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show all sequences of 1.1.1.168

Ketopantoyl-lactone reductase from Candida parapsilosis: purification and characterization as a conjugated polyketone reductase

Hata, H.; Shimizu, S.; Hattori, S.; Yamada, H.; Biochim. Biophys. Acta 990, 175-181 (1989)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Candida parapsilosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,4-Dihydroxy-3-cyclobutene-1,2-dione
uncompetitive to isatin
Candida parapsilosis
Al3+
-
Candida parapsilosis
Cd2+
-
Candida parapsilosis
Cu2+
-
Candida parapsilosis
Hg2+
-
Candida parapsilosis
iodoacetate
-
Candida parapsilosis
ketopantoyl lactone
competitive to isatin
Candida parapsilosis
p-chloromercuribenzoate
-
Candida parapsilosis
Polyketones
cyclohexenediol-1,2,3,4-tetraone; e.g. parabanic acid
Candida parapsilosis
quercetin
-
Candida parapsilosis
Zn2+
-
Candida parapsilosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
isatin
-
Candida parapsilosis
0.016
-
5-methylisatin
-
Candida parapsilosis
0.085
-
1-methylisatin
-
Candida parapsilosis
0.333
-
ketopantoyl lactone
-
Candida parapsilosis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
gel filtration
Candida parapsilosis
41600
-
1 * 41600, SDS-PAGE
Candida parapsilosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydropantoyl lactone + NADPH
Candida parapsilosis
-
(R)-pantoyl lactone + NADP+
-
Candida parapsilosis
ir
2-dehydropantoyl lactone + NADPH
Candida parapsilosis IFO 0708
-
(R)-pantoyl lactone + NADP+
-
Candida parapsilosis IFO 0708
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida parapsilosis
-
IFO 0708
-
Purification (Commentary)
Commentary
Organism
IFO 0708
Candida parapsilosis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
173
-
ketopantoyl lactone
Candida parapsilosis
251
-
isatin
Candida parapsilosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-methylisatin + NADPH
-
347724
Candida parapsilosis
?
-
-
-
-
2-dehydropantoyl lactone + NADPH
-
347724
Candida parapsilosis
(R)-pantoyl lactone + NADP+
-
347724
Candida parapsilosis
ir
2-dehydropantoyl lactone + NADPH
-
347724
Candida parapsilosis IFO 0708
(R)-pantoyl lactone + NADP+
-
347724
Candida parapsilosis IFO 0708
ir
5-methylisatin + NADPH
-
347724
Candida parapsilosis
?
-
-
-
-
camphorquinone + NADPH
-
347724
Candida parapsilosis
?
-
-
-
?
camphorquinone + NADPH
-
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
isatin + NADPH
and its derivatives
347724
Candida parapsilosis
?
-
-
-
?
isatin + NADPH
and its derivatives
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
additional information
carbonyl reductase specifically catalyzing the reduction of conjugated polyketones
347724
Candida parapsilosis
additional information
-
-
-
-
additional information
carbonyl reductase specifically catalyzing the reduction of conjugated polyketones
347724
Candida parapsilosis IFO 0708
additional information
-
-
-
-
naphthoquinone + NADPH
-
347724
Candida parapsilosis
?
-
-
-
?
naphthoquinone + NADPH
-
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 41600, SDS-PAGE
Candida parapsilosis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Candida parapsilosis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
pH 7.0, 10 min, 58% loss of activity
Candida parapsilosis
60
-
pH 7.0, 10 min, 77% loss of activity
Candida parapsilosis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
30C, 30 min, 33% loss of activity
Candida parapsilosis
6
10
-
Candida parapsilosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Candida parapsilosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,4-Dihydroxy-3-cyclobutene-1,2-dione
uncompetitive to isatin
Candida parapsilosis
Al3+
-
Candida parapsilosis
Cd2+
-
Candida parapsilosis
Cu2+
-
Candida parapsilosis
Hg2+
-
Candida parapsilosis
iodoacetate
-
Candida parapsilosis
ketopantoyl lactone
competitive to isatin
Candida parapsilosis
p-chloromercuribenzoate
-
Candida parapsilosis
Polyketones
cyclohexenediol-1,2,3,4-tetraone; e.g. parabanic acid
Candida parapsilosis
quercetin
-
Candida parapsilosis
Zn2+
-
Candida parapsilosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
isatin
-
Candida parapsilosis
0.016
-
5-methylisatin
-
Candida parapsilosis
0.085
-
1-methylisatin
-
Candida parapsilosis
0.333
-
ketopantoyl lactone
-
Candida parapsilosis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
gel filtration
Candida parapsilosis
41600
-
1 * 41600, SDS-PAGE
Candida parapsilosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydropantoyl lactone + NADPH
Candida parapsilosis
-
(R)-pantoyl lactone + NADP+
-
Candida parapsilosis
ir
2-dehydropantoyl lactone + NADPH
Candida parapsilosis IFO 0708
-
(R)-pantoyl lactone + NADP+
-
Candida parapsilosis IFO 0708
ir
Purification (Commentary) (protein specific)
Commentary
Organism
IFO 0708
Candida parapsilosis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
173
-
ketopantoyl lactone
Candida parapsilosis
251
-
isatin
Candida parapsilosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-methylisatin + NADPH
-
347724
Candida parapsilosis
?
