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Literature summary for 1.1.1.17 extracted from

  • Lopez, M.G.; Irla, M.; Brito, L.F.; Wendisch, V.F.
    Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus (2019), Front. Microbiol., 10, 1725 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.17

Cloned(Commentary)

Cloned (Comment) Organism
gene mtlD, transcriptional organization of the gene cluster, quantitative RT-PCR enzyme expression analysis Bacillus methanolicus

Protein Variants

Protein Variants Comment Organism
additional information functional complementation of arabitol dehydrogenase activity of gene MtlD in Corynebacterium glutamicum DELTAmtlD deletion mutant by gene atlD of Bacillus methanolicus strain MG3 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabitol 1-phosphate + NAD+ Bacillus methanolicus
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NAD+ Bacillus methanolicus ATCC 53907
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NAD+ Bacillus methanolicus MGA3
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+ Bacillus methanolicus
-
 D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+ Corynebacterium glutamicum
-
 D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+ Bacillus methanolicus ATCC 53907
-
 D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+ Bacillus methanolicus MGA3
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus I3E3X0
-
-
Bacillus methanolicus ATCC 53907 I3E3X0
-
-
Bacillus methanolicus MGA3 I3E3X0
-
-
Corynebacterium glutamicum A0A0U4Y4V3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information growth of Bacillus methanolicus strain MGA3 on arabitol as single carbon source and as co-substrate to mannitol. Although arabitol supports growth of strain MGA3 as a sole source of carbon and energy, the strain grows at a lower growth rate than with its preferred sugar alcohol substrate mannitol. Mannitol is utilized faster than arabitol and co-consumption of both sugar alcohols is observed, overview Bacillus methanolicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 arabitol phosphate dehydrogenase activity in mannitol grown cells of strains MG3, pH 7.2, 30°C Bacillus methanolicus
0.05
-
 arabitol phosphate dehydrogenase activity in arabitol grown cells of strains MG3, pH 7.2, 30°C Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NAD+
-
 Corynebacterium glutamicum D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus ATCC 53907 D-xylulose 5-phosphate + NADH + H+
-
 r
D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus MGA3 D-xylulose 5-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+
-
 Corynebacterium glutamicum D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus ATCC 53907 D-fructose 6-phosphate + NADH + H+
-
 r
D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus MGA3 D-fructose 6-phosphate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
BMMGA3_RS01080 locus name Bacillus methanolicus
More cf. EC 1.1.1.301 Bacillus methanolicus
MtlD
-
 Bacillus methanolicus
MtlD
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus methanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Bacillus methanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus methanolicus
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Bacillus methanolicus
NADH
-
 Corynebacterium glutamicum

Expression

Organism Comment Expression
Bacillus methanolicus in Bacillus methanolicus, RNA levels for atlD are higher while lower for mtlD during growth with both carbon sources as compared to growth with mannitol alone down
Corynebacterium glutamicum in Corynebacterium glutamicum, arabitol induces expression of genes rbtT, mtlD, sixA, xylB and atlR, and glucose represses expression of mtlD down
Bacillus methanolicus differential gene expression analysis of Bacillus methanolicus strain MGA3 cells grown on arabitol as compared to mannitol via transcriptome sequencing (RNA-seq), quantitative RT-PCR expression analysis, the enzyme can be induced by arabitol additional information

General Information

General Information Comment Organism
malfunction the Corynebacterium glutamicum arabitol-negative DELTAmtlD deletion mutant can be complemented by heterologous expression of Bacillus methanolicus strain MGA3 operon atlABCD or gene atlD Corynebacterium glutamicum
metabolism the enzyme is involved in the arabitol catabolism and the pentose phosphate pathway, model of the pathways for arabitol catabolism in bacteria, overview Bacillus methanolicus
physiological function Corynebacterium glutamicum is a natural D-arabitol utilizer that requires arabitol-utilizing mannitol-1-phosphate 5-dehydrogenase MtlD for arabitol catabolism Corynebacterium glutamicum