BRENDA - Enzyme Database show
show all sequences of 1.1.1.179

D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii

Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schoenheit, P.; J. Biol. Chem. 284, 27290-27303 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
induced by D-xylose
Haloferax volcanii
Cloned(Commentary)
Commentary
Organism
cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain; expression in Escherichia coli
Haloferax volcanii
Engineering
Amino acid exchange
Commentary
Organism
additional information
in-frame deletion mutant can not grow on xylose but grows unaffected on glucose as the wild-type
Haloferax volcanii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
-
Haloferax volcanii
0.36
-
NAD+
-
Haloferax volcanii
0.75
-
NADP+
pH 8.3, 42°C
Haloferax volcanii
4.7
-
D-xylose
-
Haloferax volcanii
8
9
D-xylose
pH 8.3, 42°C
Haloferax volcanii
198
-
D-glucose
-
Haloferax volcanii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5550
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
42300
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
165000
-
native enzyme
Haloferax volcanii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NADP+
Haloferax volcanii
initial step in xylose degradation
D-xylono-1,5-lactone + NADPH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii DS2
initial step in xylose degradation
D-xylono-1,5-lactone + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloferax volcanii
D4GP29
DS70 strain H26
-
Haloferax volcanii
D4GP30
-
-
Haloferax volcanii DS2
D4GP30
-
-
Haloferax volcanii GR501
D4GP29
DS70 strain H26
-
Purification (Commentary)
Commentary
Organism
on Ni-NTA column and by gel filtration
Haloferax volcanii
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:xylose-grown cell
-
Haloferax volcanii
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.8
-
with D-glucose as substrate
Haloferax volcanii
10
-
pH 8.3, 42°C
Haloferax volcanii
24.1
-
with D-xylose as substrate
Haloferax volcanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NAD(P)+
-
698940
Haloferax volcanii
D-gluconolactone + NAD(P)H
-
-
-
?
D-glucose + NAD(P)+
-
698940
Haloferax volcanii GR501
D-gluconolactone + NAD(P)H
-
-
-
?
D-glucose + NADP+
20% of the activity with D-xylose
698940
Haloferax volcanii
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
20% of the activity with D-xylose
698940
Haloferax volcanii DS2
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NAD(P)+
is highly specific for D-xylose
698940
Haloferax volcanii
D-xylonolactone + NAD(P)H
-
-
-
?
D-xylose + NAD(P)+
is highly specific for D-xylose
698940
Haloferax volcanii GR501
D-xylonolactone + NAD(P)H
-
-
-
?
D-xylose + NADP+
-
698940
Haloferax volcanii
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
698940
Haloferax volcanii
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
-
698940
Haloferax volcanii DS2
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
698940
Haloferax volcanii DS2
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
additional information
no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
698940
Haloferax volcanii
?
-
-
-
-
additional information
no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
698940
Haloferax volcanii GR501
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Haloferax volcanii
NADP+
no activity with NAD+
Haloferax volcanii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
induced by D-xylose
Haloferax volcanii
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain
Haloferax volcanii
expression in Escherichia coli
Haloferax volcanii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Haloferax volcanii
NADP+
no activity with NAD+
Haloferax volcanii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
in-frame deletion mutant can not grow on xylose but grows unaffected on glucose as the wild-type
Haloferax volcanii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
-
Haloferax volcanii
0.36
-
NAD+
-
Haloferax volcanii
0.75
-
NADP+
pH 8.3, 42°C
Haloferax volcanii
4.7
-
D-xylose
-
Haloferax volcanii
8
9
D-xylose
pH 8.3, 42°C
Haloferax volcanii
198
-
D-glucose
-
Haloferax volcanii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5550
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
42300
-
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
165000
-
native enzyme
Haloferax volcanii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NADP+
Haloferax volcanii
initial step in xylose degradation
D-xylono-1,5-lactone + NADPH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii DS2
initial step in xylose degradation
D-xylono-1,5-lactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
on Ni-NTA column and by gel filtration
Haloferax volcanii
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:xylose-grown cell
-
Haloferax volcanii
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.8
-
with D-glucose as substrate
Haloferax volcanii
10
-
pH 8.3, 42°C
Haloferax volcanii
24.1
-
with D-xylose as substrate
Haloferax volcanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NAD(P)+
-
698940
Haloferax volcanii
D-gluconolactone + NAD(P)H
-
-
-
?
D-glucose + NAD(P)+
-
698940
Haloferax volcanii GR501
D-gluconolactone + NAD(P)H
-
-
-
?
D-glucose + NADP+
20% of the activity with D-xylose
698940
Haloferax volcanii
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
20% of the activity with D-xylose
698940
Haloferax volcanii DS2
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NAD(P)+
is highly specific for D-xylose
698940
Haloferax volcanii
D-xylonolactone + NAD(P)H
-
-
-
?