-
-
-
-
2-dehydropantoyl lactone + NADPH
-
347724
Candida parapsilosis
(R)-pantoyl lactone + NADP+
-
347724
Candida parapsilosis
ir
2-dehydropantoyl lactone + NADPH
-
347724
Candida parapsilosis IFO 0708
(R)-pantoyl lactone + NADP+
-
347724
Candida parapsilosis IFO 0708
ir
5-methylisatin + NADPH
-
347724
Candida parapsilosis
?
-
-
-
-
camphorquinone + NADPH
-
347724
Candida parapsilosis
?
-
-
-
?
camphorquinone + NADPH
-
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
isatin + NADPH
and its derivatives
347724
Candida parapsilosis
?
-
-
-
?
isatin + NADPH
and its derivatives
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
additional information
carbonyl reductase specifically catalyzing the reduction of conjugated polyketones
347724
Candida parapsilosis
additional information
-
-
-
-
additional information
carbonyl reductase specifically catalyzing the reduction of conjugated polyketones
347724
Candida parapsilosis IFO 0708
additional information
-
-
-
-
naphthoquinone + NADPH
-
347724
Candida parapsilosis
?
-
-
-
?
naphthoquinone + NADPH
-
347724
Candida parapsilosis IFO 0708
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 41600, SDS-PAGE
Candida parapsilosis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Candida parapsilosis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
pH 7.0, 10 min, 58% loss of activity
Candida parapsilosis
60
-
pH 7.0, 10 min, 77% loss of activity
Candida parapsilosis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
30C, 30 min, 33% loss of activity
Candida parapsilosis
6
10
-
Candida parapsilosis
Other publictions for EC 1.1.1.168
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
347731
Julliard
-
Purification and characterizat ...
Spinacia oleracea
Bot. Acta
107
191-200
1994
-
-
-
-
-
-
3
4
-
-
1
-
-
1
-
-
1
-
-
-
-
1
6
1
-
-
1
-
1
-
-
1
1
-
-
-
-
-
1
-
-
-
-
3
1
4
-
-
1
-
-
-
-
1
-
-
-
1
6
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
347730
Kataoka
Enzymes involved in the NADPH ...
Candida parapsilosis, Mucor ambiguus, Mucor ambiguus AKU 3006, Saccharomyces cerevisiae
Biosci. Biotechnol. Biochem.
56
820-821
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
347723
Hata
Ketopantoyl-lactone reductase ...
Saccharomyces cerevisiae
FEMS Microbiol. Lett.
58
87-90
1989
-
-
-
-
-
-
3
16
-
-
-
1
-
2
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
16
-
-
-
1
-
-
-
-
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347724
Hata
Ketopantoyl-lactone reductase ...
Candida parapsilosis
Biochim. Biophys. Acta
990
175-181
1989
-
-
-
1
-
-
11
4
-
-
2
2
-
5
-
-
1
-
-
-
2
-
12
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
11
-
4
-
-
2
2
-
-
-
1
-
-
2
-
12
1
1
-
2
-
-
-
2
-
-
-
-
-
-
-
347726
Wilken
Ketopantoyl lactone reductases ...
Saccharomyces cerevisiae
Methods Enzymol.
62
209-215
1979
-
-
-
-
-
1
5
-
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
1
-
-
-
1
-
-
1
1
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
347725
Wilken
Stereospecificity of pantoyl l ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
189
251-255
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286105
Wilken
Ketopantoic acid and ketopanto ...
Saccharomyces cerevisiae
J. Biol. Chem.
250
2311-2314
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347727
King
Ketopantoyl lactone and ketopa ...
Saccharomyces cerevisiae
J. Biol. Chem.
249
4689-4695
1974
-
-
-
-
-
-
-
4
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286104
King
-
Separation and preliminary stu ...
Saccharomyces cerevisiae
J. Biol. Chem.
247
4096-4098
1972
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-