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show all sequences of 1.1.1.172

Purification and properties of alpha-ketoadipate reductase, a newly discovered enzyme from human placenta

Suda, T.; Robinson, J.C.; Fjellstedt, T.A.; Arch. Biochem. Biophys. 176, 610-620 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NADH
uncompetitive inhibition at high concentrations
Homo sapiens
oxalate
10% inhibition at 0.5 mM
Homo sapiens
p-chloromercuribenzoate
slight inhibition at 0.02 mM, combined with 0.26 mM SDS
Homo sapiens
SDS
slight inhibition at 0.26 mM, combined with 0.02 mM p-chloromercuribenzoate
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
NADH
-
Homo sapiens
2.6
-
2-oxoadipate
-
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoadipate + NADH
Homo sapiens
highly specific
2-hydroxyadipate + NAD+
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
near homogeneity, chromatography techniques, electrofocusing
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Storage Stability
Storage Stability
Organism
4°C, 25 mM potassium phosphate, pH 7.5, 20% glycerol, no decrease of activity for several months
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoadipate + NADH
highly specific
286112
Homo sapiens
2-hydroxyadipate + NAD+
-
286112
Homo sapiens
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADH
uncompetitive inhibition at high concentrations
Homo sapiens
oxalate
10% inhibition at 0.5 mM
Homo sapiens
p-chloromercuribenzoate
slight inhibition at 0.02 mM, combined with 0.26 mM SDS
Homo sapiens
SDS
slight inhibition at 0.26 mM, combined with 0.02 mM p-chloromercuribenzoate
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
NADH
-
Homo sapiens
2.6
-
2-oxoadipate
-
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
95000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoadipate + NADH
Homo sapiens
highly specific
2-hydroxyadipate + NAD+
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
near homogeneity, chromatography techniques, electrofocusing
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 25 mM potassium phosphate, pH 7.5, 20% glycerol, no decrease of activity for several months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoadipate + NADH
highly specific
286112
Homo sapiens
2-hydroxyadipate + NAD+
-
286112
Homo sapiens
?
Other publictions for EC 1.1.1.172
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286113
Suda
Subcellular localization and t ...
Rattus sp.
Biochem. Biophys. Res. Commun.
77
586-591
1977
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1
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1
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6
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1
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1
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1
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1
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6
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286112
Suda
Purification and properties of ...
Homo sapiens
Arch. Biochem. Biophys.
176
610-620
1976
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-
-
-
-
-
4
2
-
-
1
1
-
2
-
-
1
-
-
2
-
1
1
-
-
-
-
-
-
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-
1
-
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-
-
-
1
-
-
-
-
4
-
2
-
-
1
1
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-
-
1
-
2
-
1
1
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