BRENDA - Enzyme Database show
show all sequences of 1.1.1.175

Purification and properties of D-xylose dehydrogenase in bacteria

Yamanaka, K.; Gino, M.; Hakko Kogaku Kaishi 57, 322-331 (1979)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
NAD+
strain 89B
Pseudomonas sp.
0.39
-
NAD+
strain 90A
Pseudomonas sp.
0.77
-
D-xylose
strain 90A
Pseudomonas sp.
2.9
-
D-xylose
strain 89B
Pseudomonas sp.
5.9
-
L-arabinose
strain 89B
Pseudomonas sp.
30
-
D-galactose
strain 89B
Pseudomonas sp.
100
-
L-arabinose
strain 90A
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NAD+
Pseudomonas sp.
low activity
? + NADH
-
Pseudomonas sp.
?
D-glucose + NAD+
Pseudomonas sp.
-
? + NADH
-
Pseudomonas sp.
?
D-lyxose + NAD+
Pseudomonas sp.
low activity
? + NADH
-
Pseudomonas sp.
?
D-xylose + NAD+
Pseudomonas sp.
-
D-xylonolactone + NADH + H+
-
Pseudomonas sp.
?
L-arabinose + NAD+
Pseudomonas sp.
-
? + NADH
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
strains 89B, 90A
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques, isoelectric focusing
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:D-xylose-grown cell
inducible enzyme synthesis by growth in D-xylose-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
30.9
-
strain 89B
Pseudomonas sp.
143
-
strain 90A
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + NAD+
low activity
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-glucose + NAD+
-
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-lyxose + NAD+
low activity
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-xylose + NAD+
-
286115
Pseudomonas sp.
D-xylonolactone + NADH + H+
-
286115
Pseudomonas sp.
?
L-arabinose + NAD+
-
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
55
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.3
9
stable within, strain 89B
Pseudomonas sp.
6
7.5
stable within, strain 90A
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
NAD+
strain 89B
Pseudomonas sp.
0.39
-
NAD+
strain 90A
Pseudomonas sp.
0.77
-
D-xylose
strain 90A
Pseudomonas sp.
2.9
-
D-xylose
strain 89B
Pseudomonas sp.
5.9
-
L-arabinose
strain 89B
Pseudomonas sp.
30
-
D-galactose
strain 89B
Pseudomonas sp.
100
-
L-arabinose
strain 90A
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galactose + NAD+
Pseudomonas sp.
low activity
? + NADH
-
Pseudomonas sp.
?
D-glucose + NAD+
Pseudomonas sp.
-
? + NADH
-
Pseudomonas sp.
?
D-lyxose + NAD+
Pseudomonas sp.
low activity
? + NADH
-
Pseudomonas sp.
?
D-xylose + NAD+
Pseudomonas sp.
-
D-xylonolactone + NADH + H+
-
Pseudomonas sp.
?
L-arabinose + NAD+
Pseudomonas sp.
-
? + NADH
-
Pseudomonas sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques, isoelectric focusing
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:D-xylose-grown cell
inducible enzyme synthesis by growth in D-xylose-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
30.9
-
strain 89B
Pseudomonas sp.
143
-
strain 90A
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galactose + NAD+
low activity
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-glucose + NAD+
-
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-lyxose + NAD+
low activity
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
D-xylose + NAD+
-
286115
Pseudomonas sp.
D-xylonolactone + NADH + H+
-
286115
Pseudomonas sp.
?
L-arabinose + NAD+
-
286115
Pseudomonas sp.
? + NADH
-
286115
Pseudomonas sp.
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
55
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
10
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.3
9
stable within, strain 89B
Pseudomonas sp.
6
7.5
stable within, strain 90A
Pseudomonas sp.
Other publictions for EC 1.1.1.175
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725868
Toivari
Metabolic engineering of Sacch ...
Caulobacter vibrioides
Metab. Eng.
14
427-436
2012
-
1
1
-
-
-
-
9
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
687376
Stephens
Genetic analysis of a novel pa ...
Caulobacter vibrioides, Caulobacter vibrioides NA1000
J. Bacteriol.
189
2181-2185
2007
-
-
1
-
-
-
-
1
-
-
-
2
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286118
Mostad
The stereospecificity of hydro ...
Sus scrofa
Biochem. Biophys. Res. Commun.
233
681-686
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639098
Bonete
Glucose dehydrogenase from the ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
FEBS Lett.
383
227-229
1996
-
-
-
-
-
-
1
-
-
-
2
-
-
5
-
-
1
-
-
-
-
1
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
1
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
286117
Dahms
D-Xylose dehydrogenase ...
Pseudomonas sp., Pseudomonas sp. MSU-1
Methods Enzymol.
89
226-228
1982
-
-
-
-
-
1
-
2
-
-
-
2
-
8
-
-
1
-
-
1
1
1
2
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
-
-
2
-
-
-
1
-
1
1
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286115
Yamanaka
-
Purification and properties of ...
Pseudomonas sp.
Hakko Kogaku Kaishi
57
322-331
1979
-
-
-
-
-
-
-
7
-
-
1
5
-
1
-
-
1
-
-
1
2
-
5
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
7
-
-
1
5
-
-
-
1
-
1
2
-
5
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
286116
Yamanaka
-
A specific NAD-D-xylose dehydr ...
Arthrobacter sp., Arthrobacter sp. 588
Agric. Biol. Chem.
41
1493-1499
1977
-
-
-
-
-
-
-
1
-
-
1
2
-
2
-
-
1
-
-
1
1
-
2
-
1
-
2
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
1
1
-
2
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-