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show all sequences of 1.1.1.176

Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid

Fossati, E.; Polentini, F.; Carrea, G.; Riva, S.; Biotechnol. Bioeng. 93, 1216-1220 (2006)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetone
at 25% v/v and above
Clostridium sp.
additional information
no substrate inhibition at high concentration of cholic acid
Clostridium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium sp.
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
commercial preparation
enzyme in 50% glycerol-containing solution
Clostridium sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview
Clostridium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholic acid + NADP+
the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview
668040
Clostridium sp.
12-ketochenodeoxycholic acid + NADPH
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Clostridium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Clostridium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetone
at 25% v/v and above
Clostridium sp.
additional information
no substrate inhibition at high concentration of cholic acid
Clostridium sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
commercial preparation
enzyme in 50% glycerol-containing solution
Clostridium sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview
Clostridium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholic acid + NADP+
the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview
668040
Clostridium sp.
12-ketochenodeoxycholic acid + NADPH
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Clostridium sp.
Other publictions for EC 1.1.1.176
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701240
Giovannini
7alpha- and 12alpha-Hydroxyste ...
Acinetobacter lwoffii
Steroids
73
1385-1390
2008
-
1
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1
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1
1
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668040
Fossati
Exploitation of the alcohol de ...
Clostridium sp.
Biotechnol. Bioeng.
93
1216-1220
2006
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2
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1
1
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1
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1
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347747
Panichajakul
-
Purification and characterizat ...
Brevibacterium fuscum
Microb. Util. Renewable Resour.
8
119-128
1993
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2
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1
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1
1
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2
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347748
Panichajakul
-
Bile acids induction of 12 alp ...
Brevibacterium fuscum
Microb. Util. Renewable Resour.
8
112-118
1993
-
-
-
-
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1
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1
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2
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347737
Braun
12 alpha-hydroxysteroid dehydr ...
Clostridium sp.
Eur. J. Biochem.
196
439-450
1991
-
-
-
-
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1
5
5
-
-
3
-
-
1
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1
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-
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2
6
1
1
-
2
-
2
-
-
3
-
-
-
-
-
-
3
-
-
1
-
5
-
5
-
-
3
-
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-
-
1
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-
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2
6
1
1
-
2
-
2
-
-
-
-
-
-
-
-
-
286074
Ottolina
Enzymatic synthesis of [4R-2H] ...
Clostridium sp.
Biochim. Biophys. Acta
998
173-178
1989
-
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1
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2
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286075
Kinoshita
-
Purification and properties of ...
Brevibacterium fuscum
J. Ferment. Technol.
66
145-152
1988
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1
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1
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6
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1
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6
-
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347739
MacDonald
Affinity chromatography of bil ...
Clostridium sp.
J. Chromatogr.
259
154-158
1983
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1
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1
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347740
MacDonald
Bile salt 3alpha- and 12alpha- ...
Eggerthella lenta
Appl. Environ. Microbiol.
37
992-1000
1979
-
-
-
-
-
-
-
5
-
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1
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1
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1
7
-
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1
1
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1
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1
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5
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1
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1
7
-
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1
1
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347741
Harris
Partial purification and chara ...
Clostridium leptum
Biochim. Biophys. Acta
528
148-157
1978
-
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6
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1
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2
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1
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1
6
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1
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1
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1
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1
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6
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1
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1
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1
6
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1
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1
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347742
Mahony
Bile salt degradation by nonfe ...
Clostridium sp.
Appl. Environ. Microbiol.
34
419-423
1977
-
-
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1
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1
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347743
MacDonald
NAD-dependent 3alpha- and 12al ...
Eggerthella lenta
Biochim. Biophys. Acta
489
466-476
1977
-
-
-
-
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2
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2
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1
1
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1
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1
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2
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1
1
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1
-
1
-
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-
-
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-
-
-
-
347744
MacDonald
3alpha-, 7alpha- and 12alpha-h ...
Clostridium perfringens
Biochim. Biophys. Acta
450
142-153
1976
-
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1
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3
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1
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4
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1
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1
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1
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1
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1
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4
-
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2
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1
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