BRENDA - Enzyme Database show
show all sequences of 1.1.1.166

A new nicotinamide-adenine dinucleotide-dependent hydroaromatic dehydrogenase of Lactobacillus plantarum and its role in formation of (-)t-3,t-4-dihydrocyclohexane-c-1carboxylate

Whiting, G.C.; Coggins, R.A.; Biochem. J. 141, 35-42 (1974)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
NAD+
with (-)-shikimate
Lactobacillus plantarum
0.34
-
NAD+
with (+-)t-3,t-4-dihydroxycyclohexanecarboxylate and (-)-dihydroshikimate
Lactobacillus plantarum
0.36
-
NAD+
with (-)t-3,t-4-dihydroxycyclohexanecarboxylate
Lactobacillus plantarum
0.45
-
NAD+
with (-)-quinate
Lactobacillus plantarum
0.52
-
(-)-Dihydroshikimate
-
Lactobacillus plantarum
0.74
-
(-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
Lactobacillus plantarum
0.75
-
(-)-shikimate
-
Lactobacillus plantarum
0.85
-
(-)-quinate
-
Lactobacillus plantarum
1.55
-
(+-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+
Lactobacillus plantarum
-
(1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
Lactobacillus plantarum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus plantarum
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Lactobacillus plantarum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Lactobacillus plantarum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
58.9
-
-
Lactobacillus plantarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate + NAD+
-
286100
Lactobacillus plantarum
(+-)t-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
286100
Lactobacillus plantarum
?
(-)-dihydroshikimate + NAD+
-
286100
Lactobacillus plantarum
? + NADH
-
286100
Lactobacillus plantarum
?
(-)-quinate + NAD+
-
286100
Lactobacillus plantarum
3-dehydroquinate + NADH + H+
-
286100
Lactobacillus plantarum
r
(-)-shikimate + NAD+
more rapidly oxidized than other acids
286100
Lactobacillus plantarum
(-)-3-dehydroshikimate + NADH + H+
-
286100
Lactobacillus plantarum
r
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+
-
286100
Lactobacillus plantarum
(1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
286100
Lactobacillus plantarum
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
reductive direction
Lactobacillus plantarum
10
10.1
oxidative direction
Lactobacillus plantarum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
active within, reductive direction
Lactobacillus plantarum
6.9
11.4
active within, oxidative direction
Lactobacillus plantarum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Lactobacillus plantarum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
can not be replaced by NADP+
Lactobacillus plantarum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
NAD+
with (-)-shikimate
Lactobacillus plantarum
0.34
-
NAD+
with (+-)t-3,t-4-dihydroxycyclohexanecarboxylate and (-)-dihydroshikimate
Lactobacillus plantarum
0.36
-
NAD+
with (-)t-3,t-4-dihydroxycyclohexanecarboxylate
Lactobacillus plantarum
0.45
-
NAD+
with (-)-quinate
Lactobacillus plantarum
0.52
-
(-)-Dihydroshikimate
-
Lactobacillus plantarum
0.74
-
(-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
Lactobacillus plantarum
0.75
-
(-)-shikimate
-
Lactobacillus plantarum
0.85
-
(-)-quinate
-
Lactobacillus plantarum
1.55
-
(+-)t-3,t-4-dihydroxycyclohexanecarboxylate
-
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+
Lactobacillus plantarum
-
(1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
Lactobacillus plantarum
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Lactobacillus plantarum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Lactobacillus plantarum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
58.9
-
-
Lactobacillus plantarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate + NAD+
-
286100
Lactobacillus plantarum
(+-)t-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
286100
Lactobacillus plantarum
?
(-)-dihydroshikimate + NAD+
-
286100
Lactobacillus plantarum
? + NADH
-
286100
Lactobacillus plantarum
?
(-)-quinate + NAD+
-
286100
Lactobacillus plantarum
3-dehydroquinate + NADH + H+
-
286100
Lactobacillus plantarum
r
(-)-shikimate + NAD+
more rapidly oxidized than other acids
286100
Lactobacillus plantarum
(-)-3-dehydroshikimate + NADH + H+
-
286100
Lactobacillus plantarum
r
(1S,3R,4S)-3,4-dihydroxycyclohexane-1-carboxylate + NAD+
-
286100
Lactobacillus plantarum
(1S,4S)-4-hydroxy-3-oxocyclohexane-1-carboxylate + NADH
-
286100
Lactobacillus plantarum
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
reductive direction
Lactobacillus plantarum
10
10.1
oxidative direction
Lactobacillus plantarum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
active within, reductive direction
Lactobacillus plantarum
6.9
11.4
active within, oxidative direction
Lactobacillus plantarum
Other publictions for EC 1.1.1.166
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286100
Whiting
A new nicotinamide-adenine din ...
Lactobacillus plantarum
Biochem. J.
141
35-42
1974
-
-
-
-
-
-
-
9
-
-
-
1
-
1
-
-
1
-
-
1
1
-
5
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
1
-
1
1
-
5
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-