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show all sequences of 1.1.1.175

Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonate

Toivari, M.; Nygard, Y.; Kumpula, E.P.; Vehkomaeki, M.L.; Bencina, M.; Valkonen, M.; Maaheimo, H.; Andberg, M.; Koivula, A.; Ruohonen, L.; Penttilae, M.; Wiebe, M.G.; Metab. Eng. 14, 427-436 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0
Caulobacter vibrioides
Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Caulobacter vibrioides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
0.24
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
0.26
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
0.31
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
0.4
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
0.46
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
40
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
100
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
180
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NAD+
Caulobacter vibrioides
-
D-xylonolactone + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caulobacter vibrioides
B8H1Z0
putative
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylose + NAD+
-
725868
Caulobacter vibrioides
D-xylonolactone + NADH + H+
-
-
-
?
L-arabinose + NAD+
-
725868
Caulobacter vibrioides
L-arabinono-1,4-lactone + NADH + H+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22.3
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
22.7
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
24.2
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
25.8
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
26.2
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
29.2
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
30.3
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
31
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
35.7
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Caulobacter vibrioides
Application (protein specific)
Application
Commentary
Organism
synthesis
expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0
Caulobacter vibrioides
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Caulobacter vibrioides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Caulobacter vibrioides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
0.24
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
0.26
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
0.31
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
0.4
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
0.46
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
40
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
100
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
180
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-xylose + NAD+
Caulobacter vibrioides
-
D-xylonolactone + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-xylose + NAD+
-
725868
Caulobacter vibrioides
D-xylonolactone + NADH + H+
-
-
-
?
L-arabinose + NAD+
-
725868
Caulobacter vibrioides
L-arabinono-1,4-lactone + NADH + H+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
22.3
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
22.7
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
24.2
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
25.8
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
26.2
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
29.2
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
30.3
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
31
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
35.7
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.12
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
0.28
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
0.6
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
66.7
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
66.7
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
101.7
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
115
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
125
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
280
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.12
-
L-arabinose
25°C, pH 6.8
Caulobacter vibrioides
0.28
-
L-arabinose
25°C, pH 7.0
Caulobacter vibrioides
0.6
-
L-arabinose
25°C, pH 9.0
Caulobacter vibrioides
66.7
-
D-xylose
25°C, pH 6.8
Caulobacter vibrioides
66.7
-
NAD+
25°C, pH 9.0
Caulobacter vibrioides
101.7
-
NAD+
25°C, pH 6.8
Caulobacter vibrioides
115
-
NAD+
25°C, pH 7.0
Caulobacter vibrioides
125
-
D-xylose
25°C, pH 7.0
Caulobacter vibrioides
280
-
D-xylose
25°C, pH 9.0
Caulobacter vibrioides
Other publictions for EC 1.1.1.175
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725868
Toivari
Metabolic engineering of Sacch ...
Caulobacter vibrioides
Metab. Eng.
14
427-436
2012
-
1
1
-
-
-
-
9
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
687376
Stephens
Genetic analysis of a novel pa ...
Caulobacter vibrioides, Caulobacter vibrioides NA1000
J. Bacteriol.
189
2181-2185
2007
-
-
1
-
-
-
-
1
-
-
-
2
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286118
Mostad
The stereospecificity of hydro ...
Sus scrofa
Biochem. Biophys. Res. Commun.
233
681-686
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639098
Bonete
Glucose dehydrogenase from the ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
FEBS Lett.
383
227-229
1996
-
-
-
-
-
-
1
-
-
-
2
-
-
5
-
-
1
-
-
-
-
1
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
1
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
286117
Dahms
D-Xylose dehydrogenase ...
Pseudomonas sp., Pseudomonas sp. MSU-1
Methods Enzymol.
89
226-228
1982
-
-
-
-
-
1
-
2
-
-
-
2
-
8
-
-
1
-
-
1
1
1
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286115
Yamanaka
-
Purification and properties of ...
Pseudomonas sp.
Hakko Kogaku Kaishi
57
322-331
1979
-
-
-
-
-
-
-
7
-
-
1
5
-
1
-
-
1
-
-
1
2
-
5
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
7
-
-
1
5
-
-
-
1
-
1
2
-
5
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
286116
Yamanaka
-
A specific NAD-D-xylose dehydr ...
Arthrobacter sp., Arthrobacter sp. 588
Agric. Biol. Chem.
41
1493-1499
1977
-
-
-
-
-
-
-
1
-
-
1
2
-
2
-
-
1
-
-
1
1
-
2
-
1
-
2
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
1
1
-
2
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-