BRENDA - Enzyme Database show
show all sequences of 1.1.1.183

Monoterpene alcohol metabolism: identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from Polygonum minus leaves

Hassan, M.; Maarof, N.D.; Ali, Z.M.; Noor, N.M.; Othman, R.; Mori, N.; Biosci. Biotechnol. Biochem. 76, 1463-1470 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity
Persicaria minor
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM
Persicaria minor
cyanide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
iodoacetamide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
MgCl2
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM
Persicaria minor
Sodium azide
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM
Persicaria minor
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
NADP+
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.0588
-
NADP+
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.185
-
geraniol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.4
-
geraniol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.435
-
cinnamyl alcohol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.556
-
cinnamyl alcohol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.714
-
nerol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.741
-
linalool
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
1.212
-
nerol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
1.333
-
linalool
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
2.222
-
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
10
-
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
58000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
60000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
62000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
100000
-
isoform geraniol-DH I, gel filtration
Persicaria minor
120000
-
isoform geraniol-DH II, gel filtration
Persicaria minor
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Persicaria minor
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography
Persicaria minor
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Persicaria minor
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.002
-
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.175
-
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.261
-
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-citronellol + NADP+
compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
borneol + NADP+
compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
carveol + NADP+
compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
cinnamyl alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
geraniol + NADP+
100% activity
719124
Persicaria minor
geranial + NADPH + H+
-
-
-
?
linalool + NADP+
compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
additional information
tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol
719124
Persicaria minor
?
-
-
-
-
nerol + NADP+
compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
p-cumic alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
isoforms geraniol-DH I and geraniol-DH II
Persicaria minor
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
70
rapid inactiviation of isoform geraniol-DH I occurs from 30-60C with 60% activity at 40C and 40% residual activity at 60C. At 70C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40C, while from 30-50C the activity decreases slightly and is lost at temperatures above 50C
Persicaria minor
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
isoform geraniol-DH I
Persicaria minor
9.5
-
isoform geraniol-DH II
Persicaria minor
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below
Persicaria minor
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Persicaria minor
isoform geraniol-DH II, isoelectric focusing
-
3.5
Persicaria minor
isoform geraniol-DH I, isoelectric focusing
-
8.8
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity
Persicaria minor
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM
Persicaria minor
cyanide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
iodoacetamide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
MgCl2
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM
Persicaria minor
Sodium azide
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM
Persicaria minor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
NADP+
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.0588
-
NADP+
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.185
-
geraniol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.4
-
geraniol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.435
-
cinnamyl alcohol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.556
-
cinnamyl alcohol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.714
-
nerol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.741
-
linalool
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
1.212
-
nerol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
1.333
-
linalool
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
2.222
-
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
10
-
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
58000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
60000
-
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
62000
-
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
100000
-
isoform geraniol-DH I, gel filtration
Persicaria minor
120000
-
isoform geraniol-DH II, gel filtration
Persicaria minor
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography
Persicaria minor
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Persicaria minor
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.002
-
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.175
-
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
0.261
-
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37C
Persicaria minor
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-citronellol + NADP+
compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
borneol + NADP+
compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
carveol + NADP+
compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
cinnamyl alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
geraniol + NADP+
100% activity
719124
Persicaria minor
geranial + NADPH + H+
-
-
-
?
linalool + NADP+
compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
additional information
tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol
719124
Persicaria minor
?
-
-
-
-
nerol + NADP+
compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
p-cumic alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate
719124
Persicaria minor
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
isoforms geraniol-DH I and geraniol-DH II
Persicaria minor
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
70
rapid inactiviation of isoform geraniol-DH I occurs from 30-60C with 60% activity at 40C and 40% residual activity at 60C. At 70C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40C, while from 30-50C the activity decreases slightly and is lost at temperatures above 50C
Persicaria minor
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
isoform geraniol-DH I
Persicaria minor
9.5
-
isoform geraniol-DH II
Persicaria minor
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below
Persicaria minor
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Persicaria minor
isoform geraniol-DH II, isoelectric focusing
-
3.5
Persicaria minor
isoform geraniol-DH I, isoelectric focusing
-
8.8
Other publictions for EC 1.1.1.183
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719124
Hassan
Monoterpene alcohol metabolism ...
Persicaria minor
Biosci. Biotechnol. Biochem.
76
1463-1470
2012
1
-
-
-
-
-
5
12
-
-
6
-
-
3
-
-
1
-
-
1
3
-
9
1
1
-
1
-
2
1
-
-
-
2
-
1
-
-
-
-
-
-
-
5
-
12
-
-
6
-
-
-
-
1
-
1
3
-
9
1
1
-
1
-
2
1
-
2
-
-
-
-
-
-
723856
Saito
Crystallization and preliminar ...
Backhousia citriodora
Acta Crystallogr. Sect. F
67
665-667
2011
-
-
1
1
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724585
Yamaguchi
The effects of high hydrostati ...
Zingiber officinale
Biosci. Biotechnol. Biochem.
74
1981-1986
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
1
-
-
-
-
2
-
-
1
1
-
3
-
-
1
-
-
-
1
-
4
1
1
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
667325
Iijima
Analysis of the enzymatic form ...
Ocimum basilicum
Arch. Biochem. Biophys.
448
141-149
2006
-
-
1
-
-
-
1
2
-
1
-
2
-
2
-
-
1
-
-
1
-
-
14
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
1
-
2
-
-
-
1
-
1
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
670514
Luan
Metabolism of geraniol in grap ...
Vitis vinifera
Phytochemistry
66
295-303
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286132
Sekiwa-Iijima
Geraniol dehydrogenase activit ...
Zingiber officinale
J. Agric. Food Chem.
49
5902-5906
2001
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
1
-
2
1
-
12
-
1
-
-
-
2
2
2
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
1
-
12
-
1
-
-
-
2
2
2
-
-
-
-
-
-
-
286131
Sangwan
-
Metabolism of acyclic monoterp ...
Cymbopogon flexuosus
J. Plant Physiol.
142
129-134
1993
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
1
-
-
1
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-
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11
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