BRENDA - Enzyme Database show
show all sequences of 1.1.1.168

Purification and characterization of oxopantoyl lactone reductase from higher plants: role in pantothenate synthesis

Julliard, J.H.; Bot. Acta 107, 191-200 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-keto-4-hydroxybutyrolactone
-
Spinacia oleracea
oxopyrrolidines
-
Spinacia oleracea
Reactive Red 3A
-
Spinacia oleracea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
isatin
-
Spinacia oleracea
0.012
-
bornanedione
-
Spinacia oleracea
0.015
-
8-bromobornanedione
-
Spinacia oleracea
0.026
-
oxopantoyl lactone
-
Spinacia oleracea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
36000
gel filtration, SDS-PAGE
Spinacia oleracea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
spinach
-
Purification (Commentary)
Commentary
Organism
-
Spinacia oleracea
Storage Stability
Storage Stability
Organism
4C, 1 year, 50% glycerol, 10% loss of activity
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydropantoyl lactone + NADPH
-
347731
Spinacia oleracea
(R)-pantoyl lactone + NADP+
-
-
-
ir
8-bromobornanedione + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
bornanedione + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
isatin + NADPH
-
347731
Spinacia oleracea
?
-
-
-
-
additional information
not: linear form of oxopantoate
347731
Spinacia oleracea
additional information
-
-
-
-
oxopantoyl lactone + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1* 36000, SDS-PAGE
Spinacia oleracea
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
0.35 M anhydrous sodium sulfate, complete inactivation
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Spinacia oleracea
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
strictly dependent on
Spinacia oleracea
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0025
-
Reactive Red 3A
-
Spinacia oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
strictly dependent on
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-keto-4-hydroxybutyrolactone
-
Spinacia oleracea
oxopyrrolidines
-
Spinacia oleracea
Reactive Red 3A
-
Spinacia oleracea
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0025
-
Reactive Red 3A
-
Spinacia oleracea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
isatin
-
Spinacia oleracea
0.012
-
bornanedione
-
Spinacia oleracea
0.015
-
8-bromobornanedione
-
Spinacia oleracea
0.026
-
oxopantoyl lactone
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
36000
gel filtration, SDS-PAGE
Spinacia oleracea
Purification (Commentary) (protein specific)
Commentary
Organism
-
Spinacia oleracea
Storage Stability (protein specific)
Storage Stability
Organism
4C, 1 year, 50% glycerol, 10% loss of activity
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydropantoyl lactone + NADPH
-
347731
Spinacia oleracea
(R)-pantoyl lactone + NADP+
-
-
-
ir
8-bromobornanedione + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
bornanedione + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
isatin + NADPH
-
347731
Spinacia oleracea
?
-
-
-
-
additional information
not: linear form of oxopantoate
347731
Spinacia oleracea
additional information
-
-
-
-
oxopantoyl lactone + NADPH
-
347731
Spinacia oleracea
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1* 36000, SDS-PAGE
Spinacia oleracea
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
0.35 M anhydrous sodium sulfate, complete inactivation
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Spinacia oleracea
Other publictions for EC 1.1.1.168
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
347731
Julliard
-
Purification and characterizat ...
Spinacia oleracea
Bot. Acta
107
191-200
1994
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
-
-
1
6
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
3
1
4
-
-
1
-
-
-
-
1
-
-
-
1
6
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
347730
Kataoka
Enzymes involved in the NADPH ...
Candida parapsilosis, Mucor ambiguus, Mucor ambiguus AKU 3006, Saccharomyces cerevisiae
Biosci. Biotechnol. Biochem.
56
820-821
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347723
Hata
Ketopantoyl-lactone reductase ...
Saccharomyces cerevisiae
FEMS Microbiol. Lett.
58
87-90
1989
-
-
-
-
-
-
3
16
-
-
-
1
-
2
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
16
-
-
-
1
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347724
Hata
Ketopantoyl-lactone reductase ...
Candida parapsilosis
Biochim. Biophys. Acta
990
175-181
1989
-
-
-
1
-
-
11
4
-
-
2
2
-
5
-
-
1
-
-
-
2
-
12
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
11
-
4
-
-
2
2
-
-
-
1
-
-
2
-
12
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
347726
Wilken
Ketopantoyl lactone reductases ...
Saccharomyces cerevisiae
Methods Enzymol.
62
209-215
1979
-
-
-
-
-
1
5
-
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
1
-
-
-
1
-
-
1
1
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347725
Wilken
Stereospecificity of pantoyl l ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
189
251-255
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286105
Wilken
Ketopantoic acid and ketopanto ...
Saccharomyces cerevisiae
J. Biol. Chem.
250
2311-2314
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
4
-
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-
1
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-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347727
King
Ketopantoyl lactone and ketopa ...
Saccharomyces cerevisiae
J. Biol. Chem.
249
4689-4695
1974
-
-
-
-
-
-
-
4
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286104
King
-
Separation and preliminary stu ...
Saccharomyces cerevisiae
J. Biol. Chem.
247
4096-4098
1972
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
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-
-
-
1
1
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-