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show all sequences of 1.1.1.165

Purification and characterization of 2-alkyne-1-ol dehydrogenase induced by 2-butyne-1,4-diol in Fusarium merismoides B11

Miyoshi, T.; Sato, H.; Harada, T.; Biochim. Biophys. Acta 358, 231-239 (1974)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
highly labile, even in the presence of DTT
Fusarium merismoides
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuCl2+
complete inhibition at 2 mM
Fusarium merismoides
HgCl2
complete inhibition at 2 mM
Fusarium merismoides
iodoacetic acid
complete inhibition at 2 mM
Fusarium merismoides
KCN
slight inhibition at 2 mM
Fusarium merismoides
NaN3
slight inhibition at 2 mM
Fusarium merismoides
Ni2+
84% inhibition at 2 mM
Fusarium merismoides
p-chloromercuribenzoate
complete inhibition at 2 mM
Fusarium merismoides
Zn2+
55% inhibition at 2 mM
Fusarium merismoides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.1
-
2-butyne-1,4-diol
-
Fusarium merismoides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-butyne-1,4-diol + NAD+
Fusarium merismoides
-
4-hydroxybut-2-ynal + NADH
-
Fusarium merismoides
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fusarium merismoides
-
strainB11
-
Fusarium merismoides strainB11
-
strainB11
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Fusarium merismoides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:2-butyne-1,4-diol-grown cell
highest induction of enzyme formation by use of 2-butyne-1,4-diol-containing growth media
Fusarium merismoides
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
751.1
-
-
Fusarium merismoides
Storage Stability
Storage Stability
Organism
5°C, 2 days
Fusarium merismoides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,4-butanetriol + NAD+
low activity
286099
Fusarium merismoides
? + NADH
-
286099
Fusarium merismoides
?
1,2,4-butanetriol + NAD+
low activity
286099
Fusarium merismoides strainB11
? + NADH
-
286099
Fusarium merismoides strainB11
?
1,2-butanediol + NAD+
low activity
286099
Fusarium merismoides
1-hydroxybutan-2-one + NADH
-
286099
Fusarium merismoides
?
1,2-butanediol + NAD+
low activity
286099
Fusarium merismoides strainB11
1-hydroxybutan-2-one + NADH
-
286099
Fusarium merismoides strainB11
?
1,2-propanediol + NAD+
-
286099
Fusarium merismoides
1-hydroxy-2-propanal + NADH
-
286099
Fusarium merismoides
?
1,2-propanediol + NAD+
-
286099
Fusarium merismoides strainB11
1-hydroxy-2-propanal + NADH
-
286099
Fusarium merismoides strainB11
?
1,4-butanediol + NAD+
-
286099
Fusarium merismoides
1-hydroxy-4-butanal + NADH
-
286099
Fusarium merismoides
?
1,4-butanediol + NAD+
-
286099
Fusarium merismoides strainB11
1-hydroxy-4-butanal + NADH
-
286099
Fusarium merismoides strainB11
?
1,6-hexanediol + NAD+
low activity
286099
Fusarium merismoides
1-hydroxy-6-hexanal + NADH
-
286099
Fusarium merismoides
?
1-butene-3-ol + NAD+
low activity
286099
Fusarium merismoides
1-buten-3-one + NADH
-
286099
Fusarium merismoides
?
1-butyne-3-ol + NAD+
very low activity
286099
Fusarium merismoides
1-butyne-3-one + NADH
-
286099
Fusarium merismoides
?
2-butene-1-ol + NAD+
cis-trans mixture, very low activity
286099
Fusarium merismoides
2-butene-1-al + NADH
-
286099
Fusarium merismoides
?
2-butyne-1,4-diol + NAD+
-
286099
Fusarium merismoides
4-hydroxybut-2-ynal + NADH
-
286099
Fusarium merismoides
?
2-butyne-1-ol + NAD+
highest activity
286099
Fusarium merismoides
2-butyn-1-al + NADH
-
286099
Fusarium merismoides
?
2-propene-1-ol + NAD+
low activity
286099
Fusarium merismoides
2-propene-1-al + NADH
-
286099
Fusarium merismoides
?
2-propyne-1-ol + NAD+
-
286099
Fusarium merismoides
2-propyn-1-al + NADH
-
286099
Fusarium merismoides
?
3-butyne-1-ol + NAD+
very low activity
286099
Fusarium merismoides
3-butyn-1-al + NADH
-
286099
Fusarium merismoides
?
n-propanol + NAD+
low activity
286099
Fusarium merismoides
n-propanal + NADH
-
286099
Fusarium merismoides
?
n-propanol + NAD+
low activity
286099
Fusarium merismoides strainB11
n-propanal + NADH
-
286099
Fusarium merismoides strainB11
?
