Data extracted from this reference:
Activating Compound
additional information
adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity
Persicaria minor
Inhibitors
2,2'-dipyridyl
isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM
Persicaria minor
cyanide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
iodoacetamide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
MgCl2
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM
Persicaria minor
Sodium azide
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM
Persicaria minor
KM Value [mM]
0.021
NADP+
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.0588
NADP+
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.185
geraniol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.4
geraniol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.435
cinnamyl alcohol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.556
cinnamyl alcohol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.714
nerol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.741
linalool
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
1.212
nerol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
1.333
linalool
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
2.222
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
10
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
Molecular Weight [Da]
56000
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
58000
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
60000
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
62000
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
100000
isoform geraniol-DH I, gel filtration
Persicaria minor
120000
isoform geraniol-DH II, gel filtration
Persicaria minor
Organism
Purification (Commentary)
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography
Persicaria minor
Source Tissue
Specific Activity [micromol/min/mg]
0.002
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.175
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.261
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
Substrates and Products (Substrate)
beta-citronellol + NADP+
compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate
719124
Persicaria minor
?
?
borneol + NADP+
compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate
719124
Persicaria minor
?
?
carveol + NADP+
compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate
719124
Persicaria minor
?
?
cinnamyl alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
?
geraniol + NADP+
100% activity
719124
Persicaria minor
geranial + NADPH + H+
?
linalool + NADP+
compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate
719124
Persicaria minor
?
?
additional information
tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol
719124
Persicaria minor
?
nerol + NADP+
compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
?
p-cumic alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate
719124
Persicaria minor
?
?
Subunits
heterodimer
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
Temperature Optimum [°C]
40
isoforms geraniol-DH I and geraniol-DH II
Persicaria minor
Temperature Stability [°C]
30
70
rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C
Persicaria minor
pH Optimum
9
isoform geraniol-DH I
Persicaria minor
9.5
isoform geraniol-DH II
Persicaria minor
pH Range
6
10
isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below
Persicaria minor
pI Value
Persicaria minor
isoform geraniol-DH II, isoelectric focusing
3.5
Persicaria minor
isoform geraniol-DH I, isoelectric focusing
8.8
Activating Compound (protein specific)
additional information
adding sodium metabisulfite, ascorbic acid, dithiothreitol, and EDTA to the extraction buffer and using Tris-HCl (pH 7.5) or potassium phosphate (pH 7.5) fails to enhance enzyme activity
Persicaria minor
Inhibitors (protein specific)
2,2'-dipyridyl
isoform geraniol-DH I shows 72% residual activity and geraniol-DH II shows 75% residual activity at 1 mM
Persicaria minor
cyanide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
iodoacetamide
isoforms geraniol-DH I and geraniol-DH II are completely inhibited at 1 mM
Persicaria minor
MgCl2
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 45% residual activity at 5 mM
Persicaria minor
Sodium azide
isoform geraniol-DH I shows 61% residual activity and geraniol-DH II shows 90% residual activity at 1 mM
Persicaria minor
KM Value [mM] (protein specific)
0.021
NADP+
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.0588
NADP+
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.185
geraniol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.4
geraniol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.435
cinnamyl alcohol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.556
cinnamyl alcohol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.714
nerol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.741
linalool
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
1.212
nerol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
1.333
linalool
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
2.222
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH II, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
10
3,7-dimethyloct-6-en-1-ol
isoform geraniol-DH I, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
Molecular Weight [Da] (protein specific)
56000
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
58000
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
60000
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE
Persicaria minor
62000
1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
100000
isoform geraniol-DH I, gel filtration
Persicaria minor
120000
isoform geraniol-DH II, gel filtration
Persicaria minor
Purification (Commentary) (protein specific)
DEAE Toyopearl 650M column chromatography, phenyl Toyopearl 650 M column chromatography, and AF-Red Toyopearl 650ML column chromatography
Persicaria minor
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
0.002
cell free extract, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.175
isoform geraniol-DH I, after 116.7fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
0.261
isoform geraniol-DH II, after 173.8fold purification, in 100 mM glycine-NaOH, pH 9.5, at 37°C
Persicaria minor
Substrates and Products (Substrate) (protein specific)
beta-citronellol + NADP+
compared to geraniol, isoform geraniol-DH I shows 72% activity and isoform geraniol-DH II shows 63% activity with this substrate
719124
Persicaria minor
?
?
borneol + NADP+
compared to geraniol, isoform geraniol-DH I shows 52% activity and isoform geraniol-DH II shows 29% activity with this substrate
719124
Persicaria minor
?
?
carveol + NADP+
compared to geraniol, isoform geraniol-DH I shows 44% activity with this substrate
719124
Persicaria minor
?
?
cinnamyl alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 60% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
?
geraniol + NADP+
100% activity
719124
Persicaria minor
geranial + NADPH + H+
?
linalool + NADP+
compared to geraniol, isoform geraniol-DH I shows 42% activity and isoform geraniol-DH II shows 92% activity with this substrate
719124
Persicaria minor
?
?
additional information
tetrahydrogeraniol, tetrahydrolinalool, menthol, benzyl alcohol, and farnesol are no substrates for both isoforms. Isoform geraniol-DH II does not react with 3,7-dihydrolinalool, and isoform geraniol-DH I does not react with carveol
719124
Persicaria minor
?
nerol + NADP+
compared to geraniol, isoform geraniol-DH I shows 64% activity and isoform geraniol-DH II shows 50% activity with this substrate
719124
Persicaria minor
?
?
p-cumic alcohol + NADP+
compared to geraniol, isoform geraniol-DH I shows 56% activity and isoform geraniol-DH II shows 46% activity with this substrate
719124
Persicaria minor
?
?
Subunits (protein specific)
heterodimer
1 * 56000 + 1 * 60000, isoform geraniol-DH II, SDS-PAGE; 1 * 58000 + 1 * 62000, isoform geraniol-DH I, SDS-PAGE
Persicaria minor
Temperature Optimum [°C] (protein specific)
40
isoforms geraniol-DH I and geraniol-DH II
Persicaria minor
Temperature Stability [°C] (protein specific)
30
70
rapid inactiviation of isoform geraniol-DH I occurs from 30-60°C with 60% activity at 40°C and 40% residual activity at 60°C. At 70°C the isoform shows less than 20% residual activity. Isoform geraniol-DH II retains over 80% activity after 10 min at 40°C, while from 30-50°C the activity decreases slightly and is lost at temperatures above 50°C
Persicaria minor
pH Optimum (protein specific)
9
isoform geraniol-DH I
Persicaria minor
9.5
isoform geraniol-DH II
Persicaria minor
pH Range (protein specific)
6
10
isoforms geraniol-DH I and geraniol-DH II show less than 40% activity below pH 6.5. Isoform geraniol-DH II shows no activity at pH 10.0, while isoform geraniol-DH I shows about 50% activity. Both isoforms are inactive at pH 6.0 and below
Persicaria minor
pI Value (protein specific)
Persicaria minor
isoform geraniol-DH II, isoelectric focusing
3.5
Persicaria minor
isoform geraniol-DH I, isoelectric focusing
8.8
Other publictions for EC 1.1.1.183
740786
Zhou
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Two types of alcohol dehydroge ...
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3
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13
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7
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4
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20
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3
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3
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4
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3
4
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13
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1
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4
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20
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3
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-
3
-
-
-
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12
12
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741134
Iijima
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Biosynthesis of geranial, a po ...
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Plant Biotechnol.
31
525-534
2014
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-
1
-
-
-
-
3
-
1
-
4
-
2
-
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-
4
-
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31
1
1
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3
1
1
-
3
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1
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-
2
4
-
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-
6
-
1
-
4
-
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-
5
-
-
31
1
1
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-
6
2
2
-
1
-
2
2
-
3
6
740553
Wany
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Isolation and in silico charac ...
Ocimum americanum
Int. J. Pharm. Bio. Sci.
4
B365-B379
2013
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1
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2
2
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719124
Hassan
Monoterpene alcohol metabolism ...
Persicaria minor
Biosci. Biotechnol. Biochem.
76
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2012
1
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5
12
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6
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3
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1
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1
3
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9
1
1
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1
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1
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1
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5
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12
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6
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1
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3
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9
1
1
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1
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2
1
-
2
-
-
-
-
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-
723856
Saito
Crystallization and preliminar ...
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Acta Crystallogr. Sect. F
67
665-667
2011
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1
1
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2
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1
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1
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724585
Yamaguchi
The effects of high hydrostati ...
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Biosci. Biotechnol. Biochem.
74
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2010
-
1
-
-
-
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2
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686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
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1
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2
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1
1
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1
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1
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4
1
1
1
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1
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1
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1
1
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2
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1
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1
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1
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4
1
1
1
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1
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667325
Iijima
Analysis of the enzymatic form ...
Ocimum basilicum
Arch. Biochem. Biophys.
448
141-149
2006
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1
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1
2
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1
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2
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2
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1
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1
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14
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1
1
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1
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1
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1
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1
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14
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1
1
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670514
Luan
Metabolism of geraniol in grap ...
Vitis vinifera
Phytochemistry
66
295-303
2005
-
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-
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2
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1
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2
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2
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2
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3
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286132
Sekiwa-Iijima
Geraniol dehydrogenase activit ...
Zingiber officinale
J. Agric. Food Chem.
49
5902-5906
2001
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4
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3
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1
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1
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12
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1
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2
2
2
2
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2
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4
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2
1
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12
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1
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2
2
2
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-
-
-
-
-
-
286131
Sangwan
-
Metabolism of acyclic monoterp ...
Cymbopogon flexuosus
J. Plant Physiol.
142
129-134
1993
-
-
-
-
-
-
3
4
-
-
1
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1
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1
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1
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1
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-
1
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2
-
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2
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4
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1
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1
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1
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1
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1
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-
-
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-
-
-
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286133
Singh
-
Oxidative pathways and essenti ...
Cymbopogon flexuosus
Plant Physiol. Biochem.
28
703-710
1990
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1
1
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1
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1
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1
1
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1
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1
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1
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-
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-
286130
Potty
-
Oxidation of geraniol by enzym ...
Citrus sp.
Phytochemistry
9
1003-1007
1970
1
-
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11
4
-
1
1
1
-
1
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-
1
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1
2
2
6
-
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-
2
1
1
3
-
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1
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3
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-
11
-
4
-
1
1
1
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-
-
1
-
1
2
2
6
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-