BRENDA - Enzyme Database show
show all sequences of 1.1.1.183

Analysis of the enzymatic formation of citral in the glands of sweet basil

Iijima, Y.; Wang, G.; Fridman, E.; Pichersky, E.; Arch. Biochem. Biophys. 448, 141-149 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli
Ocimum basilicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Zn2+
66% inhibition at 5 mM
Ocimum basilicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
geraniol
pH 9.5
Ocimum basilicum
0.037
-
nerol
pH 9.5
Ocimum basilicum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not affected by Na+, K+, NH4+, Ca2+, and Mg2+
Ocimum basilicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NADP+
Ocimum basilicum
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview
geranial + NADPH
-
-
ir
nerol + NADP+
Ocimum basilicum
-
neral + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ocimum basilicum
Q2KNL6
GEDH1; cv. Sweet Dani, gene GEDH1
-
Purification (Commentary)
Commentary
Organism
recombinant GEDH1 from Escherichia coli by anion exchange chromatography
Ocimum basilicum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
highest activity in young leaves decreasing to 60% in older leaves
Ocimum basilicum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-methyl-2-buten-1-ol + NADP+
22.7% of the activity with geraniol
667325
Ocimum basilicum
3-methyl-2-butenal + NADPH
-
-
-
?
3-methyl-3-buten-1-ol + NADP+
6.5% of the activity with geraniol
667325
Ocimum basilicum
3-methyl-3-butenal + NADPH
-
-
-
?
benzylalcohol + NADP+
5.5% of the activity with geraniol
667325
Ocimum basilicum
benzaldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NADP+
53.0% of the activity with geraniol
667325
Ocimum basilicum
cinnamyl aldehyde + NADPH
-
-
-
?
cis-3-hexenol + NADP+
13.3% of the activity with geraniol
667325
Ocimum basilicum
cis-3-hexenal + NADPH
-
-
-
?
citronellol + NADP+
16.6% of the activity with geraniol
667325
Ocimum basilicum
citronellal + NADPH
-
-
-
r
geraniol + NADP+
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview
667325
Ocimum basilicum
geranial + NADPH
-
-
-
ir
geraniol + NADP+
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo
667325
Ocimum basilicum
geranial + NADPH
-
-
-
?
hexanol + NADP+
31.6% of the activity with geraniol
667325
Ocimum basilicum
hexanal + NADPH
-
-
-
?
additional information
no activity with coniferyl alcohol, menthol, carveol, dihydrocarveol, 3,7-dimethyl-octanol, ethanol, butanol, and isobutanol
667325
Ocimum basilicum
?
-
-
-
-
nerol + NADP+
-
667325
Ocimum basilicum
neral + NADPH
-
-
-
r
nerol + NADP+
101% of the activity with geraniol
667325
Ocimum basilicum
neral + NADPH
-
-
-
r
phenylethanol + NADP+
10.2% of the activity with geraniol
667325
Ocimum basilicum
phenylethanal + NADPH
-
-
-
?
phenylpropanol + NADP+
22.1% of the activity with geraniol
667325
Ocimum basilicum
phenylpropanal + NADPH
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Ocimum basilicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8.5
10
over 72% of maximal activity at pH 8.5 and pH 10.0
Ocimum basilicum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ocimum basilicum
NADPH
-
Ocimum basilicum
Cloned(Commentary) (protein specific)
Commentary
Organism
GEDH1, phylogenetic tree and analysis, sequence comparison, expression in Escherichia coli
Ocimum basilicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ocimum basilicum
NADPH
-
Ocimum basilicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Zn2+
66% inhibition at 5 mM
Ocimum basilicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
geraniol
pH 9.5
Ocimum basilicum
0.037
-
nerol
pH 9.5
Ocimum basilicum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme is not affected by Na+, K+, NH4+, Ca2+, and Mg2+
Ocimum basilicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NADP+
Ocimum basilicum
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview
geranial + NADPH
-
-
ir
nerol + NADP+
Ocimum basilicum
-
neral + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GEDH1 from Escherichia coli by anion exchange chromatography
Ocimum basilicum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
highest activity in young leaves decreasing to 60% in older leaves
Ocimum basilicum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-methyl-2-buten-1-ol + NADP+
22.7% of the activity with geraniol
667325
Ocimum basilicum
3-methyl-2-butenal + NADPH
-
-
-
?
