BRENDA - Enzyme Database show
show all sequences of 1.1.1.178

D- and L-leucine metabolism and regulation of their pathways in Pseudomonas putida

Conrad R.S.; Massey L.K.; Sokatch J.R.; J. Bacteriol. 4, 103-111 (1974)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
2-methyl-3-hydroxybutyryl-CoA
-
Pseudomonas putida
0.5
-
3-hydroxybutyryl-CoA
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
130000
-
gel filtration, sucrose density gradient centrifugation
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methyl-3-hydroxybutyryl-CoA + NAD+
Pseudomonas putida
isoleucine metabolism
2-methylacetoacetyl-CoA + NADH
-
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
Pseudomonas putida PpG2
isoleucine metabolism
2-methylacetoacetyl-CoA + NADH
-
Pseudomonas putida PpG2
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
PpG2
-
Pseudomonas putida PpG2
-
PpG2
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH + H+
inducible by tiglate
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.2
-
2-methyl-3-hydroxybutyryl-CoA
Pseudomonas putida
21.7
-
3-hydroxybutyryl-CoA
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
286120
Pseudomonas putida
3-methyl-2-oxopentanoyl-CoA + NADH
-
-
-
?
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
3-methyl-2-oxopentanoyl-CoA + NADH
-
-
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
isoleucine metabolism
286120
Pseudomonas putida
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida PpG2
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
isoleucine metabolism
286120
Pseudomonas putida PpG2
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida PpG2
?
3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida
acetoacetyl-CoA + NADH
-
-
-
?
3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
acetoacetyl-CoA + NADH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
2-methyl-3-hydroxybutyryl-CoA
-
Pseudomonas putida
0.5
-
3-hydroxybutyryl-CoA
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
130000
-
gel filtration, sucrose density gradient centrifugation
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-methyl-3-hydroxybutyryl-CoA + NAD+
Pseudomonas putida
isoleucine metabolism
2-methylacetoacetyl-CoA + NADH
-
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
Pseudomonas putida PpG2
isoleucine metabolism
2-methylacetoacetyl-CoA + NADH
-
Pseudomonas putida PpG2
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.2
-
2-methyl-3-hydroxybutyryl-CoA
Pseudomonas putida
21.7
-
3-hydroxybutyryl-CoA
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
286120
Pseudomonas putida
3-methyl-2-oxopentanoyl-CoA + NADH
-
-
-
?
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
3-methyl-2-oxopentanoyl-CoA + NADH
-
-
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
isoleucine metabolism
286120
Pseudomonas putida
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida PpG2
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
isoleucine metabolism
286120
Pseudomonas putida PpG2
2-methylacetoacetyl-CoA + NADH
-
286120
Pseudomonas putida PpG2
?
3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida
acetoacetyl-CoA + NADH
-
-
-
?
3-hydroxybutyryl-CoA + NAD+
-
286120
Pseudomonas putida PpG2
acetoacetyl-CoA + NADH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas putida
Other publictions for EC 1.1.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725097
Yang
A 5-methylcytosine hotspot res ...
Homo sapiens
Gene
515
380-384
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
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-
-
-
711851
Rauschenberger
A non-enzymatic function of 17 ...
Homo sapiens, Mus musculus, Xenopus laevis
EMBO Mol. Med.
2
51-62
2010
-
1
-
-
4
-
-
-
3
-
-
-
-
6
-
-
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-
-
3
-
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-
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-
-
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-
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1
-
-
-
4
-
-
-
-
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3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
697369
Garcia-Villoria
Study of patients and carriers ...
Homo sapiens
Clin. Biochem.
42
27-33
2009
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
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1
-
-
-
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670477
Garcia-Villoria
2-Methyl-3-hydroxybutyryl-CoA ...
Homo sapiens
Pediatr. Res.
58
488-491
2005
-
-
-
-
4
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286123
Zschocke
Progressive infantile neurodeg ...
Homo sapiens
Pediatr. Res.
48
852-855
2000
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286122
Luo
Short-chain 3-hydroxy-2-methyl ...
Rattus norvegicus
Arch. Biochem. Biophys.
321
214-220
1995
-
-
-
-
-
-
-
-
1
-
2
1
-
3
-
-
1
-
-
2
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
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1
-
2
1
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286121
Gibson
A coupled assay detecting defe ...
Homo sapiens
Clin. Chim. Acta
205
127-135
1992
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286120
Conrad R.S.; Massey L.K.; Sokatch J.R.
D- and L-leucine metabolism an ...
Pseudomonas putida, Pseudomonas putida PpG2
J. Bacteriol.
4
103-111
1974
-
-
-
-
-
-
-
2
-
-
1
2
-
3
-
-
1
1
-
-
2
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
2
-
-
-
1
-
-
2
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-