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Literature summary for 1.1.1.184 extracted from

  • Saratani, Y.; Uheda, E.; Yamamoto, H.; Nishimura, A.; Yoshizako, F.
    Purification and properties of a carbonyl reductase involved in stereoselective reduction of ethyl 4-chloro-3-oxobutanoate from Cylindrocarpon sclerotigenum IFO 31855 (2003), Biosci. Biotechnol. Biochem., 67, 1417-1420.
    View publication on PubMed

Application

Application Comment Organism
synthesis enzyme might be useful for production of ethyl (S)-4-chloro-3-hydroxybutanoate, which is used in the synthesis of pharmacologically and biologically important compounds Cylindrocarpon sclerotigenum

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenol 19% inhibition at 0.1 mM Cylindrocarpon sclerotigenum
Cd2+ 16% inhibition at 1 mM Cylindrocarpon sclerotigenum
Cu2+ 46% inhibition at 1 mM Cylindrocarpon sclerotigenum
dicoumarol 42% inhibition at 0.3 mM Cylindrocarpon sclerotigenum
DTNB 56% inhibition at 0.05 mM Cylindrocarpon sclerotigenum
EDTA 30% inhibition at 1 mM Cylindrocarpon sclerotigenum
Hg2+ complete inhibition at 1 mM Cylindrocarpon sclerotigenum
additional information no inhibition by diphenylhydantoin, N-ethylmaleimide, p-chloromercuribenzoate, PMSF, Mg2+, and Zn2+ Cylindrocarpon sclerotigenum
quercetin 16% inhibition at 0.1 mM Cylindrocarpon sclerotigenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24800
-
x * 24800, SDS-PAGE Cylindrocarpon sclerotigenum
68000
-
gel filtration Cylindrocarpon sclerotigenum

Organism

Organism UniProt Comment Textmining
Cylindrocarpon sclerotigenum
-
enzyme CSCR1, strain IFO 31855
-

Purification (Commentary)

Purification (Comment) Organism
by sequential column chromatographies involving DEAE ion exchange, phenyl resin, and hydroxylapatite chromatography Cylindrocarpon sclerotigenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
125.3
-
purified enzyme Cylindrocarpon sclerotigenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanedione + NADPH
-
Cylindrocarpon sclerotigenum ? + NADP+
-
ir
2,3-pentanedione + NADPH + H+ low activity Cylindrocarpon sclerotigenum ? + NADP+
-
ir
D-xylose + NADPH very low activity Cylindrocarpon sclerotigenum ? + NADP+
-
ir
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ highly stereoselective reduction Cylindrocarpon sclerotigenum (S)-ethyl 4-chloro-3-hydroxybutanoate + NADP+
-
ir
ethylpyruvate + NADPH low activity Cylindrocarpon sclerotigenum ? + NADP+
-
ir
hydroxyacetone + NADPH low activity Cylindrocarpon sclerotigenum ? + NADP+
-
ir
additional information enzyme shows highly restricted substrate specificity, overview Cylindrocarpon sclerotigenum ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 24800, SDS-PAGE Cylindrocarpon sclerotigenum

Synonyms

Synonyms Comment Organism
carbonyl reductase
-
Cylindrocarpon sclerotigenum
CSCR1
-
Cylindrocarpon sclerotigenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Cylindrocarpon sclerotigenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Cylindrocarpon sclerotigenum

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Cylindrocarpon sclerotigenum
NADPH dependent on, no reverse reaction with NADP+ as cofactor Cylindrocarpon sclerotigenum