3-methyl-3-buten-1-ol + NADP+
6.5% of the activity with geraniol
667325
Ocimum basilicum
3-methyl-3-butenal + NADPH
-
-
-
?
benzylalcohol + NADP+
5.5% of the activity with geraniol
667325
Ocimum basilicum
benzaldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NADP+
53.0% of the activity with geraniol
667325
Ocimum basilicum
cinnamyl aldehyde + NADPH
-
-
-
?
cis-3-hexenol + NADP+
13.3% of the activity with geraniol
667325
Ocimum basilicum
cis-3-hexenal + NADPH
-
-
-
?
citronellol + NADP+
16.6% of the activity with geraniol
667325
Ocimum basilicum
citronellal + NADPH
-
-
-
r
geraniol + NADP+
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo, the geraniol,/nerol pathway involves sveral dehydrogenases, overview
667325
Ocimum basilicum
geranial + NADPH
-
-
-
ir
geraniol + NADP+
the back reaction is catalyzed by CAD1, the cinnamyl alcohol dehydrogenase in vivo
667325
Ocimum basilicum
geranial + NADPH
-
-
-
?
hexanol + NADP+
31.6% of the activity with geraniol
667325
Ocimum basilicum
hexanal + NADPH
-
-
-
?
additional information
no activity with coniferyl alcohol, menthol, carveol, dihydrocarveol, 3,7-dimethyl-octanol, ethanol, butanol, and isobutanol
667325
Ocimum basilicum
?
-
-
-
-
nerol + NADP+
-
667325
Ocimum basilicum
neral + NADPH
-
-
-
r
nerol + NADP+
101% of the activity with geraniol
667325
Ocimum basilicum
neral + NADPH
-
-
-
r
phenylethanol + NADP+
10.2% of the activity with geraniol
667325
Ocimum basilicum
phenylethanal + NADPH
-
-
-
?
phenylpropanol + NADP+
22.1% of the activity with geraniol
667325
Ocimum basilicum
phenylpropanal + NADPH
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Ocimum basilicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8.5
10
over 72% of maximal activity at pH 8.5 and pH 10.0
Ocimum basilicum
Other publictions for EC 1.1.1.183
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719124
Hassan
Monoterpene alcohol metabolism ...
Persicaria minor
Biosci. Biotechnol. Biochem.
76
1463-1470
2012
1
-
-
-
-
-
5
12
-
-
6
-
-
3
-
-
1
-
-
1
3
-
9
1
1
-
1
-
2
1
-
-
-
2
-
1
-
-
-
-
-
-
-
5
-
12
-
-
6
-
-
-
-
1
-
1
3
-
9
1
1
-
1
-
2
1
-
2
-
-
-
-
-
-
723856
Saito
Crystallization and preliminar ...
Backhousia citriodora
Acta Crystallogr. Sect. F
67
665-667
2011
-
-
1
1
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724585
Yamaguchi
The effects of high hydrostati ...
Zingiber officinale
Biosci. Biotechnol. Biochem.
74
1981-1986
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
1
-
-
-
-
2
-
-
1
1
-
3
-
-
1
-
-
-
1
-
4
1
1
1
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
667325
Iijima
Analysis of the enzymatic form ...
Ocimum basilicum
Arch. Biochem. Biophys.
448
141-149
2006
-
-
1
-
-
-
1
2
-
1
-
2
-
2
-
-
1
-
-
1
-
-
14
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
1
-
2
-
-
-
1
-
1
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
670514
Luan
Metabolism of geraniol in grap ...
Vitis vinifera
Phytochemistry
66
295-303
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286132
Sekiwa-Iijima
Geraniol dehydrogenase activit ...
Zingiber officinale
J. Agric. Food Chem.
49
5902-5906
2001
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
1
-
2
1
-
12
-
1
-
-
-
2
2
2
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
1
-
12
-
1
-
-
-
2
2
2
-
-
-
-
-
-
-
286131
Sangwan
-
Metabolism of acyclic monoterp ...
Cymbopogon flexuosus
J. Plant Physiol.
142
129-134
1993
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
4
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286133
Singh
-
Oxidative pathways and essenti ...
Cymbopogon flexuosus
Plant Physiol. Biochem.
28
703-710
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286130
Potty
-
Oxidation of geraniol by enzym ...
Citrus sp.
Phytochemistry
9
1003-1007
1970
1
-
-
-
-
-
11
4
-
1
1
1
-
1
-
-
1
-
-
1
2
2
6
-
-
-
-
-
2
1
1
3
-
-
-
1
-
-
3
-
-
-
-
11
-
4
-
1
1
1
-
-
-
1
-
1
2
2
6
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-