BRENDA - Enzyme Database show
show all sequences of 1.1.1.162

D-erythrulose reductase can also reduce diacetyl: further purification and characterization of D-erythrulose reductase from chicken liver

Maeda, M.; Hosomi, S.; Mizoguchi, T.; Nishihara, T.; J. Biochem. 123, 602-606 (1998)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
Phenanthrenequinone
-
Gallus gallus
0.11
-
D-erythrulose
-
Gallus gallus
0.19
-
3,4-hexanedione
-
Gallus gallus
0.24
-
2,3-Pentanedione
-
Gallus gallus
0.26
-
2,3-heptanedione
-
Gallus gallus
0.39
-
diacetyl
-
Gallus gallus
0.67
-
1,2-Cyclohexanedione
-
Gallus gallus
0.68
-
(1S)-(+)-Camphorquinone
-
Gallus gallus
0.72
-
isatin
-
Gallus gallus
1.1
-
ethyl pyruvate
-
Gallus gallus
1.1
-
Methyl pyruvate
-
Gallus gallus
13
-
acetoin
-
Gallus gallus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacetyl + NAD(P)H
Gallus gallus
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
acetoin + NADP+
-
Gallus gallus
ir
diacetyl + NAD(P)H
Gallus gallus
detoxification of food derived dicarbonyls
acetoin + NADP+
-
Gallus gallus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gallus gallus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Gallus gallus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Gallus gallus
-
brain
-
Gallus gallus
-
heart
-
Gallus gallus
-
intestine
-
Gallus gallus
-
kidney
-
Gallus gallus
-
liver
-
Gallus gallus
-
additional information
minimal activity also in lens, spleen, lung and pancreas
Gallus gallus
-
stomach
-
Gallus gallus
-
testis
-
Gallus gallus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.021
-
acetoin
Gallus gallus
64
-
(1S)-(+)-camphorquinone
Gallus gallus
77
-
methyl pyruvate
Gallus gallus
83
-
phenanthrenequione
Gallus gallus
120
-
1,2-cyclohexanedione; 2,3-pentanedione; ethyl pyruvate
Gallus gallus
130
-
diacetyl
Gallus gallus
180
-
2,3-heptanedione
Gallus gallus
200
-
3,4-hexanedione
Gallus gallus
250
-
isatin
Gallus gallus
320
-
purified enzyme, D-erythrulose
Gallus gallus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1S)-(+)-camphorquinone + NADPH
stereospecific
286091
Gallus gallus
?
-
-
-
?
1,2-cyclohexanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
2,3-heptanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
2,3-pentanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
3,4-hexanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
acetoin + NADPH + H+
only slight reversibility, low activity
286091
Gallus gallus
2,3-butanediol + NADP+
-
286091
Gallus gallus
r
D-erythrulose + NAD(P)H
-
286091
Gallus gallus
D-threitol + NAD(P)+
-
-
-
r
diacetyl + NAD(P)H
utilizes mainly vicinal alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
utilizes mainly vicinal alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
specific for alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
specific for alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
detoxification of food derived dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
ethyl pyruvate + NADPH
-
286091
Gallus gallus
ethyllactate + NADP+
-
-
-
?
isatin + NADPH
s-cis configuration of the neighbouring carbonyls is important for activity
286091
Gallus gallus
?
-
-
-
?
methyl pyruvate + NADPH
-
286091
Gallus gallus
methyllactate + NADP+
-
-
-
?
Phenanthrenequinone + NADPH
s-cis configuration of the neighbouring carbonyls is important for activity
286091
Gallus gallus
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
6
diacetyl + NADPH
Gallus gallus
6
6.8
diacetyl + NADH
Gallus gallus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
oxidation of acetoin to 2,3-butanediol
Gallus gallus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Gallus gallus
NADP+
-
Gallus gallus
NADPH
-
Gallus gallus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Gallus gallus
NADP+
-
Gallus gallus
NADPH
-
Gallus gallus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
Phenanthrenequinone
-
Gallus gallus
0.11
-
D-erythrulose
-
Gallus gallus
0.19
-
3,4-hexanedione
-
Gallus gallus
0.24
-
2,3-Pentanedione
-
Gallus gallus
0.26
-
2,3-heptanedione
-
Gallus gallus
0.39
-
diacetyl
-
Gallus gallus
0.67
-
1,2-Cyclohexanedione
-
Gallus gallus
0.68
-
(1S)-(+)-Camphorquinone
-
Gallus gallus
0.72
-
isatin
-
Gallus gallus
1.1
-
ethyl pyruvate
-
Gallus gallus
1.1
-
Methyl pyruvate
-
Gallus gallus
13
-
acetoin
-
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
diacetyl + NAD(P)H
Gallus gallus
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
acetoin + NADP+
-
Gallus gallus
ir
diacetyl + NAD(P)H
Gallus gallus
detoxification of food derived dicarbonyls
acetoin + NADP+
-
Gallus gallus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gallus gallus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Gallus gallus
-
brain
-
Gallus gallus
-
heart
-
Gallus gallus
-
intestine
-
Gallus gallus
-
kidney
-
Gallus gallus
-
liver
-
Gallus gallus
-
additional information
minimal activity also in lens, spleen, lung and pancreas
Gallus gallus
-
stomach
-
Gallus gallus
-
testis
-
Gallus gallus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.021
-
acetoin
Gallus gallus
64
-
(1S)-(+)-camphorquinone
Gallus gallus
77
-
methyl pyruvate
Gallus gallus
83
-
phenanthrenequione
Gallus gallus
120
-
1,2-cyclohexanedione; 2,3-pentanedione; ethyl pyruvate
Gallus gallus
130
-
diacetyl
Gallus gallus
180
-
2,3-heptanedione
Gallus gallus
200
-
3,4-hexanedione
Gallus gallus
250
-
isatin
Gallus gallus
320
-
purified enzyme, D-erythrulose
Gallus gallus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1S)-(+)-camphorquinone + NADPH
stereospecific
286091
Gallus gallus
?
