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show all sequences of 1.1.1.177

Structural and functional analysis of the gpsA gene product of Archaeoglobus fulgidus: A glycerol-3-phosphate dehydrogenase with an unusual NADP+ preference

Sakasegawa, S.I.; Hagemeier, C.H.; Thauer, R.K.; Essen, L.O.; Shima, S.; Protein Sci. 13, 3161-3171 (2004)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Archaeoglobus fulgidus
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glyceraldehyde 3-phosphate
-
Archaeoglobus fulgidus
L-alpha-glycerol 3-phosphate
-
Archaeoglobus fulgidus
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADPH
pH 6.6, 70C
Archaeoglobus fulgidus
0.1
-
L-alpha-glycerol 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
0.8
-
NADP+
pH 6.6, 70C
Archaeoglobus fulgidus
1
-
D-glyceraldehyde 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Archaeoglobus fulgidus
-
recombinant enzyme
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4
-
pH 6.6, 70C, substrate L-alpha-glycerol 3-phosphate
Archaeoglobus fulgidus
44
-
pH 6.6, 70C, substrate D-glyceraldehyde 3-phosphate
Archaeoglobus fulgidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + NADPH
-
657307
Archaeoglobus fulgidus
L-alpha-glycerol 3-phosphate + NADP+
-
-
-
r
L-alpha-glycerol 3-phosphate + NADP+
-
657307
Archaeoglobus fulgidus
D-glyceraldehyde 3-phosphate + NADPH
-
-
-
r
additional information
no enzymic activity with dihydroxyacetone, glycerol, glycerol-2-phosphate, D-glyceraldehyde-3-phosphate, DL-glyceraldehyde, D-erythrose-4-phosphate, D-fructose-4-phosphate
657307
Archaeoglobus fulgidus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
crystallization data
Archaeoglobus fulgidus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Archaeoglobus fulgidus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
85
-
30 min, in presence of 200 mM potassium phosphate or 100 mM NaCl or 50 mM ammonium sulfate, completely stable
Archaeoglobus fulgidus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
or below, reduction reaction
Archaeoglobus fulgidus
9.5
-
or above, oxidation reaction
Archaeoglobus fulgidus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
KI-value for NADPH is below 0.005 mM
Archaeoglobus fulgidus
0.005
-
NADP+
pH 6.6, 70C
Archaeoglobus fulgidus
0.1
-
D-glyceraldehyde 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
17
-
L-alpha-glycerol 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Archaeoglobus fulgidus
deduced from gene sequence
-
5
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Archaeoglobus fulgidus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glyceraldehyde 3-phosphate
-
Archaeoglobus fulgidus
L-alpha-glycerol 3-phosphate
-
Archaeoglobus fulgidus
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
KI-value for NADPH is below 0.005 mM
Archaeoglobus fulgidus
0.005
-
NADP+
pH 6.6, 70C
Archaeoglobus fulgidus
0.1
-
D-glyceraldehyde 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
17
-
L-alpha-glycerol 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADPH
pH 6.6, 70C
Archaeoglobus fulgidus
0.1
-
L-alpha-glycerol 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
0.8
-
NADP+
pH 6.6, 70C
Archaeoglobus fulgidus
1
-
D-glyceraldehyde 3-phosphate
pH 6.6, 70C
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4
-
pH 6.6, 70C, substrate L-alpha-glycerol 3-phosphate
Archaeoglobus fulgidus
44
-
pH 6.6, 70C, substrate D-glyceraldehyde 3-phosphate
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + NADPH
-
657307
Archaeoglobus fulgidus
L-alpha-glycerol 3-phosphate + NADP+
-
-
-
r
L-alpha-glycerol 3-phosphate + NADP+
-
657307
Archaeoglobus fulgidus
D-glyceraldehyde 3-phosphate + NADPH
-
-
-
r
additional information
no enzymic activity with dihydroxyacetone, glycerol, glycerol-2-phosphate, D-glyceraldehyde-3-phosphate, DL-glyceraldehyde, D-erythrose-4-phosphate, D-fructose-4-phosphate
657307
Archaeoglobus fulgidus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
crystallization data
Archaeoglobus fulgidus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Archaeoglobus fulgidus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
85
-
30 min, in presence of 200 mM potassium phosphate or 100 mM NaCl or 50 mM ammonium sulfate, completely stable
Archaeoglobus fulgidus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
or below, reduction reaction
Archaeoglobus fulgidus
9.5
-
or above, oxidation reaction
Archaeoglobus fulgidus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Archaeoglobus fulgidus
deduced from gene sequence
-
5
Other publictions for EC 1.1.1.177
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657307
Sakasegawa
Structural and functional anal ...
Archaeoglobus fulgidus
Protein Sci.
13
3161-3171
2004
-
-
-
1
-
-
4
4
-
-
-
-
-
1
-
-
-
-
-
-
2
-
3
1
1
-
1
-
2
-
-
2
4
1
-
-
-
-
2
1
-
-
-
4
4
4
-
-
-
-
-
-
-
-
-
-
2
-
3
1
1
-
1
-
2
-
-
1
-
-
-
-
-
-
286119
Glushankov
Pentose phosphate pathway of c ...
Rattus sp.
Biokhimiya
41
1788-1790
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-