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Literature summary for 1.1.1.176 extracted from

  • Harris, J.N.; Hylemon, P.B.
    Partial purification and characterization of NADP-dependent 12alpha-hydroxysteroid dehydrogenase from Clostridium leptum (1978), Biochim. Biophys. Acta, 528, 148-157.
    View publication on PubMed
Search for more literature for 1.1.1.176

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 3alpha,12alpha-dihydroxy-5beta-cholanoyl taurine
-
 [Clostridium] leptum
0.57
-
 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
 [Clostridium] leptum
1.6
-
 3alpha,12alpha-dihydroxy-5beta-cholanoate
-
 [Clostridium] leptum
2.7
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
 [Clostridium] leptum
4
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine
-
 [Clostridium] leptum
7
-
 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
 [Clostridium] leptum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
225000
-
 gel filtration [Clostridium] leptum

Organism

Organism UniProt Comment Textmining
[Clostridium] leptum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Clostridium] leptum

Storage Stability

Storage Stability Organism
-20°C, 20 days, 50% loss of activity [Clostridium] leptum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholic acid + NAD(P)+
-
 [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
taurocholic acid + NAD(P)+
-
 [Clostridium] leptum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
 ?
taurodeoxycholic acid + NAD+
-
 [Clostridium] leptum 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 4 half-life: 11 days [Clostridium] leptum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 oxidation of cholic acid [Clostridium] leptum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 [Clostridium] leptum