BRENDA - Enzyme Database show
show all sequences of 1.1.1.183

Oxidation of geraniol by enzyme system from orange

Potty, V.H.; Bruemmer, J.H.; Phytochemistry 9, 1003-1007 (1970)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
protects enzyme from inhibition
Citrus sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
(+)-limonene
100% inhibition at 0.488 mM
Citrus sp.
8-hydroxyquinoline
50% decrease at 0.67 mM
Citrus sp.
alpha,alpha'-dipyridyl
50% inhibition at 25 mM
Citrus sp.
alpha-terpineol
about 55% inhibition at 0.56 mM
Citrus sp.
EDTA
50% inhibition at 67.7 mM
Citrus sp.
iodoacetamide
-
Citrus sp.
linalool
about 55% inhibition at 1.12 mM
Citrus sp.
o-phenanthroline
50% inhibition at 16.7 mM
Citrus sp.
oxidized glutathione
reaction completely stopped at 3.333 mM
Citrus sp.
Sodium azide
50% inhibition at 83 mM
Citrus sp.
Zn2+
50% inhibition 1.67 mM
Citrus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0465
-
geraniol
pH 9.0
Citrus sp.
0.0833
-
geraniol
pH 7.0
Citrus sp.
0.454
-
NADP+
pH 9.0
Citrus sp.
0.83
-
NADP+
pH 7.0
Citrus sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
suggested to be part of the enzyme
Citrus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
92000
-
estimation, gel filtration
Citrus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NADP+
Citrus sp.
key intermediate in terpenoid biosynthesis
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus sp.
-
orange
-
Purification (Commentary)
Commentary
Organism
partially, ammonium sulfate fractionation, gel filtration, anion exchange chromatography
Citrus sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrus sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
nerol shows 60% of the rate with geraniol, farnesol about 11% and citronellol 8%
Citrus sp.
0.203
-
-
Citrus sp.
Storage Stability
Storage Stability
Organism
-19°C, dialyzed, rapid loss of activity
Citrus sp.
-96°C, undialyzed ammonium sulfate fraction, 6 months, quite active
Citrus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citronellol + NADP+
-
286130
Citrus sp.
citronellal + NADPH
-
286130
Citrus sp.
?
farnesol + NADP+
-
286130
Citrus sp.
farnesal + NADPH
-
286130
Citrus sp.
?
geraniol + NADP+
-
286130
Citrus sp.
geranial NADPH
-
286130
Citrus sp.
r
geraniol + NADP+
key intermediate in terpenoid biosynthesis
286130
Citrus sp.
?
-
-
-
?
additional information
nerol, farnesol and citronellol are oxidized at slower rates
286130
Citrus sp.
?
-
-
-
-
nerol + NADP+
-
286130
Citrus sp.
neral + NADPH
-
286130
Citrus sp.
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
geranial reduction
Citrus sp.
9
-
geraniol oxidation
Citrus sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
below 5 complete and irreversible inactivation after 5 min
Citrus sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
below pH 5 complete and irreversible inactivation after 5 min
Citrus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NAD+ is ineffective
Citrus sp.
NADP+
-
Citrus sp.
NADPH
-
Citrus sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
protects enzyme from inhibition
Citrus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NAD+ is ineffective
Citrus sp.
NADP+
-
Citrus sp.
NADPH
-
Citrus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(+)-limonene
100% inhibition at 0.488 mM
Citrus sp.
8-hydroxyquinoline
50% decrease at 0.67 mM
Citrus sp.
alpha,alpha'-dipyridyl
50% inhibition at 25 mM
Citrus sp.
alpha-terpineol
about 55% inhibition at 0.56 mM
Citrus sp.
EDTA
50% inhibition at 67.7 mM
Citrus sp.
iodoacetamide
-
Citrus sp.
linalool
about 55% inhibition at 1.12 mM
Citrus sp.
o-phenanthroline
50% inhibition at 16.7 mM
Citrus sp.
oxidized glutathione
reaction completely stopped at 3.333 mM
Citrus sp.
Sodium azide
50% inhibition at 83 mM
Citrus sp.
Zn2+
50% inhibition 1.67 mM
Citrus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0465
-
geraniol
pH 9.0
Citrus sp.
0.0833
-
geraniol
pH 7.0
Citrus sp.
0.454
-
NADP+
pH 9.0
Citrus sp.
