BRENDA - Enzyme Database show
show all sequences of 1.1.1.164

Alcohol dehydrogenase in Acinobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism

Singer, M.E.; Finnerty, W.R.; J. Bacteriol. 164, 1017-1024 (1985)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0016
-
hexadecanol
in crude extract of hexadecane-induced cells
Acinetobacter sp.
0.0028
-
hexadecanol
in crude extract of hexadecanol-induced cells
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp.
?
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp. HO1-N
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter sp.
-
strain HO1-N
-
Acinetobacter sp. HO1-N
-
strain HO1-N
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0008
-
growth substrate of cells ethanol, assay substrate n-hexadecanol
Acinetobacter sp.
0.0011
-
growth substrate of cells palmitic acid, assay substrate n-hexadecanol
Acinetobacter sp.
0.0046
-
growth substrate of cells hexadecanol, assay substrate n-hexadecanol
Acinetobacter sp.
0.0059
-
growth substrate of cells hexadecane, assay substrate n-hexadecanol
Acinetobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hexadecanal + NADH
-
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp.
hexadecanal + NADH
-
286098
Acinetobacter sp.
?
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp. HO1-N
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preference for
Acinetobacter sp.
NADP+
low activity
Acinetobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preference for
Acinetobacter sp.
NADP+
low activity
Acinetobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0016
-
hexadecanol
in crude extract of hexadecane-induced cells
Acinetobacter sp.
0.0028
-
hexadecanol
in crude extract of hexadecanol-induced cells
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hexadecanal + NADH
Acinetobacter sp.
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp.
?
hexadecanal + NADH
Acinetobacter sp. HO1-N
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
hexadecanol + NAD+
-
Acinetobacter sp. HO1-N
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0008
-
growth substrate of cells ethanol, assay substrate n-hexadecanol
Acinetobacter sp.
0.0011
-
growth substrate of cells palmitic acid, assay substrate n-hexadecanol
Acinetobacter sp.
0.0046
-
growth substrate of cells hexadecanol, assay substrate n-hexadecanol
Acinetobacter sp.
0.0059
-
growth substrate of cells hexadecane, assay substrate n-hexadecanol
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hexadecanal + NADH
-
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp.
hexadecanol + NAD+
-
286098
Acinetobacter sp.
?
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
hexadecanal + NADH
inducible 72fold by growth on hexadecanol, also inducible by ethanol, palmitate or hexadecane
286098
Acinetobacter sp. HO1-N
hexadecanol + NAD+
-
286098
Acinetobacter sp. HO1-N
?
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp.
hexadecanal + NADH
-
286098
Acinetobacter sp.
?
hexadecanol + NAD+
enzyme is important for alkane dissimilation
286098
Acinetobacter sp. HO1-N
hexadecanal + NADH
-
286098
Acinetobacter sp. HO1-N
?
Other publictions for EC 1.1.1.164
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
667237
Duron-Castellanos
Detection of NAD+dependent alc ...
Mucor circinelloides, Mucor circinelloides YR-1
Appl. Biochem. Biotechnol.
121-124
279-288
2005
-
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-
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1
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5
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1
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1
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2
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1
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-
286097
Finnerty
Primary alcohol dehydrogenases ...
Acinetobacter sp., Acinetobacter sp. HO1-N
Methods Enzymol.
188
14-18
1990
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1
1
2
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2
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3
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1
4
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1
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1
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2
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1
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1
4
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286098
Singer
Alcohol dehydrogenase in Acino ...
Acinetobacter sp., Acinetobacter sp. HO1-N
J. Bacteriol.
164
1017-1024
1985
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2
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2
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5
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6
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286095
Kolattukudy
Reduction of fatty acids to al ...
Euglena gracilis, Euglena gracilis Z
Biochemistry
9
1095-1102
1970
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21
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4
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4
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286096
Stoffel
Metabolism of sphingosine base ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
351
875-883
1970
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1
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