BRENDA - Enzyme Database show
show all sequences of 1.1.1.162

Studies on D-tetrose metabolism. IV. Purification and some properties of D-erythrulose reductase from beef liver

Uehara, K.; Tanimoto, T.; Sato, H.; J. Biochem. 75, 333-345 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0068
-
NADPH
-
Bos taurus
0.22
-
NADH
-
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
4 * 22000, SDS-PAGE
Bos taurus
90000
-
sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
123
-
-
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-erythrulose + NAD(P)H
highly specific for D-erythrulose
286087
Bos taurus
D-threitol + NAD(P)+
-
286087
Bos taurus
ir
D-erythrulose + NAD(P)H
reversibility could not be demonstrated
286087
Bos taurus
D-threitol + NAD(P)+
-
286087
Bos taurus
ir
Subunits
Subunits
Commentary
Organism
tetramer
4 * 22000, SDS-PAGE
Bos taurus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
cold inactivation
Bos taurus
-
-
23.5 h, 90% loss of activity, regains about 55-65% of its original activity after 60 min
Bos taurus
21
-
23.5 h, 20-25% loss of activity
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.85
-
D-erythrulose + NADH
Bos taurus
6.25
-
D-erythrulose + NADH
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
utilized more slowly than NADPH
Bos taurus
NADPH
-
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
utilized more slowly than NADPH
Bos taurus
NADPH
-
Bos taurus
General Stability (protein specific)
General Stability
Organism
NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0068
-
NADPH
-
Bos taurus
0.22
-
NADH
-
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
4 * 22000, SDS-PAGE
Bos taurus
90000
-
sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
123
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-erythrulose + NAD(P)H
highly specific for D-erythrulose
286087
Bos taurus
D-threitol + NAD(P)+
-
286087
Bos taurus
ir
D-erythrulose + NAD(P)H
reversibility could not be demonstrated
286087
Bos taurus
D-threitol + NAD(P)+
-
286087
Bos taurus
ir
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 22000, SDS-PAGE
Bos taurus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
cold inactivation
Bos taurus
-
-
23.5 h, 90% loss of activity, regains about 55-65% of its original activity after 60 min
Bos taurus
21
-
23.5 h, 20-25% loss of activity
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.85
-
D-erythrulose + NADH
Bos taurus
6.25
-
D-erythrulose + NADH
Bos taurus
Other publictions for EC 1.1.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286093
Maeda
Cloning and sequence analysis ...
Gallus gallus
Protein Eng.
15
611-617
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286091
Maeda
D-erythrulose reductase can al ...
Gallus gallus
J. Biochem.
123
602-606
1998
-
-
-
-
-
-
-
12
-
-
-
2
-
3
-
-
1
-
-
10
10
-
18
-
-
-
-
-
2
1
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
12
-
-
-
2
-
-
-
1
-
10
10
-
18
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286092
Morii
Methods for enzymatic and colo ...
Bos taurus, Gallus gallus
Anal. Biochem.
151
188-191
1985
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
2
-
4
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
286086
Uehara
D-Erythrulose reductase from b ...
Bos taurus
Methods Enzymol.
89
232-237
1982
-
-
-
1
-
1
3
3
-
-
2
-
-
2
1
-
1
1
-
5
1
1
2
1
-
-
1
-
2
-
-
3
-
-
-
-
-
-
3
1
-
1
-
3
-
3
-
-
2
-
-
1
-
1
-
5
1
1
2
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
286088
Uehara
Studies on D-tetrose metabolis ...
Gallus gallus
J. Biochem.
87
47-55
1980
-
-
-
1
-
-
-
3
-
-
2
1
-
3
-
-
1
-
-
2
1
-
3
1
1
-
1
-
3
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
2
1
-
-
-
1
-
2
1
-
3
1
1
-
1
-
3
-
-
-
-
-
-
-
-
-
286090
Uehara
Effect of NADP+ and its analog ...
Bos taurus
J. Biochem.
85
1003-1008
1979
-
-
-
-
-
2
3
-
-
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286089
Uehara
Studies on D-tetrose metabolis ...
Bos taurus
J. Biochem.
78
519-526
1975
-
-
-
1
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
286087
Uehara
Studies on D-tetrose metabolis ...
Bos taurus
J. Biochem.
75
333-345
1974
-
-
-
-
-
1
-
2
-
-
2
-
-
2
-
-
1
-
-
2
1
-
2
1
-
-
3
-
2
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
-
2
-
-
-
-
1
-
2
1
-
2
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-