0.83
-
NADP+
pH 7.0
Citrus sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
suggested to be part of the enzyme
Citrus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
92000
-
estimation, gel filtration
Citrus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geraniol + NADP+
Citrus sp.
key intermediate in terpenoid biosynthesis
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially, ammonium sulfate fractionation, gel filtration, anion exchange chromatography
Citrus sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
nerol shows 60% of the rate with geraniol, farnesol about 11% and citronellol 8%
Citrus sp.
0.203
-
-
Citrus sp.
Storage Stability (protein specific)
Storage Stability
Organism
-19°C, dialyzed, rapid loss of activity
Citrus sp.
-96°C, undialyzed ammonium sulfate fraction, 6 months, quite active
Citrus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citronellol + NADP+
-
286130
Citrus sp.
citronellal + NADPH
-
286130
Citrus sp.
?
farnesol + NADP+
-
286130
Citrus sp.
farnesal + NADPH
-
286130
Citrus sp.
?
geraniol + NADP+
-
286130
Citrus sp.
geranial NADPH
-
286130
Citrus sp.
r
geraniol + NADP+
key intermediate in terpenoid biosynthesis
286130
Citrus sp.
?
-
-
-
?
additional information
nerol, farnesol and citronellol are oxidized at slower rates
286130
Citrus sp.
?
-
-
-
-
nerol + NADP+
-
286130
Citrus sp.
neral + NADPH
-
286130
Citrus sp.
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
geranial reduction
Citrus sp.
9
-
geraniol oxidation
Citrus sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
below 5 complete and irreversible inactivation after 5 min
Citrus sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
below pH 5 complete and irreversible inactivation after 5 min
Citrus sp.
Other publictions for EC 1.1.1.183
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719124
Hassan
Monoterpene alcohol metabolism ...
Persicaria minor
Biosci. Biotechnol. Biochem.
76
1463-1470
2012
1
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5
12
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6
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3
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1
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1
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9
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1
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1
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2
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1
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5
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12
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6
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1
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1
3
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9
1
1
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1
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2
1
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2
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-
723856
Saito
Crystallization and preliminar ...
Backhousia citriodora
Acta Crystallogr. Sect. F
67
665-667
2011
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1
1
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-
-
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2
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3
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1
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1
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1
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724585
Yamaguchi
The effects of high hydrostati ...
Zingiber officinale
Biosci. Biotechnol. Biochem.
74
1981-1986
2010
-
1
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2
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686700
Noge
Geraniol dehydrogenase, the ke ...
Carpoglyphus lactis
FEBS J.
275
2807-2817
2008
-
-
1
-
-
-
-
2
-
-
1
1
-
3
-
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1
-
-
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1
-
4
1
1
1
-
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1
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1
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1
1
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-
-
2
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1
1
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1
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-
1
-
4
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
667325
Iijima
Analysis of the enzymatic form ...
Ocimum basilicum
Arch. Biochem. Biophys.
448
141-149
2006
-
-
1
-
-
-
1
2
-
1
-
2
-
2
-
-
1
-
-
1
-
-
14
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
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1
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2
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1
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1
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-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
670514
Luan
Metabolism of geraniol in grap ...
Vitis vinifera
Phytochemistry
66
295-303
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286132
Sekiwa-Iijima
Geraniol dehydrogenase activit ...
Zingiber officinale
J. Agric. Food Chem.
49
5902-5906
2001
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
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1
-
2
1
-
12
-
1
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-
-
2
2
2
2
-
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2
-
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4
-
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-
-
2
1
-
12
-
1
-
-
-
2
2
2
-
-
-
-
-
-
-
286131
Sangwan
-
Metabolism of acyclic monoterp ...
Cymbopogon flexuosus
J. Plant Physiol.
142
129-134
1993
-
-
-
-
-
-
3
4
-
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1
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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286133
Singh
-
Oxidative pathways and essenti ...
Cymbopogon flexuosus
Plant Physiol. Biochem.
28
703-710
1990
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1
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1
1
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1
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1
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1
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1
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1
1
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1
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1
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1
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-
-
-
-
286130
Potty
-
Oxidation of geraniol by enzym ...
Citrus sp.
Phytochemistry
9
1003-1007
1970
1
-
-
-
-
-
11
4
-
1
1
1
-
1
-
-
1
-
-
1
2
2
6
-
-
-
-
-
2
1
1
3
-
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1
-
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3
-
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-
11
-
4
-
1
1
1
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1
-
1
2
2
6
-
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-
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2
1
1
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