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Literature summary for 1.1.1.184 extracted from
- Expression, purification and preliminary x-ray diffraction analysis of a ketoreductase from a type II polyketide synthase (2004), Acta Crystallogr. Sect. D, D60, 1137-1138.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional overexpression as His-tagged protein in Escherichia coli BL21(DE3) | Streptomyces coelicolor |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 5 mg/ml recombinant enzyme in 25 mM phosphate buffer, pH 8.0, with 10% glycerol, and 5 mM NADP+, hanging drop vapour diffusion method, against well solution containing 4.0-4.4 M sodium formate and 10% v/v glycerol, X-ray diffraction structure determination and analysis at 2.5 A resolution | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
actIII orf5 gene, ketoreductase type II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged active enzyme from Escherichia coli | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyketid + NADPH | enzyme acts on the nascent polyketid chain from polyketid synthase reaction and reduces the carbonyl group at C-9 | Streptomyces coelicolor | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | in presence of NADPH, in absence of cofactor the enzyme forms mixtures of dimer, monomer, and tetramer, SDS-PAGE and native PAGE | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ketoreductase | - |
Streptomyces coelicolor |
| KR | - |
Streptomyces coelicolor |
| More | enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Streptomyces coelicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Streptomyces coelicolor | |
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