BRENDA - Enzyme Database show
show all sequences of 1.1.1.159

Cloning and sequencing of the 7alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme

Yoshimoto, T.; Higashi, H.; Kanatani, A.; Lin, X.S.; Nagai, H.; Oyama, H.; Kurazono, K.; Tsuru, D.; J. Bacteriol. 173, 2173-2179 (1991)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene from strain HB101 cloned in plasmid pSD1 and expressed in Escherichia coli strain DH1
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
-
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
CoCl2
nearly complete inhibition
Escherichia coli
CuCl2
nearly complete inhibition
Escherichia coli
diethyl dicarbonate
complete
Escherichia coli
FeCl2
-
Escherichia coli
FeCl3
nearly complete inhibition
Escherichia coli
HgCl2
-
Escherichia coli
iodoacetic acid
70% remaining activity at concentration 1 mM
Escherichia coli
N-bromosuccinimide
complete
Escherichia coli
NiSO4
-
Escherichia coli
o-phenanthroline
70% remaining acitvity at concentration 1 mM
Escherichia coli
p-chloromercuribenzoate
80% remaining activity at concentration 0.3 mM
Escherichia coli
ZnCl2
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
taurochenodeoxycholic acid
-
Escherichia coli
0.43
-
chenodeoxycholic acid
-
Escherichia coli
1.2
-
cholic acid
-
Escherichia coli
1.25
-
glycocholic acid
-
Escherichia coli
2
-
taurocholic acid
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
4 * 28000, SDS-PAGE
Escherichia coli
120000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
strain HB101
-
Purification (Commentary)
Commentary
Organism
recombinant from Escherichia coli, high yield
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chenodeoxycholic acid + NAD(P)+
-
286085
Escherichia coli
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
?
cholic acid + NAD(P)+
-
286085
Escherichia coli
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
-
-
-
glycocholic acid + NAD+
-
286085
Escherichia coli
N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
-
-
?
additional information
no activity with deoxycholic acid and taurodeoxycholic acid
286085
Escherichia coli
?
-
-
-
-
taurochenodeoxycholic acid + NAD+
-
286085
Escherichia coli
2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
-
-
?
taurocholic acid + NAD+
-
286085
Escherichia coli
2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 28000, SDS-PAGE
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.417
-
glycocholic acid
-
Escherichia coli
3.13
-
taurocholic acid
-
Escherichia coli
3.43
-
taurochenodeoxycholic acid
-
Escherichia coli
8.18
-
chenodeoxycholic acid
-
Escherichia coli
63.3
-
cholic acid
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
9
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene from strain HB101 cloned in plasmid pSD1 and expressed in Escherichia coli strain DH1
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CoCl2
nearly complete inhibition
Escherichia coli
CuCl2
nearly complete inhibition
Escherichia coli
diethyl dicarbonate
complete
Escherichia coli
FeCl2
-
Escherichia coli
FeCl3
nearly complete inhibition
Escherichia coli
HgCl2
-
Escherichia coli
iodoacetic acid
70% remaining activity at concentration 1 mM
Escherichia coli
N-bromosuccinimide
complete
Escherichia coli
NiSO4
-
Escherichia coli
o-phenanthroline
70% remaining acitvity at concentration 1 mM
Escherichia coli
p-chloromercuribenzoate
80% remaining activity at concentration 0.3 mM
Escherichia coli
ZnCl2
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
taurochenodeoxycholic acid
-
Escherichia coli
0.43
-
chenodeoxycholic acid
-
Escherichia coli
1.2
-
cholic acid
-
Escherichia coli
1.25
-
glycocholic acid
-
Escherichia coli
2
-
taurocholic acid
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
4 * 28000, SDS-PAGE
Escherichia coli
120000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant from Escherichia coli, high yield
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chenodeoxycholic acid + NAD(P)+
-
286085
Escherichia coli
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
?
cholic acid + NAD(P)+
-
286085
Escherichia coli
3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
-
-
-
glycocholic acid + NAD+
-
286085
Escherichia coli
N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
-
-
?
additional information
no activity with deoxycholic acid and taurodeoxycholic acid
286085
Escherichia coli
?
-
-
-
-
taurochenodeoxycholic acid + NAD+
-
286085
Escherichia coli
2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
-
-
?
taurocholic acid + NAD+
-
286085
Escherichia coli
2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 28000, SDS-PAGE
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.417
-
glycocholic acid
-
Escherichia coli
3.13
-
taurocholic acid
-
Escherichia coli
3.43
-
taurochenodeoxycholic acid
-
Escherichia coli
8.18
-
chenodeoxycholic acid
-
Escherichia coli
63.3
-
cholic acid
-
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
9
-
Escherichia coli
Other publictions for EC 1.1.1.159
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697994
Fukiya
Conversion of cholic acid and ...
