methanobacterium palustre

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal activity is observed in the presence of 500 mM NaCl or KCl Methanobacterium palustre
NaCl optimal activity is observed in the presence of 500 mM NaCl or KCl Methanobacterium palustre
Zn2+ the enzyme contains 4-8 mol atoms zinc/mol enzyme Methanobacterium palustre

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 4 * 44000, SDS-PAGE Methanobacterium palustre
175000
-
 gel filtration Methanobacterium palustre

Organism

Organism UniProt Comment Textmining
Methanobacterium palustre
-
-
-
Methanobacterium palustre DSM 3108
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanobacterium palustre

Storage Stability

Storage Stability Organism
4°C, pH 7, protein concentration above 1.5 mg/ml, strictly anaerobic conditions in 50 mM phosphate, stable for up to 1 year without significant loss of activity Methanobacterium palustre

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-butanol + NADP+
-
 Methanobacterium palustre 2-butanone + NADPH + H+
-
 ?
(R)-2-butanol + NADP+
-
 Methanobacterium palustre DSM 3108 2-butanone + NADPH + H+
-
 ?
(S)-2-butanol + NADP+
-
 Methanobacterium palustre 2-butanone + NADPH + H+
-
 ?
(S)-2-butanol + NADP+
-
 Methanobacterium palustre DSM 3108 2-butanone + NADPH + H+
-
 ?
2,3-butanediol + NADP+
-
 Methanobacterium palustre acetoin + NADPH + H+
-
 ?
2-butanol + NADP+
-
 Methanobacterium palustre 2-butanone + NADPH + H+
-
 r
2-pentanol + NADP+
-
 Methanobacterium palustre 2-pentanone + NADPH + H+
-
 r
2-propanol + NADP+
-
 Methanobacterium palustre propanone + NADPH + H+ 2-propanone i.e. acetone r
cycloheptanone + NADPH + H+
-
 Methanobacterium palustre cycloheptanol + NADP+
-
 ?
cyclohexanol + NADP+
-
 Methanobacterium palustre cyclohexanone + NADPH + H+
-
 r
cyclohexanol + NADP+
-
 Methanobacterium palustre DSM 3108 cyclohexanone + NADPH + H+
-
 r
cyclopentanol + NADP+
-
 Methanobacterium palustre cyclopentanone + NADPH + H+
-
 r
diacetyl + NADPH + H+
-
 Methanobacterium palustre acetoin + NADP+
-
 ?
diacetyl + NADPH + H+
-
 Methanobacterium palustre DSM 3108 acetoin + NADP+
-
 ?
additional information the enzyme catalyses the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-Butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1-propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced Methanobacterium palustre ?
-
 ?
additional information the enzyme catalyses the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-Butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1-propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced Methanobacterium palustre DSM 3108 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Methanobacterium palustre

Synonyms

Synonyms Comment Organism
NADP+-dependent ADH
-
 Methanobacterium palustre

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanobacterium palustre
60
-
-
 Methanobacterium palustre

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 Cyclohexanol pH 7.5, 37°C Methanobacterium palustre
5
-
 2-Pentanol pH 7.5, 37°C Methanobacterium palustre
6
-
 Cycloheptanone pH 7.5, 37°C Methanobacterium palustre
18
-
 2-Pentanone pH 7.5, 37°C Methanobacterium palustre
26
-
 2,3-Butanediol pH 7.5, 37°C Methanobacterium palustre
80
-
 Cyclopentanone pH 7.5, 37°C Methanobacterium palustre
86
-
 2-butanone pH 7.5, 37°C Methanobacterium palustre
86
-
 cyclohexanone pH 7.5, 37°C Methanobacterium palustre
106
-
 Cyclopentanol pH 7.5, 37°C Methanobacterium palustre
123
-
 (S)-2-butanol pH 7.5, 37°C Methanobacterium palustre
129
-
 NADPH pH 7.5, 37°C Methanobacterium palustre
129
-
 propanone pH 7.5, 37°C Methanobacterium palustre
149
-
 2-butanol pH 7.5, 37°C Methanobacterium palustre
177
-
 (R)-2-butanol pH 7.5, 37°C Methanobacterium palustre
183
-
 acetoin pH 7.5, 37°C Methanobacterium palustre
243
-
 NADP+ pH 7.5, 37°C Methanobacterium palustre
243
-
 2-propanol pH 7.5, 37°C Methanobacterium palustre
609
-
 diacetyl pH 7.5, 37°C Methanobacterium palustre

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methanobacterium palustre

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 2-Pentanone pH 7.5, 37°C Methanobacterium palustre
1.4
-
 2,3-Butanediol pH 7.5, 37°C Methanobacterium palustre
9
-
 2-butanone pH 7.5, 37°C Methanobacterium palustre
11
-
 acetoin pH 7.5, 37°C Methanobacterium palustre
13
-
 cyclohexanone pH 7.5, 37°C Methanobacterium palustre
19
-
 Cyclopentanone pH 7.5, 37°C Methanobacterium palustre
23
-
 Cyclopentanol pH 7.5, 37°C Methanobacterium palustre
27
-
 (S)-2-butanol pH 7.5, 37°C Methanobacterium palustre
42
-
 2-butanol pH 7.5, 37°C Methanobacterium palustre
80
-
 (R)-2-butanol pH 7.5, 37°C Methanobacterium palustre
81
-
 2-propanol pH 7.5, 37°C Methanobacterium palustre
203
-
 diacetyl pH 7.5, 37°C Methanobacterium palustre
321
-
 propanone pH 7.5, 37°C Methanobacterium palustre
5200
-
 NADPH pH 7.5, 37°C Methanobacterium palustre
11600
-
 NADP+ pH 7.5, 37°C Methanobacterium palustre