-
-
-
?
1,2-cyclohexanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
2,3-heptanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
2,3-pentanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
3,4-hexanedione + NADPH
-
286091
Gallus gallus
?
-
-
-
?
acetoin + NADPH + H+
only slight reversibility, low activity
286091
Gallus gallus
2,3-butanediol + NADP+
-
286091
Gallus gallus
r
D-erythrulose + NAD(P)H
-
286091
Gallus gallus
D-threitol + NAD(P)+
-
-
-
r
diacetyl + NAD(P)H
utilizes mainly vicinal alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
utilizes mainly vicinal alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
reaction is also performed by acetoin:NAD+ oxidoreductase, EC 1.1.1.5
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
specific for alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
?
diacetyl + NAD(P)H
specific for alpha-dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
diacetyl + NAD(P)H
detoxification of food derived dicarbonyls
286091
Gallus gallus
acetoin + NADP+
-
286091
Gallus gallus
ir
ethyl pyruvate + NADPH
-
286091
Gallus gallus
ethyllactate + NADP+
-
-
-
?
isatin + NADPH
s-cis configuration of the neighbouring carbonyls is important for activity
286091
Gallus gallus
?
-
-
-
?
methyl pyruvate + NADPH
-
286091
Gallus gallus
methyllactate + NADP+
-
-
-
?
Phenanthrenequinone + NADPH
s-cis configuration of the neighbouring carbonyls is important for activity
286091
Gallus gallus
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
6
diacetyl + NADPH
Gallus gallus
6
6.8
diacetyl + NADH
Gallus gallus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
oxidation of acetoin to 2,3-butanediol
Gallus gallus
Other publictions for EC 1.1.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286093
Maeda
Cloning and sequence analysis ...
Gallus gallus
Protein Eng.
15
611-617
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286091
Maeda
D-erythrulose reductase can al ...
Gallus gallus
J. Biochem.
123
602-606
1998
-
-
-
-
-
-
-
12
-
-
-
2
-
3
-
-
1
-
-
10
10
-
18
-
-
-
-
-
2
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
12
-
-
-
2
-
-
-
1
-
10
10
-
18
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286092
Morii
Methods for enzymatic and colo ...
Bos taurus, Gallus gallus
Anal. Biochem.
151
188-191
1985
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
2
-
4
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
286086
Uehara
D-Erythrulose reductase from b ...
Bos taurus
Methods Enzymol.
89
232-237
1982
-
-
-
1
-
1
3
3
-
-
2
-
-
2
1
-
1
1
-
5
1
1
2
1
-
-
1
-
2
-
-
3
-
-
-
-
-
-
3
1
-
1
-
3
-
3
-
-
2
-
-
1
-
1
-
5
1
1
2
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
286088
Uehara
Studies on D-tetrose metabolis ...
Gallus gallus
J. Biochem.
87
47-55
1980
-
-
-
1
-
-
-
3
-
-
2
1
-
3
-
-
1
-
-
2
1
-
3
1
1
-
1
-
3
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
2
1
-
-
-
1
-
2
1
-
3
1
1
-
1
-
3
-
-
-
-
-
-
-
-
-
286090
Uehara
Effect of NADP+ and its analog ...
Bos taurus
J. Biochem.
85
1003-1008
1979
-
-
-
-
-
2
3
-
-
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286089
Uehara
Studies on D-tetrose metabolis ...
Bos taurus
J. Biochem.
78
519-526
1975
-
-
-
1
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
286087
Uehara
Studies on D-tetrose metabolis ...
Bos taurus
J. Biochem.
75
333-345
1974
-
-
-
-
-
1
-
2
-
-
2
-
-
2
-
-
1
-
-
2
1
-
2
1
-
-
3
-
2
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
-
2
-
-
-
-
1
-
2
1
-
2
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-