Bacteroides fragilis, Bacteroides fragilis NCTC 9343, Bacteroides intestinalis, Bacteroides intestinalis am-1, Escherichia coli HB101
FEMS Microbiol. Lett.
293
263-270
2009
6
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701240
Giovannini
7alpha- and 12alpha-Hydroxyste ...
Acinetobacter lwoffii
Steroids
73
1385-1390
2008
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670898
Pedrini
Xanthomonas maltophilia CBS 89 ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia CBS 897.97
Steroids
71
189-198
2006
-
1
-
-
-
-
-
2
-
-
-
1
-
3
-
-
1
-
-
-
2
1
7
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
2
1
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670926
Zhu
-
Enzymatic enantioselective red ...
Bacteroides fragilis
Tetrahedron
62
4535-4539
2006
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
-
16
-
1
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
16
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
656301
Ueda
-
Molecular and enzymatic proper ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Macromol.
4
33-38
2004
-
-
-
-
-
-
-
1
-
-
2
-
-
6
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655302
Bennett
Cloning and characterization o ...
Bacteroides fragilis, Clostridium scindens, Escherichia coli, Paeniclostridium sordellii
Curr. Microbiol.
47
475-484
2003
-
-
1
-
-
-
2
4
-
-
2
-
-
6
-
-
1
-
-
-
1
-
8
1
-
-
4
-
2
1
-
5
-
-
-
-
-
1
5
-
-
-
-
2
-
4
-
-
2
-
-
-
-
1
-
-
1
-
8
1
-
-
4
-
2
1
-
-
-
-
-
-
-
-
286083
Tanabe
Roles of the Ser146, Tyr159, a ...
Escherichia coli
J. Biochem.
124
634-641
1998
-
-
1
1
6
-
-
12
-
-
2
-
-
2
-
-
1
1
-
-
-
-
-
1
-
-
-
6
1
1
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
12
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
6
1
1
-
-
-
-
-
-
-
-
286081
Tanaka
Crystal structures of the bina ...
Escherichia coli
Biochemistry
35
7715-7730
1996
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286084
Coleman
Characterization and regulatio ...
Paeniclostridium sordellii
J. Bacteriol.
176
4865-4874
1994
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
1
-
-
1
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286082
Baron
Cloning, sequencing, and expre ...
Eubacterium sp., Eubacterium sp. VPI 12708
J. Bacteriol.
173
4558-4569
1991
-
-
1
-
-
-
-
-
-
-
1
4
-
12
-
-
1
-
-
-
2
-
8
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
2
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286085
Yoshimoto
Cloning and sequencing of the ...
Escherichia coli
J. Bacteriol.
173
2173-2179
1991
-
-
1
1
-
-
12
5
-
-
2
-
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
5
1
-
1
1
-
-
-
-
-
1
1
1
-
-
-
12
-
5
-
-
2
-
-
-
-
1
-
-
-
-
6
1
-
-
-
5
1
-
1
-
-
-
-
-
-
-
286072
Prabha
Purification of 7alpha-hydroxy ...
Escherichia coli
Can. J. Microbiol.
36
131-135
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
286073
Prabha
Kinetic properties of 7alpha-h ...
Escherichia coli
Can. J. Microbiol.
35
1076-1080
1989
2
-
-
-
-
-
18
-
-
4
-
-
-
1
-
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
18
-
-
-
4
-
-
-
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286074
Ottolina
Enzymatic synthesis of [4R-2H] ...
Escherichia coli
Biochim. Biophys. Acta
998
173-178
1989
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286075
Kinoshita
-
Purification and properties of ...
Brevibacterium fuscum
J. Ferment. Technol.
66
145-152
1988
-
-
-
-
-
-
-
8
-
-
2
-
-
1
-
-
1
-
-
-
4
-
8
1
1
1
2
-
3
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
8
-
-
2
-
-
-
-
1
-
-
4
-
8
1
1
1
2
-
3
-
2
-
-
-
-
-
-
-
286076
MacDonald
Bile induction of 7alpha- and ...
Clostridium sardiniense
Biochim. Biophys. Acta
665
262-269
1981
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286077
Haslewood
The specificity of a 7alpha-hy ...
Escherichia coli
Biochem. J.
157
207-210
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
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286078
Hylemon
Multiple forms of 7-alpha-hydr ...
Bacteroides fragilis
J. Bacteriol.
122
418-424
1975
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2
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2
2
1
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1
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1
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7
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2
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1
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2
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2
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2
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2
2
1
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1
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7
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2
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1
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286079
MacDonald
NAD- and NADP-dependent 7alpha ...
Bacteroides fragilis
Biochim. Biophys. Acta
384
12-24
1975
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1
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7
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3
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5
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2
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2
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1
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7
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5
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2
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286080
MacDonald
7alpha-hydroxysteroid dehydrog ...
Escherichia coli
Biochim. Biophys. Acta
309
243-253
1973
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2
3
6
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7
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6
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7
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