BRENDA - Reference to 1.1.1.183; Id = 719124

additional information

adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity

Persicaria minor

2,2'-dipyridyl

isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM

Persicaria minor

cyanide

isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM

Persicaria minor

iodoacetamide

isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM

Persicaria minor

MgCl2

isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM

Persicaria minor

Sodium azide

isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM

Persicaria minor

0.021

-

NADP+

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.0588

-

NADP+

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.185

-

geraniol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.4

-

geraniol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.435

-

cinnamyl alcohol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.556

-

cinnamyl alcohol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.714

-

nerol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.741

-

linalool

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

1.212

-

nerol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

1.333

-

linalool

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

2.222

-

3,7-dimethyloct-6-en-1-ol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

10

-

3,7-dimethyloct-6-en-1-ol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

56000

-

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE

Persicaria minor

58000

-

1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

60000

-

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE

Persicaria minor

62000

-

1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

100000

-

isoform geraniol-DH I, gel filtration

Persicaria minor

120000

-

isoform geraniol-DH II, gel filtration

Persicaria minor

-

DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography

Persicaria minor

leaf

-

Persicaria minor

-

0.002

-

cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.175

-

isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.261

-

isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

beta-citronellol + NADP+

compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate

719124

Persicaria minor

?

-

?

borneol + NADP+

compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate

719124

Persicaria minor

?

-

?

carveol + NADP+

compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate

719124

Persicaria minor

?

-

?

cinnamyl alcohol + NADP+

compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate

719124

Persicaria minor

?

-

?

geraniol + NADP+

100% activity

719124

Persicaria minor

geranial + NADPH + H+

-

?

linalool + NADP+

compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate

719124

Persicaria minor

?

-

?

additional information

tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol

719124

Persicaria minor

?

-

nerol + NADP+

compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate

719124

Persicaria minor

?

-

?

p-cumic alcohol + NADP+

compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate

719124

Persicaria minor

?

-

?

heterodimer

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

40

-

isoforms geraniol-DH I and geraniol-DH II

Persicaria minor

30

70

rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C

Persicaria minor

9

-

isoform geraniol-DH I

Persicaria minor

9.5

-

isoform geraniol-DH II

Persicaria minor

6

10

isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below

Persicaria minor

isoform geraniol-DH II, isoelectric focusing

-

3.5

Persicaria minor

isoform geraniol-DH I, isoelectric focusing

-

8.8

additional information

adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity

Persicaria minor

2,2'-dipyridyl

isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM

Persicaria minor

cyanide

isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM

Persicaria minor

iodoacetamide

isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM

Persicaria minor

MgCl2

isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM

Persicaria minor

Sodium azide

isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM

Persicaria minor

0.021

-

NADP+

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.0588

-

NADP+

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.185

-

geraniol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.4

-

geraniol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.435

-

cinnamyl alcohol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.556

-

cinnamyl alcohol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.714

-

nerol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.741

-

linalool

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

1.212

-

nerol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

1.333

-

linalool

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

2.222

-

3,7-dimethyloct-6-en-1-ol

isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

10

-

3,7-dimethyloct-6-en-1-ol

isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

56000

-

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE

Persicaria minor

58000

-

1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

60000

-

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE

Persicaria minor

62000

-

1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

100000

-

isoform geraniol-DH I, gel filtration

Persicaria minor

120000

-

isoform geraniol-DH II, gel filtration

Persicaria minor

DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography

Persicaria minor

leaf

-

Persicaria minor

-

0.002

-

cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.175

-

isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

0.261

-

isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C

Persicaria minor

beta-citronellol + NADP+

compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate

719124

Persicaria minor

?

-

?

borneol + NADP+

compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate

719124

Persicaria minor

?

-

?

carveol + NADP+

compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate

719124

Persicaria minor

?

-

?

cinnamyl alcohol + NADP+

compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate

719124

Persicaria minor

?

-

?

geraniol + NADP+

100% activity

719124

Persicaria minor

geranial + NADPH + H+

-

?

linalool + NADP+

compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate

719124

Persicaria minor

?

-

?

additional information

tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol

719124

Persicaria minor

?

-

nerol + NADP+

compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate

719124

Persicaria minor

?

-

?

p-cumic alcohol + NADP+

compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate

719124

Persicaria minor

?

-

?

heterodimer

1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE

Persicaria minor

40

-

isoforms geraniol-DH I and geraniol-DH II

Persicaria minor

30

70

rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C

Persicaria minor

9

-

isoform geraniol-DH I

Persicaria minor

9.5

-

isoform geraniol-DH II

Persicaria minor

6

10

isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below

Persicaria minor

isoform geraniol-DH II, isoelectric focusing

-

3.5

Persicaria minor

isoform geraniol-DH I, isoelectric focusing

-

8.8

719124

Hassan

Monoterpene alcohol metabolism ...

Persicaria minor

Biosci. Biotechnol. Biochem.

76

1463-1470

2012

1

-

5

12

-

6

-

3

-

1

-

1

3

-

9

1

-

1

-

2

1

-

2

-

1

-

5

-

12

-

6

-

1

-

1

3

-

9

1

-

1

-

2

1

-

2

-

723856

Saito

Crystallization and preliminar ...

Backhousia citriodora

Acta Crystallogr. Sect. F

67

665-667

2011

-

1

-

2

-

3

-

1

-

1

-

1

-

2

-

1

-

724585

Yamaguchi

The effects of high hydrostati ...

Zingiber officinale

Biosci. Biotechnol. Biochem.

74

1981-1986

2010

-

1

-

2

-

1

-

686700

Noge

Geraniol dehydrogenase, the ke ...

Carpoglyphus lactis

FEBS J.

275

2807-2817

2008

-

1

-

2

-

1

-

3

-

1

-

1

-

4

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

4

1

-

1

-

667325

Iijima

Analysis of the enzymatic form ...

Ocimum basilicum

Arch. Biochem. Biophys.

448

141-149

2006

-

1

-

1

2

-

1

-

2

-

2

-

1

-

1

-

14

-

1

-

2

-

1

2

-

1

-

2

-

1

-

2

-

1

-

1

-

14

-

1

-

670514

Luan

Metabolism of geraniol in grap ...

Vitis vinifera

Phytochemistry

66

295-303

2005

-

2

-

1

-

2

-

3

-

2

-

2

-

2

-

2

-

3

-

286132

Sekiwa-Iijima

Geraniol dehydrogenase activit ...

Zingiber officinale

J. Agric. Food Chem.

49

5902-5906

2001

-

4

-

3

-

1

-

2

1

-

12

-

1

-

2

-

2

-

4

-

2

1

-

12

-

1

-

2

-

286131

Sangwan

-

Metabolism of acyclic monoterp ...

Cymbopogon flexuosus

J. Plant Physiol.

142

129-134

1993

-

3

4

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

2

-

3

-

4

-

1

-

1

-

1

-

1

-

1

-

286133

Singh

-

Oxidative pathways and essenti ...

Cymbopogon flexuosus

Plant Physiol. Biochem.

28

703-710

1990

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

286130

Potty

-

Oxidation of geraniol by enzym ...

Citrus sp.

Phytochemistry

9

1003-1007

1970

1

-

11

4

-

1

-

1

-

1

-

1

2

6

-

2

1

3

-

1

-

3

-

11

-

4

-

1

-

1

-

1

2

6

-

2

1

-

Monoterpene alcohol metabolism: identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from Polygonum minus leaves

Data extracted from this reference: