Literature summary for 1.1.1.17 extracted from

Kiser, R.C.; Niehaus, W.G.
Purification and kinetic characterization of mannitol-1-phosphate dehydrogenase from Aspergillus niger (1981), Arch. Biochem. Biophys., 211, 613-621.

Search for more literature for 1.1.1.17

Inhibitors

Inhibitors	Comment	Organism	Structure
adenosine diphosphoribose	competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate	Aspergillus niger
D-fructose 6-phosphate	competitive inhibition with respect to D-mannitol 1-phosphate	Aspergillus niger
D-glucose 6-phosphate	competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH	Aspergillus niger
D-mannitol 1-phosphate	competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH	Aspergillus niger
NAD+	competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate	Aspergillus niger
NADH	competitive inhibition with respect to NAD+	Aspergillus niger

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	NADH	cosubstrate: D-fructose 6-phosphate	Aspergillus niger
0.038	-	D-mannitol 1-phosphate	cosubstrate: NAD+	Aspergillus niger
0.083	-	NAD+	cosubstrate: D-mannnitol 1-phosphate	Aspergillus niger
0.54	-	D-fructose 6-phosphate	cosubstrate: NADH	Aspergillus niger

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Aspergillus niger	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22000	-	2 * 22000, SDS-PAGE	Aspergillus niger
40000	-	gel filtration	Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-mannitol 1-phosphate + NAD+	Aspergillus niger	highly specific for both substrates	D-fructose 6-phosphate + NADH + H+	-	r
D-mannitol 1-phosphate + NAD+	Aspergillus niger	random bi-bi kinetic with two dead-end complexes	D-fructose 6-phosphate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, chromatography techniques	Aspergillus niger

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Aspergillus niger	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
150	-	-	Aspergillus niger

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-mannitol 1-phosphate + acetylpyridine adenine dinucleotide	50% of the activity compared to NAD+	Aspergillus niger	D-fructose 6-phosphate + ?	-	?
D-mannitol 1-phosphate + NAD+	highly specific for both substrates	Aspergillus niger	D-fructose 6-phosphate + NADH + H+	-	r
D-mannitol 1-phosphate + NAD+	random bi-bi kinetic with two dead-end complexes	Aspergillus niger	D-fructose 6-phosphate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 22000, SDS-PAGE	Aspergillus niger

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10	more than 95% of its original activity	Aspergillus niger

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	strictly specific	Aspergillus niger
NADH	-	Aspergillus niger

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Release 2023.1 (January 2023)