BRENDA - Enzyme Database show
show all sequences of 1.1.1.163

Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp. strain N.C.I.M.B. 9872 and biotransformations effected by Eschericha coli-expressed cyclopentanone 1,2-monooxygenase

Iwaki, H.; Hasegawa, Y.; Wang, S.; Kayser, M.M.; Lau, P.C.K.; Appl. Environ. Microbiol. 68, 5671-5684 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Comamonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-cyclohexandiol + NAD+
20-30% of the activity with cyclopentanol
439022
Comamonas sp.
4-hydroxy-cyclohexanone + NADH
-
-
-
?
2-methylcyclohexanol + NAD+
20-30% of the activity with cyclopentanol
439022
Comamonas sp.
2-methyl-cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
60% of the activity with cyclopentanol
439022
Comamonas sp.
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
-
439022
Comamonas sp.
cyclopentanone + NADH
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Comamonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-cyclohexandiol + NAD+
20-30% of the activity with cyclopentanol
439022
Comamonas sp.
4-hydroxy-cyclohexanone + NADH
-
-
-
?
2-methylcyclohexanol + NAD+
20-30% of the activity with cyclopentanol
439022
Comamonas sp.
2-methyl-cyclohexanone + NADH
-
-
-
?
cyclohexanol + NAD+
60% of the activity with cyclopentanol
439022
Comamonas sp.
cyclohexanone + NADH
-
-
-
?
cyclopentanol + NAD+
-
439022
Comamonas sp.
cyclopentanone + NADH
-
-
-
?
Other publictions for EC 1.1.1.163
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439022
Iwaki
Cloning and characterization o ...
Comamonas sp.
Appl. Environ. Microbiol.
68
5671-5684
2002
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4
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1
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4
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286094
Griffin
The metabolism of cyclopentano ...
Pseudomonas sp., Pseudomonas sp. N.C.I.B. 9872
Biochem. J.
129
595-603
1972
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2
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2
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1
1
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2
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1
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1
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1
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2
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1
1
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2
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1
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