BRENDA - Enzyme Database show
show all sequences of 1.1.1.179

NADP(+)-dependent D-xylose dehydrogenase from pig liver. Purification and properties

Zepeda, S.; Monasterio, O.; Ureta, T.; Biochem. J. 266, 637-644 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
L-Lyxose
-
Sus scrofa
NAD+
-
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADP+
-
Sus scrofa
6.5
-
D-xylose
-
Sus scrofa
8.8
-
D-xylose
-
Sus scrofa
27
-
D-ribose
-
Sus scrofa
49
-
D-glucose
-
Sus scrofa
54
-
2-deoxy-D-glucose
-
Sus scrofa
57
-
L-arabinose
-
Sus scrofa
130
-
D-arabinose
-
Sus scrofa
140
-
D-galactose
-
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Sus scrofa
62000
-
gel filtration
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Reaction
Reaction
Commentary
Organism
D-xylose + NADP+ = D-xylono-1,5-lactone + NADPH + H+
mechanism
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.1
-
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + NADP+
-
286126
Sus scrofa
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-galactose + NADP+
-
286126
Sus scrofa
D-galactono-1,5-lactone + NADPH
-
-
-
-
D-glucose + NADP+
-
286126
Sus scrofa
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-mannose + NADP+
-
286126
Sus scrofa
D-mannono-1,5-lactone + NADPH
-
-
-
?
D-ribose + NADP+
-
286126
Sus scrofa
?
-
-
-
?
D-xylose + NADP+
-
286126
Sus scrofa
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
L-arabinose + NADP+
-
286126
Sus scrofa
L-arabinono-1,4-lactone + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sus scrofa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9
-
D-arabinose
-
Sus scrofa
12
-
D-galactose
-
Sus scrofa
16
-
D-glucose
-
Sus scrofa
22
-
2-deoxy-D-glucose
-
Sus scrofa
40
-
D-ribose
-
Sus scrofa
45
-
L-arabinose
-
Sus scrofa
58
-
D-xylose
-
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
not: NAD+
Sus scrofa
NADP+
-
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
not: NAD+
Sus scrofa
NADP+
-
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-Lyxose
-
Sus scrofa
NAD+
-
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADP+
-
Sus scrofa
6.5
-
D-xylose
-
Sus scrofa
8.8
-
D-xylose
-
Sus scrofa
27
-
D-ribose
-
Sus scrofa
49
-
D-glucose
-
Sus scrofa
54
-
2-deoxy-D-glucose
-
Sus scrofa
57
-
L-arabinose
-
Sus scrofa
130
-
D-arabinose
-
Sus scrofa
140
-
D-galactose
-
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Sus scrofa
62000
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.1
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-D-glucose + NADP+
-
286126
Sus scrofa
2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-galactose + NADP+
-
286126
Sus scrofa
D-galactono-1,5-lactone + NADPH
-
-
-
-
D-glucose + NADP+
-
286126
Sus scrofa
D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-mannose + NADP+
-
286126
Sus scrofa
D-mannono-1,5-lactone + NADPH
-
-
-
?
D-ribose + NADP+
-
286126
Sus scrofa
?
-
-
-
?
D-xylose + NADP+
-
286126
Sus scrofa
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
L-arabinose + NADP+
-
286126
Sus scrofa
L-arabinono-1,4-lactone + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sus scrofa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9
-
D-arabinose
-
Sus scrofa
12
-
D-galactose
-
Sus scrofa
16
-
D-glucose
-
Sus scrofa
22
-
2-deoxy-D-glucose
-
Sus scrofa
40
-
D-ribose
-
Sus scrofa
45
-
L-arabinose
-
Sus scrofa
58
-
D-xylose
-
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Sus scrofa
Other publictions for EC 1.1.1.179
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723749
Mihasan
Evidence of a plasmid-encoded ...
Paenarthrobacter nicotinovorans
Res. Microbiol.
164
22-30
2013
-
-
1
-
-
-
-
3
-
1
1
-
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
3
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
3
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
724150
Ahmed
-
Oxidation and reduction of D-x ...
Ogataea angusta
Aust. J. Basic Appl. Sci.
5
95-100
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725867
Nygard
Bioconversion of d-xylose to d ...
Trichoderma reesei
Metab. Eng.
13
383-391
2011
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698940
Johnsen
D-xylose degradation pathway i ...
Haloferax volcanii, Haloferax volcanii DS2, Haloferax volcanii GR501
J. Biol. Chem.
284
27290-27303
2009
1
-
1
-
1
-
-
6
-
-
3
2
-
4
-
-
1
-
-
1
3
-
12
1
-
-
-
-
-
-
-
2
-
-
-
1
-
2
2
-
1
-
-
-
-
6
-
-
3
2
-
-
-
1
-
1
3
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684150
Carbone
Structure of monkey dimeric di ...
Macaca fascicularis
Acta Crystallogr. Sect. D
64
532-542
2008
-
-
1
1
15
-
2
13
-
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
8
1
-
-
1
-
-
-
-
-
1
1
1
15
-
-
2
-
13
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-
686797
Berghaell
Identification in the mould Hy ...
Trichoderma reesei
FEMS Microbiol. Lett.
277
249-253
2007
-
-
1
-
-
-
-
4
-
1
1
1
-
1
-
-
1
-
-
-
1
-
6
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
1
1
1
-
-
-
1
-
-
1
-
6
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
655884
Johnsen
Novel xylose dehydrogenase in ...
Haloarcula marismortui
J. Bacteriol.
186
6198-6207
2004
-
-
1
-
-
-
-
3
-
3
2
1
-
1
-
-
1
-
-
1
1
-
5
1
2
1
-
3
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
3
2
1
-
-
-
1
-
1
1
-
5
1
2
1
-
3
1
1
-
-
-
-
-
-
3
3
286128
Aoki
Identity of dimeric dihydrodio ...
Canis lupus familiaris, Homo sapiens, Macaca fuscata, Oryctolagus cuniculus, Sus scrofa
Chem. Biol. Interact.
130-132
775-784
2001
4
-
-
-
2
-
1
4
-
-
-
5
-
10
-
-
4
1
-
8
2
-
18
-
-
-
-
-
8
-
-
7
-
-
-
4
-
-
7
-
2
-
-
1
-
4
-
-
-
5
-
-
-
4
-
8
2
-
18
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
286127
Asada
Roles of His-79 and Tyr-180 of ...
Macaca fuscata
Biochem. Biophys. Res. Commun.
278
333-337
2000
1
-
1
-
2
-
1
9
-
-
2
-
-
1
-
-
1
-
-
1
3
-
3
1
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
1
-
9
-
-
2
-
-
-
-
1
-
1
3
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286126
Zepeda
NADP(+)-dependent D-xylose deh ...
Sus scrofa
Biochem. J.
266
637-644
1990
-
-
-
-
-
-
2
9
-
-
2
-
-
1
-
-
1
1
-
1
1
-
7
1
1
-
-
7
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
9
-
-
2
-
-
-
-
1
-
1
1
-
7
1
1
-
-
7
1
-
-
-
-
-
-
-
-
-
286124
Wissler
-
D-Xylose:NADP+ oxidoreductase ...
Bos taurus, Canis lupus familiaris, Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
358
1300-1301
1977
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
7
-
-
24
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
7
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286125
Suzuki
Oxidation and reduction of D-x ...
Starmera quercuum
Appl. Microbiol.
25
850-852
1973
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-