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show all sequences of 1.1.1.170

Identification of two novel mutations in the murine Nsdhl sterol dehydrogenase gene and development of a functional complementation assay in yeast

Lucas, M.E.; Ma, Q.; Cunningham, D.; Peters, J.; Cattanach, B.; Bard, M.; Elmore, B.K.; Herman, G.E.; Mol. Genet. Metab. 80, 227-233 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
complementation of erg26 deficient Saccharomyces cerevisiae strain SDG200
Mus musculus
Engineering
Amino acid exchange
Commentary
Organism
A94T
naturally occurring missense mutant, mutant is not able to complement erg26 deficiency in Saccharomyces cerevisiae strain SDG200
Mus musculus
G280A
loss of a HhaI restriction site
Mus musculus
V53D
naturally occurring missense mutant, mutant is not able to complement erg26 deficiency in Saccharomyces cerevisiae strain SDG200
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
involved in the removal of C-4 methyl groups in postsqualene cholesterol synthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
involved in the removal of C-4 methyl groups in postsqualene cholesterol synthesis
656813
Mus musculus
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
complementation of erg26 deficient Saccharomyces cerevisiae strain SDG200
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A94T
naturally occurring missense mutant, mutant is not able to complement erg26 deficiency in Saccharomyces cerevisiae strain SDG200
Mus musculus
G280A
loss of a HhaI restriction site
Mus musculus
V53D
naturally occurring missense mutant, mutant is not able to complement erg26 deficiency in Saccharomyces cerevisiae strain SDG200
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
involved in the removal of C-4 methyl groups in postsqualene cholesterol synthesis
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
involved in the removal of C-4 methyl groups in postsqualene cholesterol synthesis
656813
Mus musculus
?
-
-
-
-
Other publictions for EC 1.1.1.170
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
656813
Lucas
Identification of two novel mu ...
Mus musculus
Mol. Genet. Metab.
80
227-233
2003
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1
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3
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5
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1
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3
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1
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1
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722377
Caldas
NSDHL, an enzyme involved in c ...
Homo sapiens
Hum. Mol. Genet.
12
2981-91
2003
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2
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3
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2
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1
1
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723668
Gachotte
Characterization of the Saccha ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
95
13794-9
1998
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286111
Rahimtula
Investigation of the component ...
Rattus sp.
J. Biol. Chem.
247
9-15
1972
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4
1
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2
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1
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1
1
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1
1
1
2
-
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1
1
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1
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1
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4
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1
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2
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1
1
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1
1
1
2
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1
1
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