BRENDA - Enzyme Database show
show all sequences of 1.1.1.179

Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui

Johnsen, U.; Schonheit, P.; J. Bacteriol. 186, 6198-6207 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Haloarcula marismortui
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
2.3
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
64
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
maximal activity at 1.5 M
Haloarcula marismortui
MgCl2
maximal activity at 100 M
Haloarcula marismortui
NaCl
maximal activity at 1.5 M
Haloarcula marismortui
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39900
-
4 * 39900, SDS-PAGE
Haloarcula marismortui
175000
-
gel filtration
Haloarcula marismortui
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NADP+
Haloarcula marismortui
initial step in xylose degradation
D-xylonolactone + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloarcula marismortui
Q5UY95
-
-
Purification (Commentary)
Commentary
Organism
-
Haloarcula marismortui
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:xylose-grown cell
-
Haloarcula marismortui
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
100
-
pH 8.8, 37C
Haloarcula marismortui
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NADP+
reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
655884
Haloarcula marismortui
D-gluconolactone + NADPH + H+
-
-
-
?
D-ribose + NADP+
reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
655884
Haloarcula marismortui
D-ribonolactone + NADPH + H+
-
-
-
?
D-xylose + NAD+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
655884
Haloarcula marismortui
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
655884
Haloarcula marismortui
D-xylonolactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
655884
Haloarcula marismortui
D-xylonolactone + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 39900, SDS-PAGE
Haloarcula marismortui
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Haloarcula marismortui
50
-
-
Haloarcula marismortui
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
50
activity increases exponentially with temperature between 25C and 45C. 50C: optimum
Haloarcula marismortui
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
273
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
356
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
360
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Haloarcula marismortui
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
more than 50% of maximal activity
Haloarcula marismortui
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Haloarcula marismortui
NADP+
preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Haloarcula marismortui
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Haloarcula marismortui
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Haloarcula marismortui
NADP+
preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
Haloarcula marismortui
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
2.3
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
64
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
maximal activity at 1.5 M
Haloarcula marismortui
MgCl2
maximal activity at 100 M
Haloarcula marismortui
NaCl
maximal activity at 1.5 M
Haloarcula marismortui
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39900
-
4 * 39900, SDS-PAGE
Haloarcula marismortui
175000
-
gel filtration
Haloarcula marismortui
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NADP+
Haloarcula marismortui
initial step in xylose degradation
D-xylonolactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Haloarcula marismortui
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:xylose-grown cell
-
Haloarcula marismortui
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
100
-
pH 8.8, 37C
Haloarcula marismortui
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NADP+
reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
655884
Haloarcula marismortui
D-gluconolactone + NADPH + H+
-
-
-
?
D-ribose + NADP+
reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP*
655884
Haloarcula marismortui
D-ribonolactone + NADPH + H+
-
-
-
?
D-xylose + NAD+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
655884
Haloarcula marismortui
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NADP+
initial step in xylose degradation
655884
Haloarcula marismortui
D-xylonolactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+
655884
Haloarcula marismortui
D-xylonolactone + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 39900, SDS-PAGE
Haloarcula marismortui
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Haloarcula marismortui
50
-
-
Haloarcula marismortui
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
50
activity increases exponentially with temperature between 25C and 45C. 50C: optimum
Haloarcula marismortui
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
273
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
356
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
360
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Haloarcula marismortui
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
more than 50% of maximal activity
Haloarcula marismortui
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.043
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
1.57
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
2.97
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.043
-
D-glucose
pH 8.3, 37C
Haloarcula marismortui
1.57
-
D-ribose
pH 8.3, 37C
Haloarcula marismortui
2.97
-
D-xylose
pH 8.3, 37C
Haloarcula marismortui
Other publictions for EC 1.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723749
Mihasan
Evidence of a plasmid-encoded ...
Paenarthrobacter nicotinovorans
Res. Microbiol.
164
22-30
2013
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1
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3
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1
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3
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1
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3
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3
3
724150
Ahmed
-
Oxidation and reduction of D-x ...
Ogataea angusta
Aust. J. Basic Appl. Sci.
5
95-100
2011
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2
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1
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725867
Nygard
Bioconversion of d-xylose to d ...
Trichoderma reesei
Metab. Eng.
13
383-391
2011
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1
1
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1
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1
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1
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698940
Johnsen
D-xylose degradation pathway i ...
Haloferax volcanii, Haloferax volcanii DS2, Haloferax volcanii GR501
J. Biol. Chem.
284
27290-27303
2009
1
-
1
-
1
-
-
6
-
-
3
2
-
4
-
-
1
-
-
1
3
-
12
1
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2
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1
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2
2
-
1
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6
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3
2
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1
-
1
3
-
12
1
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-
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-
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-
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-
684150
Carbone
Structure of monkey dimeric di ...
Macaca fascicularis
Acta Crystallogr. Sect. D
64
532-542
2008
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1
1
15
-
2
13
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2
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2
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1
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2
1
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8
1
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1
1
1
15
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2
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13
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2
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1
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2
1
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8
1
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686797
Berghaell
Identification in the mould Hy ...
Trichoderma reesei
FEMS Microbiol. Lett.
277
249-253
2007
-
-
1
-
-
-
-
4
-
1
1
1
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1
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1
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1
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6
1
1
-
-
2
1
-
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1
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1
1
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-
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4
-
1
1
1
-
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1
-
-
1
-
6
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
655884
Johnsen
Novel xylose dehydrogenase in ...
Haloarcula marismortui
J. Bacteriol.
186
6198-6207
2004
-
-
1
-
-
-
-
3
-
3
2
1
-
1
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1
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1
1
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5
1
2
1
-
3
1
1
-
2
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1
2
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3
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3
2
1
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1
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1
1
-
5
1
2
1
-
3
1
1
-
-
-
-
-
-
3
3
286128
Aoki
Identity of dimeric dihydrodio ...
Canis lupus familiaris, Homo sapiens, Macaca fuscata, Oryctolagus cuniculus, Sus scrofa
Chem. Biol. Interact.
130-132
775-784
2001
4
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2
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1
4
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5
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10
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4
1
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8
2
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18
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8
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7
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7
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2
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1
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4
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4
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8
2
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18
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8
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286127
Asada
Roles of His-79 and Tyr-180 of ...
Macaca fuscata
Biochem. Biophys. Res. Commun.
278
333-337
2000
1
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1
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2
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1
9
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2
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1
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1
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3
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3
1
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1
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1
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9
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2
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1
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1
3
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3
1
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286126
Zepeda
NADP(+)-dependent D-xylose deh ...
Sus scrofa
Biochem. J.
266
637-644
1990
-
-
-
-
-
-
2
9
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2
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1
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1
1
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1
1
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7
1
1
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7
1
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2
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2
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2
-
9
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2
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1
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1
1
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7
1
1
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7
1
-
-
-
-
-
-
-
-
-
286124
Wissler
-
D-Xylose:NADP+ oxidoreductase ...
Bos taurus, Canis lupus familiaris, Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
358
1300-1301
1977
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3
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7
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24
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3
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3
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3
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7
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24
-
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286125
Suzuki
Oxidation and reduction of D-x ...
Starmera quercuum
Appl. Microbiol.
25
850-852
1973
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-
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1
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1
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1
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1
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1
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