D-xylose + NAD(P)+
is highly specific for D-xylose
698940
Haloferax volcanii GR501
D-xylonolactone + NAD(P)H
-
-
-
?
D-xylose + NADP+
-
698940
Haloferax volcanii
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
698940
Haloferax volcanii
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
-
698940
Haloferax volcanii DS2
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
698940
Haloferax volcanii DS2
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
additional information
no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
698940
Haloferax volcanii
?
-
-
-
-
additional information
no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
698940
Haloferax volcanii GR501
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis
Haloferax volcanii
Other publictions for EC 1.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723749
Mihasan
Evidence of a plasmid-encoded ...
Paenarthrobacter nicotinovorans
Res. Microbiol.
164
22-30
2013
-
-
1
-
-
-
-
3
-
1
1
-
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
3
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724150
Ahmed
-
Oxidation and reduction of D-x ...
Ogataea angusta
Aust. J. Basic Appl. Sci.
5
95-100
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
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-
-
2
-
-
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-
-
-
-
-
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-
2
-
-
-
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-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725867
Nygard
Bioconversion of d-xylose to d ...
Trichoderma reesei
Metab. Eng.
13
383-391
2011
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698940
Johnsen
D-xylose degradation pathway i ...
Haloferax volcanii, Haloferax volcanii DS2, Haloferax volcanii GR501
J. Biol. Chem.
284
27290-27303
2009
1
-
1
-
1
-
-
6
-
-
3
2
-
4
-
-
1
-
-
1
3
-
12
1
-
-
-
-
-
-
-
2
-
-
-
1
-
2
2
-
1
-
-
-
-
6
-
-
3
2
-
-
-
1
-
1
3
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684150
Carbone
Structure of monkey dimeric di ...
Macaca fascicularis
Acta Crystallogr. Sect. D
64
532-542
2008
-
-
1
1
15
-
2
13
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
8
1
-
-
1
-
-
-
-
-
1
1
1
15
-
-
2
-
13
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-
686797
Berghaell
Identification in the mould Hy ...
Trichoderma reesei
FEMS Microbiol. Lett.
277
249-253
2007
-
-
1
-
-
-
-
4
-
1
1
1
-
1
-
-
1
-
-
-
1
-
6
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
1
1
1
-
-
-
1
-
-
1
-
6
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
655884
Johnsen
Novel xylose dehydrogenase in ...
Haloarcula marismortui
J. Bacteriol.
186
6198-6207
2004
-
-
1
-
-
-
-
3
-
3
2
1
-
1
-
-
1
-
-
1
1
-
5
1
2
1
-
3
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
3
2
1
-
-
-
1
-
1
1
-
5
1
2
1
-
3
1
1
-
-
-
-
-
-
3
3
286128
Aoki
Identity of dimeric dihydrodio ...
Canis lupus familiaris, Homo sapiens, Macaca fuscata, Oryctolagus cuniculus, Sus scrofa
Chem. Biol. Interact.
130-132
775-784
2001
4
-
-
-
2
-
1
4
-
-
-
5
-
10
-
-
4
1
-
8
2
-
18
-
-
-
-
-
8
-
-
7
-
-
-
4
-
-
7
-
2
-
-
1
-
4
-
-
-
5
-
-
-
4
-
8
2
-
18
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
286127
Asada
Roles of His-79 and Tyr-180 of ...
Macaca fuscata
Biochem. Biophys. Res. Commun.
278
333-337
2000
1
-
1
-
2
-
1
9
-
-
2
-
-
1
-
-
1
-
-
1
3
-
3
1
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
1
-
9
-
-
2
-
-
-
-
1
-
1
3
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286126
Zepeda
NADP(+)-dependent D-xylose deh ...
Sus scrofa
Biochem. J.
266
637-644
1990
-
-
-
-
-
-
2
9
-
-
2
-
-
1
-
-
1
1
-
1
1
-
7
1
1
-
-
7
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
9
-
-
2
-
-
-
-
1
-
1
1
-
7
1
1
-
-
7
1
-
-
-
-
-
-
-
-
-
286124
Wissler
-
D-Xylose:NADP+ oxidoreductase ...
Bos taurus, Canis lupus familiaris, Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
358
1300-1301
1977
-
-
-
-
-
-
-
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-
3
-
-
3
-
-
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-
7
-
-
24
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
7
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286125
Suzuki
Oxidation and reduction of D-x ...
Starmera quercuum
Appl. Microbiol.
25
850-852
1973
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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-
-
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-
-
-
-
-
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1
-
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-
-
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-
-