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Literature summary for 1.1.1.184 extracted from

  • Schweiger, P.; Gross, H.; Deppenmeier, U.
    Characterization of two aldo-keto reductases from Gluconobacter oxydans 621H capable of regio- and stereoselective alpha-ketocarbonyl reduction (2010), Appl. Microbiol. Biotechnol., 87, 1415-1426.
    View publication on PubMed
Search for more literature for 1.1.1.184

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: up to 1.0 mM Zn2+, Mg2+, Mn2+, Fe2+, or EDTA Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0612
-
 NADPH pH 7.0, 25°C Gluconobacter oxydans
0.075
-
 NADPH pH 7.0, 25°C Gluconobacter oxydans
2.2
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans
4.8
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans
5.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
10.8
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31600
-
 2 * 31600, SDS-PAGE Gluconobacter oxydans
66100
-
 PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q5FQJ0
-
-
Gluconobacter oxydans Q5FT75
-
-
Gluconobacter oxydans 621H Q5FQJ0
-
-
Gluconobacter oxydans 621H Q5FT75
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 substrate ethyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
3.4
-
 substrate methylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
4.7
-
 substrate isoamyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
5.4
-
 substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C Gluconobacter oxydans
6
-
 substrate 2,3-butanedione, pH 7.0, 25°C Gluconobacter oxydans
6.5
-
 substrate 2,3-hexanedione, pH 7.0, 25°C Gluconobacter oxydans
17
-
 substrate 2,3-pentanedione, pH 7.0, 25°C Gluconobacter oxydans
19.1
-
 substrate phenylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
35
-
 substrate methylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
50.1
-
 substrate 2,3-hexanedione, pH 7.0, 25°C Gluconobacter oxydans
60
-
 substrate 2,3-butanedione, pH 7.0, 25°C Gluconobacter oxydans
63.1
-
 substrate isoamyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
65
-
 substrate 2,5-diketo-D-gluconate, pH 7.0, 25°C Gluconobacter oxydans
69.6
-
 substrate 2,3-pentanedione, pH 7.0, 25°C Gluconobacter oxydans
74.7
-
 substrate phenylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
75.4
-
 substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C Gluconobacter oxydans
92.8
-
 substrate ethyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans (R)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans 621H (R)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans (S)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans 621H (S)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
2,3-butanedione + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
2,3-butanedione + NADPH + H+
-
 Gluconobacter oxydans 621H ? + NADP+
-
 ?
2,3-hexanedione + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
2,3-hexanedione + NADPH + H+
-
 Gluconobacter oxydans 621H ? + NADP+
-
 ?
2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans (R)-2-hydroxy-pentane-3-one + NADP+
-
 ?
2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans 621H (R)-2-hydroxy-pentane-3-one + NADP+
-
 ?
2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans (S)-2-hydroxy-pentane-3-one + NADP+
-
 ?
2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans 621H (S)-2-hydroxy-pentane-3-one + NADP+
-
 ?
2,5-diketo-D-gluconate + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
ethyl pyruvate + NADPH + H+
-
 Gluconobacter oxydans ethyl lactate + NADP+
-
 ?
isoamyl pyruvate + NADPH + H+
-
 Gluconobacter oxydans isoamyl lactate + NADP+
-
 ?
methylglyoxal + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone Gluconobacter oxydans ?
-
 ?
additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone Gluconobacter oxydans ?
-
 ?
additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone Gluconobacter oxydans 621H ?
-
 ?
additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone Gluconobacter oxydans 621H ?
-
 ?
phenylglyoxal + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31600, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
Gox0644
-
 Gluconobacter oxydans
Gox1615
-
 Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
23.8
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans
36.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
39.3
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Gluconobacter oxydans
7
-
-
 Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.99
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
6.4
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
8.2
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans
11
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans