BRENDA - Enzyme Database show
show all sequences of 1.1.1.183

Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae)

Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.; FEBS J. 275, 2807-2817 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Carpoglyphus lactis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
geraniol
pH 9.0, 25°C
Carpoglyphus lactis
0.0596
-
NAD+
pH 9.0, 25°C
Carpoglyphus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42800
-
x * 42800, SDS-PAGE
Carpoglyphus lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NAD+
Carpoglyphus lactis
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone
geranial + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Carpoglyphus lactis
B2NI94
astigmatid mite
-
Purification (Commentary)
Commentary
Organism
native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview
Carpoglyphus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
31
-
purified enzyme
Carpoglyphus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
E,E-farnesol + NAD+
-
686700
Carpoglyphus lactis
?
-
-
-
?
geraniol + NAD+
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone
686700
Carpoglyphus lactis
geranial + NADH
-
-
-
?
geraniol + NAD+
stereospecific reaction, no activity with nerol
686700
Carpoglyphus lactis
geranial + NADH
-
-
-
?
additional information
substrate specificity, poor activity with citronellol, 3-methyl-2-buten-1-ol, 1-octanol, 1-butanol, 2-buten-1-ol, and ethanol, overview
686700
Carpoglyphus lactis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 42800, SDS-PAGE
Carpoglyphus lactis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Carpoglyphus lactis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
60
temprature profile, overview
Carpoglyphus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Carpoglyphus lactis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
specific for, no activity with NADP+
Carpoglyphus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Carpoglyphus lactis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
specific for, no activity with NADP+
Carpoglyphus lactis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
geraniol
pH 9.0, 25°C
Carpoglyphus lactis
0.0596
-
NAD+
pH 9.0, 25°C
Carpoglyphus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42800
-
x * 42800, SDS-PAGE
Carpoglyphus lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NAD+
Carpoglyphus lactis
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone
geranial + NADH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview
Carpoglyphus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
31
-
purified enzyme
Carpoglyphus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
E,E-farnesol + NAD+
-
686700
Carpoglyphus lactis
?
-
-
-
?
geraniol + NAD+
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone
686700
Carpoglyphus lactis
geranial + NADH
-
-
-
?
geraniol + NAD+
stereospecific reaction, no activity with nerol
686700
Carpoglyphus lactis
geranial + NADH
-
-
-
?
additional information
substrate specificity, poor activity with citronellol, 3-methyl-2-buten-1-ol, 1-octanol, 1-butanol, 2-buten-1-ol, and ethanol, overview
686700
Carpoglyphus lactis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42800, SDS-PAGE
Carpoglyphus lactis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Carpoglyphus lactis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
60
temprature profile, overview
Carpoglyphus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Carpoglyphus lactis
Other publictions for EC 1.1.1.183
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719124
Hassan
Monoterpene alcohol metabolism ...
Persicaria minor
Biosci. Biotechnol. Biochem.
76
1463-1470
2012
1
-
-
-
-
-
5
12
-
-
6
-
-
3
-
-
1
-
-
1
3
-
9
1
1
-
1
-
2
1
-
-
-
2
-
1
-
-
-
-
-
-
-
5
-
12
-
-
6
-
-
-
-
1
-
1
3
-
9
1
1
-
1
-
2
1
-
2
-
-
-
-
-
-
723856
Saito
Crystallization and preliminar ...
Backhousia citriodora
Acta Crystallogr. Sect. F
67
665-667
2011
-
-
1
1
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724585
Yamaguchi
The effects of high hydrostati ...
Zingiber officinale
Biosci. Biotechnol. Biochem.
74
1981-1986
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
1
-
-
-
-
2
-
-
1
1
-
3
-
-
1
-
-
-
1
-
4
1
1
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
667325
Iijima
Analysis of the enzymatic form ...
Ocimum basilicum
Arch. Biochem. Biophys.
448
141-149
2006
-
-
1
-
-
-
1
2
-
1
-
2
-
2
-
-
1
-
-
1
-
-
14
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
1
-
2
-
-
-
1
-
1
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
670514
Luan
Metabolism of geraniol in grap ...
Vitis vinifera
Phytochemistry
66
295-303
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286132
Sekiwa-Iijima
Geraniol dehydrogenase activit ...
Zingiber officinale
J. Agric. Food Chem.
49
5902-5906
2001
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
1
-
2
1
-
12
-
1
-
-
-
2
2
2
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
1
-
12
-
1
-
-
-
2
2
2
-
-
-
-
-
-
-
286131
Sangwan
-
Metabolism of acyclic monoterp ...
Cymbopogon flexuosus
J. Plant Physiol.
142
129-134
1993
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
4
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286133
Singh
-
Oxidative pathways and essenti ...
Cymbopogon flexuosus
Plant Physiol. Biochem.
28
703-710
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286130
Potty
-
Oxidation of geraniol by enzym ...
Citrus sp.
Phytochemistry
9
1003-1007
1970
1
-
-
-
-
-
11
4
-
1
1
1
-
1
-
-
1
-
-
1
2
2
6
-
-
-
-
-
2
1
1
3
-
-
-
1
-
-
3
-
-
-
-
11
-
4
-
1
1
1
-
-
-
1
-
1
2
2
6
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-