trans-2-butene-1,4-diol + NAD+
-
286099
Fusarium merismoides
? + NADH
-
286099
Fusarium merismoides
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable below, rapid decrease of activity above
Fusarium merismoides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Fusarium merismoides
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
8.5
stable between
Fusarium merismoides
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Fusarium merismoides
NADP+
one third of the activity compared to NAD+
Fusarium merismoides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Fusarium merismoides
NADP+
one third of the activity compared to NAD+
Fusarium merismoides
General Stability (protein specific)
General Stability
Organism
highly labile, even in the presence of DTT
Fusarium merismoides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuCl2+
complete inhibition at 2 mM
Fusarium merismoides
HgCl2
complete inhibition at 2 mM
Fusarium merismoides
iodoacetic acid
complete inhibition at 2 mM
Fusarium merismoides
KCN
slight inhibition at 2 mM
Fusarium merismoides
NaN3
slight inhibition at 2 mM
Fusarium merismoides
Ni2+
84% inhibition at 2 mM
Fusarium merismoides
p-chloromercuribenzoate
complete inhibition at 2 mM
Fusarium merismoides
Zn2+
55% inhibition at 2 mM
Fusarium merismoides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
9.1
-
2-butyne-1,4-diol
-
Fusarium merismoides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-butyne-1,4-diol + NAD+
Fusarium merismoides
-
4-hydroxybut-2-ynal + NADH
-
Fusarium merismoides
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Fusarium merismoides
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:2-butyne-1,4-diol-grown cell
highest induction of enzyme formation by use of 2-butyne-1,4-diol-containing growth media
Fusarium merismoides
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
751.1
-
-
Fusarium merismoides
Storage Stability (protein specific)
Storage Stability
Organism
5°C, 2 days
Fusarium merismoides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,4-butanetriol + NAD+
low activity
286099
Fusarium merismoides
? + NADH
-
286099
Fusarium merismoides
?
1,2,4-butanetriol + NAD+
low activity
286099
Fusarium merismoides strainB11
? + NADH
-
286099
Fusarium merismoides strainB11
?
1,2-butanediol + NAD+
low activity
286099
Fusarium merismoides
1-hydroxybutan-2-one + NADH
-
286099
Fusarium merismoides
?
1,2-butanediol + NAD+
low activity
286099
Fusarium merismoides strainB11
1-hydroxybutan-2-one + NADH
-
286099
Fusarium merismoides strainB11
?
1,2-propanediol + NAD+
-
286099
Fusarium merismoides
1-hydroxy-2-propanal + NADH
-
286099
Fusarium merismoides
?
1,2-propanediol + NAD+
-
286099
Fusarium merismoides strainB11
1-hydroxy-2-propanal + NADH
-
286099
Fusarium merismoides strainB11
?
1,4-butanediol + NAD+
-
286099
Fusarium merismoides
1-hydroxy-4-butanal + NADH
-
286099
Fusarium merismoides
?
1,4-butanediol + NAD+
-
286099
Fusarium merismoides strainB11
1-hydroxy-4-butanal + NADH
-
286099
Fusarium merismoides strainB11
?
1,6-hexanediol + NAD+
low activity
286099
Fusarium merismoides
1-hydroxy-6-hexanal + NADH
-
286099
Fusarium merismoides
?
1-butene-3-ol + NAD+
low activity
286099
Fusarium merismoides
1-buten-3-one + NADH
-
286099
Fusarium merismoides
?
1-butyne-3-ol + NAD+
very low activity
286099
Fusarium merismoides
1-butyne-3-one + NADH
-
286099
Fusarium merismoides
?
2-butene-1-ol + NAD+
cis-trans mixture, very low activity
286099
Fusarium merismoides
2-butene-1-al + NADH
-
286099
Fusarium merismoides
?
2-butyne-1,4-diol + NAD+
-
286099
Fusarium merismoides
4-hydroxybut-2-ynal + NADH
-
286099
Fusarium merismoides
?
2-butyne-1-ol + NAD+
highest activity
286099
Fusarium merismoides
2-butyn-1-al + NADH
-
286099
Fusarium merismoides
?
2-propene-1-ol + NAD+
low activity
286099
Fusarium merismoides
2-propene-1-al + NADH
-
286099
Fusarium merismoides
?
2-propyne-1-ol + NAD+
-
286099
Fusarium merismoides
2-propyn-1-al + NADH
-
286099
Fusarium merismoides
?
3-butyne-1-ol + NAD+
very low activity
286099
Fusarium merismoides
3-butyn-1-al + NADH
-
286099
Fusarium merismoides
?
n-propanol + NAD+
low activity
286099
Fusarium merismoides
n-propanal + NADH
-
286099
Fusarium merismoides
?
n-propanol + NAD+
low activity
286099
Fusarium merismoides strainB11
n-propanal + NADH
-
286099
Fusarium merismoides strainB11
?
trans-2-butene-1,4-diol + NAD+
-
286099
Fusarium merismoides
? + NADH
-
286099
Fusarium merismoides
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable below, rapid decrease of activity above
Fusarium merismoides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Fusarium merismoides
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
8.5
stable between
Fusarium merismoides
Other publictions for EC 1.1.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286099
Miyoshi
-
Purification and characterizat ...
Fusarium merismoides, Fusarium merismoides strainB11
Biochim. Biophys. Acta
358
231-239
1974
-
-
-
-
-
1
8
1
-
-
-
1
-
2
-
-
1
-
-
1
1
1
20
-
-
-
1
-
1
-
1
2
-
-
-
-
-
-
2
-
-
1
-
8
-
1
-
-
-
1
-
-
-
1
-
1
1
1
20
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-