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Literature summary for 1.1.1.184 extracted from

  • Tang, X.; Mu, P.; Wu, J.; Jiang, J.; Zhang, C.; Zheng, M.; Deng, Y.
    Carbonyl reduction of mequindox by chicken and porcine cytosol and cloned carbonyl reductase 1 (2012), Drug Metab. Dispos., 40, 788-795.
    View publication on PubMed
Search for more literature for 1.1.1.184

Application

Application Comment Organism
food industry mequindox, inhibitor of several Gram-positive and Gram-negative bacteria, is reduced by carbonyl reductase CBR1 Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sus scrofa
expression in Escherichia coli Gallus gallus

Protein Variants

Protein Variants Comment Organism
E142M increase in reaction velocity Sus scrofa
E142V increase in reaction velocity Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0199
-
 menadione pH 7.4, 37°C Sus scrofa
0.076
-
 menadione pH 7.4, 37°C Gallus gallus
0.105
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
0.116
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa
0.156
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
0.469
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-
cytosol
-
 Gallus gallus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Sus scrofa
31000
-
 x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Gallus gallus
31590
-
 x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Sus scrofa
31590
-
 x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus F1N8Y3 putative
-
Sus scrofa Q28960
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-
liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone + NADPH + H+ i.e. mequindox, synthetic quinoxaline derivative, inhibitory to several Gram-positive and Gram-negative bacteria Sus scrofa 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanol + NADP+
-
 ?
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone + NADPH + H+ i.e. mequindox, synthetic quinoxaline derivative, inhibitory to several Gram-positive and Gram-negative bactria Gallus gallus 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanol + NADP+
-
 ?
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone + NADPH + H+
-
 Sus scrofa 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanol + NADP+
-
 ?
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone + NADPH + H+
-
 Gallus gallus 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanol + NADP+
-
 ?
menadione + NADPH + H+
-
 Sus scrofa ? + NADP+
-
 ?
menadione + NADPH + H+
-
 Gallus gallus ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Sus scrofa
? x * 31000, SDS-PAGE, x * 31590, calculated, His-tagged protein Gallus gallus

Synonyms

Synonyms Comment Organism
CBR1
-
 Sus scrofa
CBR1
-
 Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00027
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa
0.00062
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
0.15
-
 menadione pH 7.4, 37°C Sus scrofa
2.72
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
2.89
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa
4.17
-
 menadione pH 7.4, 37°C Gallus gallus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
5.75
-
 1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa
7.5
-
 menadione pH 7.4, 37°C Sus scrofa
25
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Sus scrofa
26
-
 1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone pH 7.4, 37°C Gallus gallus
54.5
-
 menadione pH 7.4, 37°C Gallus gallus