BRENDA - Enzyme Database show
show all sequences of 1.1.1.181

Purification and properties of a 3beta-hydroxy-DELTA5-C27-steroid oxidoreductase from rabbit liver microsomes

Wikvall, K.; J. Biol. Chem. 256, 3376-3380 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
FMN
30% inhibition at 0.1 mM
Oryctolagus cuniculus
Quinacrine
slight inhibition at 1 mM
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
cholest-5-ene-3beta,7alpha-diol
-
Oryctolagus cuniculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholest-5-ene-3beta,7alpha-diol + NAD+
Oryctolagus cuniculus
highly specific
7alpha-hydroxycholest-4-en-3-one + NADH
-
Oryctolagus cuniculus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
rabbit
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.23
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholest-5-ene-3beta,7alpha-diol + NAD+
highly specific
286129
Oryctolagus cuniculus
7alpha-hydroxycholest-4-en-3-one + NADH
-
286129
Oryctolagus cuniculus
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 46000, SDS-PAGE
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
in potassium phosphate buffer
Oryctolagus cuniculus
9
-
in Tris-HCl buffer
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
FMN
30% inhibition at 0.1 mM
Oryctolagus cuniculus
Quinacrine
slight inhibition at 1 mM
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
cholest-5-ene-3beta,7alpha-diol
-
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholest-5-ene-3beta,7alpha-diol + NAD+
Oryctolagus cuniculus
highly specific
7alpha-hydroxycholest-4-en-3-one + NADH
-
Oryctolagus cuniculus
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.23
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholest-5-ene-3beta,7alpha-diol + NAD+
highly specific
286129
Oryctolagus cuniculus
7alpha-hydroxycholest-4-en-3-one + NADH
-
286129
Oryctolagus cuniculus
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 46000, SDS-PAGE
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
in potassium phosphate buffer
Oryctolagus cuniculus
9
-
in Tris-HCl buffer
Oryctolagus cuniculus
Other publictions for EC 1.1.1.181
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
656364
Cheng
Molecular genetics of 3beta-hy ...
Homo sapiens
J. Clin. Endocrinol. Metab.
88
1833-1841
2003
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1
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2
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286129
Wikvall
Purification and properties of ...
Oryctolagus cuniculus
J. Biol. Chem.
256
3376-3380
1981
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2
1
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1
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1
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1
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1
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1
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